In vitro propagation of Alkanna tinctoria Tausch.: a medicinal plant of the Boraginaceae family with high pharmaceutical value

Cartabia, Annalisa; Sarropoulou, Virginia; Grigoriadou, Katerina; Maloupa, Eleni; Declerck, Stéphane

doi:10.1016/j.indcrop.2022.114860

Cited by 9 publications

(7 citation statements)

References 32 publications

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“…Furthermore, the combinations of BAP and NAA produced fewer shoots and leaves than the treatment with BAP alone. Notably, the combination of BAP at 1.0 mg/L and NAA at 1.0 mg/L produced the lowest numbers of shoots (4.9 shoots/explant) and leaves (3.1 leaves/explant) when cultured in liquid medium, and the established microshoots displayed abnormal and hyperhydricity symptoms, similar to the observation in Alkanna tinctoria cultured in medium supplemented with high concentrations of cytokinins and auxins [27]. Another study conducted by Ziv and Ariel [28] discovered that Philodendron hastatum burgundy leaves developed necrosis during the proliferation stage in agitated liquid culture.…”

Section: Effect Of Pgrs On Shoot Proliferationsupporting

confidence: 73%

In Vitro Propagation of Philodendron erubescens ‘Pink Princess’ and Ex Vitro Acclimatization of the Plantlets

et al. 2023

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This study describes the in vitro propagation and ex vitro acclimatization of Philodendron erubescens pink princess, one of the most popular ornamental variegated foliage plants. For shoot proliferation, the protocorm-like bodies of the Philodendron pink princess were cultured on solid Murashige and Skoog (MS) media supplemented with 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA) at different concentrations. The results revealed that supplementation with BAP alone at a concentration of 1.0 mg/L yielded the maximum number of shoots and leaves. Furthermore, the application of BAP at 1.0 mg/L significantly enhanced the shoot proliferation of Philodendron pink princess when grown in liquid MS medium, yielding 11.2 shoots/explant and 4.7 leaves/explant. When the established microshoots were subjected to root induction using solid MS media supplemented with different kinds and concentrations of auxins, indole-3-butyric acid (IBA) at 3 mg/L resulted in the highest number of roots (3.2 roots/explant) and longest root length (1.9 cm). Three supporting materials, i.e., peat moss, vermiculite, and perlite, were used as planting media for the ex vitro acclimatization of the Philodendron pink princess plantlets. The results demonstrated that the in vitro plantlets acclimatized and exhibited a relatively high survival frequency in all planting media without morphological abnormalities. Peat moss outperformed all other types of planting media in terms of sustaining the vegetative growth of the plantlets. In the future, the approach established in this study could be employed for the extensive production of Philodendron pink princess.

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Section: Effect Of Pgrs On Shoot Proliferationsupporting

confidence: 73%

In Vitro Propagation of Philodendron erubescens ‘Pink Princess’ and Ex Vitro Acclimatization of the Plantlets

et al. 2023

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“…In those protocols, Murashige and Skoog medium has been supplemented with combinations of cytokinins and auxins such as 2,4D (2,4-dichlorophenoxyacetic acid), IBA (Indole Butyric Acid), BA (6-benzyl adenine), BAP (6-benzyl aminopurine), NAA (Naphthalene acetic acid), IAA (Indole-3-acetic acid), TDZ (Thidiazuron), Kinetin and Topolin. Among these, 2,4D-BA or 2,4D-IBA are the most commonly utilized [ 32 , 33 , 34 , 35 , 48 , 49 , 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Martínez-Rodríguez

Beltran-Garcia

Salas

et al. 2022

Biology

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A. marmorata is the raw material used for tepextate mescal production but is classified as an endangered species. In the present study, we obtain and multiply clonal lines of Agave marmorata Roezl by selecting seedlings derived from seeds. Ten seedlings from two lots of 400 germinated seeds were selected for axillary bud proliferation induced by BAP 5 mg/L in vitamin-free Murashige and Skoog’s medium. Differences in shoot numbers, heights and senescent tissue formation were observed. Notably, the AM32 line formed 84 shoots and presented low senescent tissue after 60 d of culture. We also selected the AM31 and AM33 clonal lines. Four-month shoots were extracted with 80% methanol in water to determine the total content of saponins, flavonoids, and phenolic acids and compare the three clonal lines. Some bioactive molecules were identified using HPLC techniques and MALDI-TOF mass spectrometry none showed significant differences in content. Additionally, plants derived from the clonal lines were inoculated with four endophytic bacteria. Among these, Achromobacter xylosoxidans supported plant growth of AM32. A notable effect of plant death was observed after inoculation with Enterobacter cloacae, an endophyte of A. tequilana. Additionally, Pseudomonas aeruginosa, an endophyte from A. marmorata, reduced biomass. Our results demonstrate the incompatibility of A. marmorata to E. cloacae and specialization between the host plant and its endophytes. The compatibility of the plant-endophyte could be exploited to boost the establishment and stability of mutualisms to benefit plant development, stress tolerance and pathogen resistance. The differences in multiplication capacity, stable tissue formation, and endophyte biotization responses may indicate genetic variability. Clonal selection and micropropagation from seed-derived plants could contribute to conserving the endangered A. marmorata plant for reforestation in their natural habitats, thus, assuring mass propagation for sustainable industrial production of mescal, bioactive compounds, and prebiotics.

