In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants

Husain, Mohd Kashif; Anis, Mohammad; Shahzad, Anwar

doi:10.1007/s11738-007-0130-6

Cited by 58 publications

(46 citation statements)

References 26 publications

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“…The shoot proliferation rate was significantly affected by addition of Gln, ADS and CH. The simulative role of ADS in shoot multiplication has been emphasized from time to time in various plants (Husain and Anis, 2004;Dhar and Upreti, 1999;Husain et al, 2008). In our study, we found 50 mg/L of ADS as highly effective for enhancing the shoot multiplication rate (4.88 shoots per explant).…”

Section: Discussionsupporting

confidence: 61%

Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)

Siwach¹,

Swati²,

Anita³

et al. 2012

Afr. J. Biotechnol.

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Section: Discussionsupporting

confidence: 61%

Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)

Siwach¹,

Swati²,

Anita³

et al. 2012

Afr. J. Biotechnol.

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“…In their study, reduced BA concentration (0.5 mg L -1 ) was found to be effective for shoot proliferation. The simulative role of Ads in shoot multiplication has been emphasized from time to time in various plants [26,27]. Adenine sulphate, supplied in combination with BA and IAA, enhanced the shoot multiplication by many folds during the present investigation.…”

Section: Resultsmentioning

confidence: 50%

In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

et al. 2014

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An efficient protocol of plant regeneration through direct and indirect organogenesis in Swertia chirayita was developed. Explants cultured on Murashige and Skoog medium supplemented with 2,4-D (0.5 mg L−1) with combination of Kinetin (0.5 mg L−1) showed the highest frequency (84%) of callusing and 1.0mg L−1 6-benzyladenine (BA) in combination with (100 mg L−1) Adenine sulphate (Ads) + (0.1 mg L−1) Indole acetic acid (IAA) was excellent for maximum adventitious shoot (12.69 ± 1.30) formation in four week of culture. A maximum number of (7.14 ± 0.99) shoots were developed per leaf explants through direct organogenesis. The highest frequency of rooting (11.46 ± 1.56) was observed on MS medium augmented with IAA (1.0 mg L−1). Well-rooted shoots transferred to plastic pots containing a soilrite: sand mix and then moved to the greenhouse for further growth and development. Four major secondary metabolites were analyzed and quantified using high performance liquid chromatography. Amount of secondary metabolites was found significantly higher, in in vitro plantlets compared to in vivo plantlets and callus raised from S. chirayita. Higher heavy metal accumulation in in vitro as compared to in vivo plantlets correlates higher secondary metabolite production supporting that they play regulatory role in influencing the plant secondary metabolism.

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“…3). A similar two-step rooting procedure has been used successfully for different species (Martin, 2003;Feyissa et al, 2005;Siddique and Anis, 2007;Husain et al, 2008;Conde et al, 2008). IBA at high concentration is known to suppress root elongation (Korach et al, 2002).…”

Section: Resultsmentioning

confidence: 99%

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

Trejgell¹,

Tretyn²

2011

Acta Biologica Cracoviensia Series Botanica

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This paper is the first published report describing micropropagation of Carlina onopordifolia, using shoot tip and hypocotyl explants. The explants were excised from 10-day-old seedlings and transferred to proliferation medium supplemented with 6-benzylaminopurine (BA; 1.0 or 3.0 mg l -1 ), kinetin (Kn; 1.0 or 3.0 mg l -1 ) or zeatin (ZEA; 1.0 or 3.0 mg l -1 ) in combination with naphthaleneacetic acid (NAA; 0.1 mg l -1 ). The shoot tips were significantly better than hypocotyls as initial material for shoot regeneration. For shoot multiplication, MS medium supplemented with BA proved superior to the other cytokinins tested. Medium supplemented with 1.0 mg l -1 BA gave the highest shoot propagation frequency (66.9%) and number of shoots per explant (2.5). Single shoots were separated from each other and rooted on MS supplemented with IBA for the whole period of culture, with longor short-pulse IBA application. The highest rooting frequency (84.8%) and root number (18.8) were for shortpulse (1 min) 1000 mg l -1 IBA solution. The higher IBA concentration stimulated callus formation and the development of short roots. The shoots were transferred to MS medium without growth regulators. Survival was highest (54.4%) for the plants from the short-pulse 100 mg l -1 IBA treatment. After 8 weeks of acclimatization the plantlets were removed to field conditions and grew normally.K Ke ey y w wo or rd ds s: : Carlina onopordifolia, micropropagation, cytokinins, rooting, auxin.

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In vitro propagation of a multipurpose leguminous tree (Pterocarpus marsupium Roxb.) using nodal explants

Cited by 58 publications

References 26 publications

Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)

Effects of adenine sulphate, glutamine and casein hydrolysate on in vitro shoot multiplication and rooting of Kinnow mandarin (Citrus reticulata Blanco)

In vitro organogenesis secondary metabolite production and heavy metal analysis in Swertia chirayita

Shoot Multiplication and in Vitro Rooting of Carlina onopordifolia Basser

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