In vitro propagation of Acacia species—a review

Vengadesan, G.; Ganapathi, A.; Amutha, S.; Selvaraj, N.

doi:10.1016/s0168-9452(02)00144-9

Cited by 49 publications

(38 citation statements)

References 36 publications

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“…Nemeth (1986) reported that multiple shoots regeneration depends on many endogenous and exogenous factors such as genetic background, physiological influences, age, and the ontogenetic phase of the mother plant, environment, and composition of the nutrient medium. Organogenesis is commonly induced by manipulation of exogenous phytohormone levels and occurs either directly from explant tissue or through callus (Vengadesan et al, 2002). Our results are in accordance with previous studies, suggesting that TDZ is among the most active cytokinin-like growth substances for tissue culture of woody plant species and its low concentrations induces axillary shoot proliferation but may inhibit shoot elongation and high concentrations promotes formation of adventitious shoots (Heutteman and Preece, 1993).…”

Section: Discussionsupporting

confidence: 92%

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Micropropagation of Myrica rubra Sieb. and Zucc. using shoot tips and nodal explant

Asghari¹,

Abbas²,

Chen³

et al. 2013

Afr. J. Agric. Res.

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Section: Discussionsupporting

confidence: 92%

“…Micropropagation is used for faster multiplication of true-to-type genotypes in the minimum possible time (Vengadesan et al, 2002). Plants through micropropagation show greater genetic uniformity and clonal fidelity (Martin, 2003).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Myrica rubra Sieb. and Zucc. using shoot tips and nodal explant

Asghari¹,

Abbas²,

Chen³

et al. 2013

Afr. J. Agric. Res.

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“…The GA 3 removal from the medium gave a good multiplication rate and quality of the propagated shoots. This has also been observed in plants belonging to the Fabaceae family [24][25] cultured on medium with BA as unique growth regulator.…”

Section: Discussionsupporting

confidence: 54%

Cytisus aeolicus Guss. ex Lindl. in vitro cultures and genistin production

et al. 2010

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Cytisus aeolicus Guss. ex Lindl. (Fabaceae family, subfamily Faboideae) is an endangered endemic species of the Aeolian Islands, Sicily. In vitro multiplication of C. aeolicus shoots was described in this work and cell cultures were established from cotyledons and hypocotyls to investigate their potential production of isoflavones. Aseptically germinated seeds, cultivated on LS modified basal medium, gave the initial explants used both to induce axillary propagation and callus cultures. The LS (Linsmaier and Skoog) basal medium, supplemented with 0.1 mg L -1 of 6-benzylaminopurine were used to induce axillary propagation. The callus induction was performed using the basal medium added with 5 mg L -1 2,4-dichlorophenoxy acetic acid and 5 mg L -1 kinetin (control medium). Basal medium was also added with 2000 mg L -1 casein hydrolysate (CH) or 900 mg L -1 myo-inositol (MI). C. aeolicus callus cultures on CH and MI media produced an unique compound, the isoflavone genistein 7-O-ß-D-glucopyranoside (genistin), which has not previously been isolated from wild plants. Callus cultures grown on the medium containing myo-inositol produced the greatest amount of genistin. C. aeolicus tissue culture procedures could provide suitable plant material both for germplasm preservation (by micropropagation) and for biotechnological selective isoflavone production (by callus culture).

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“…It prevents the production of polyphenols which often hinder organogenesis and cause explant necrosis. For in vitro propagation of Acacia plantlets, citric acid was the most effective in supporting axillary shoot proliferation and preventing browning of the medium [25].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l -1 citric acid, 12.2 mg.l -1 adenine sulfate, 15 mg.l -1 L-arginine, 2.46 M IBA (indole-3-butyric acid), 30 g.l -1 sucrose and 7 g.l -1 of agar, and enriched with different balances of BA (benzyladenine) and L-glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 M BA and 25 mg.l -1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in an MS medium supplemented with 2.21 M BA, 5.70 M IAA (indole-3-acetic acid) and 15 mg.l -1 L-arginine. These shoots were isolated and then rooted in MS containing 2.46 M of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable.

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In vitro propagation of Acacia species—a review

Cited by 49 publications

References 36 publications

Micropropagation of Myrica rubra Sieb. and Zucc. using shoot tips and nodal explant

Micropropagation of Myrica rubra Sieb. and Zucc. using shoot tips and nodal explant

Cytisus aeolicus Guss. ex Lindl. in vitro cultures and genistin production

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

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