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“…Alkanna tinctoria unrooted in vitro explants (International Plant Exchange Network – IPEN – accession number GR-1-BBGK-17 ,5975) were provide by IPBGR, HAO Demeter (Thessaloniki, Greece). The plants were proliferated on Murashige-Skoog (MS) basal medium enriched with plant growth regulators (PGRs) and rooted on Root Culture 1 (RC1) modified medium free of ammonium nitrate ( Cartabia et al, 2022 ). The rooted A. tinctoria plants were further acclimatized following different steps resulting in an optimal plant survival rate (see section “ Alkanna tinctoria acclimatization protocol” in Supplementary material and Supplementary Figure 5 ).…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, this plant is characterized by a very low seed germination rate and its cultivation under conventional agriculture practices is hampered by several constraints (e.g., time needed for A/S production, high costs of harvesting, and exposure to biotic/abiotic stresses) ( Malik et al, 2016 ). For these reasons, in vitro and ex vitro cultivation protocols have been developed for the propagation and preservation of this plant ( Cartabia et al, 2022 ). In addition, hydroponic cultivation of medicinal plants has attracted the attention of the scientific community and industrial sector, as it can effectively meet the nutrients needs of the plants under controlled/stable environmental conditions ( Gontier et al, 2002 ; Sgherri et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Arbuscular mycorrhizal fungi impact the production of alkannin/shikonin and their derivatives in Alkanna tinctoria Tausch. grown in semi-hydroponic and pot cultivation systems

et al. 2023

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IntroductionAlkanna tinctoria Tausch. is a medicinal plant well-known to produce important therapeutic compounds, such as alkannin/shikonin and their derivatives (A/Sd). It associates with arbuscular mycorrhizal fungi (AMF), which are known, amongst others beneficial effects, to modulate the plant secondary metabolites (SMs) biosynthesis. However, to the best of our knowledge, no study on the effects of AMF strains on the growth and production of A/Sd in A. tinctoria has been reported in the literature.MethodsHere, three experiments were conducted. In Experiment 1, plants were associated with the GINCO strain Rhizophagus irregularis MUCL 41833 and, in Experiment 2, with two strains of GINCO (R. irregularis MUCL 41833 and Rhizophagus aggregatus MUCL 49408) and two native strains isolated from wild growing A. tinctoria (R. irregularis and Septoglomus viscosum) and were grown in a semi-hydroponic (S-H) cultivation system. Plants were harvested after 9 and 37 days in Experiment 1 and 9 days in Experiment 2. In Experiment 3, plants were associated with the two native AMF strains and with R. irregularis MUCL 41833 and were grown for 85 days in pots under greenhouse conditions. Quantification and identification of A/Sd were performed by HPLC-PDA and by HPLC-HRMS/MS, respectively. LePGT1, LePGT2, and GHQH genes involved in the A/Sd biosynthesis were analyzed through RT-qPCR.ResultsIn Experiment 1, no significant differences were noticed in the production of A/Sd. Conversely, in Experiments 2 and 3, plants associated with the native AMF R. irregularis had the highest content of total A/Sd expressed as shikonin equivalent. In Experiment 1, a significantly higher relative expression of both LePGT1 and LePGT2 was observed in plants inoculated with R. irregularis MUCL 41833 compared with control plants after 37 days in the S-H cultivation system. Similarly, a significantly higher relative expression of LePGT2 in plants inoculated with R. irregularis MUCL 41833 was noticed after 9 versus 37 days in the S-H cultivation system. In Experiment 2, a significant lower relative expression of LePGT2 was observed in native AMF R. irregularis inoculated plants compared to the control.DiscussionOverall, our study showed that the native R. irregularis strain increased A/Sd production in A. tinctoria regardless of the growing system used, further suggesting that the inoculation of native/best performing AMF is a promising method to improve the production of important SMs.

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In vitro propagation of Alkanna tinctoria Tausch.: a medicinal plant of the Boraginaceae family with high pharmaceutical value

Cited by 9 publications

References 32 publications

In Vitro Propagation of Philodendron erubescens ‘Pink Princess’ and Ex Vitro Acclimatization of the Plantlets

In Vitro Propagation of Philodendron erubescens ‘Pink Princess’ and Ex Vitro Acclimatization of the Plantlets

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Arbuscular mycorrhizal fungi impact the production of alkannin/shikonin and their derivatives in Alkanna tinctoria Tausch. grown in semi-hydroponic and pot cultivation systems

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