In vitro propagation, genetic stability, and secondary metabolite analysis of wild lavender (Lavandula coronopifolia Poir.)

Khateeb, Wesam Al; Kanaan, Razan; El‐Elimat, Tamam; Alu’datt, Muhammad H.; Lahham, Jamil N.; El-Oqlah, Ahmad

doi:10.1007/s13580-017-0342-7

Cited by 12 publications

(9 citation statements)

References 24 publications

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“…DPPH method: The DPPH method was used for in-vitro antioxidant activity of pure AN and optimized AN-LC-CS-TPGS-NPs (ON2) as per the prescribed procedure with a slight modification [ 29 ]. The pure AN and the ON2 samples were prepared in methanol at a concentration of 1 mg/mL and the final concentration was diluted between 10–100 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

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Development and Optimization of Hybrid Polymeric Nanoparticles of Apigenin: Physicochemical Characterization, Antioxidant Activity and Cytotoxicity Evaluation

Zafar

Alruwaili

Imam

et al. 2022

Sensors

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Breast cancer is the most common cancer in females and ranked second after skin cancer. The use of natural compounds is a good alternative for the treatment of breast cancer with less toxicity than synthetic drugs. The aim of the present study is to develop and characterize hybrid Apigenin (AN) Nanoparticles (NPs) for oral delivery (AN-NPs). The hybrid AN-NPs were prepared by the self-assembly method using lecithin, chitosan and TPGS. Further, the NPs were optimized by Box-Behnken design (3-factor, 3-level). The hybrid NPs were evaluated for particle size (PS), entrapment efficiency (EE), zeta potential (ZP), and drug release. The optimized hybrid NPs (ON2), were further evaluated for solid state characterization, permeation, antioxidant, cytotoxicity and antimicrobial study. The formulation (ON2) exhibited small PS of 192.6 ± 4.2 nm, high EE 69.35 ± 1.1%, zeta potential of +36.54 mV, and sustained drug release (61.5 ± 2.5% in 24 h), as well as significantly (p < 0.05) enhanced drug permeation and antioxidant activity. The IC50 of pure AN was found to be significantly (p < 0.05) lower than the formulation (ON2). It also showed significantly greater (p < 0.05) antibacterial activity than pure AN against Bacillus subtilis and Salmonella typhimurium. From these findings, it revealed that a hybrid AN polymeric nanoparticle is a good carrier for the treatment of breast cancer.

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Section: Methodsmentioning

confidence: 99%

“…The antioxidant activity was measured by a UV-spectrophotometer, the UV-Visible spectrophotometer at 571 nm. A comparative analysis of a control (ct) sample without DPPH analysis was also done and % scavenging activity was determined by the formula [ 29 ].

…”

Section: Methodsmentioning

confidence: 99%

Development and Optimization of Hybrid Polymeric Nanoparticles of Apigenin: Physicochemical Characterization, Antioxidant Activity and Cytotoxicity Evaluation

Zafar

Alruwaili

Imam

et al. 2022

Sensors

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“…The in-vitro antioxidant activity of pure GS and ternary inclusion complex (GS-TC) was analyzed by the reported DPPH method with slight modification [29]. The standard stock solution of pure GS and GS TC (1 mg/mL) was prepared in methanol.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Formulation of Genistein-HP β Cyclodextrin-Poloxamer 188 Ternary Inclusion Complex: Solubility to Cytotoxicity Assessment

et al. 2021

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The current study was designed to prepare the inclusion complex Genistein (GS) using Hydroxypropyl β cyclodextrin (HP β CD) and poloxamer 188 (PL 188). The binary inclusion complex (GS BC) and ternary inclusion complex (GS TC) were developed by microwave irradiation technique and evaluated for a comparative dissolution study. Further, the samples were assessed for FTIR, DSC, XRD, and NMR for the confirmation of complex formation. Finally, antioxidant and antimicrobial studies and cytotoxicity studies on a breast cancer (MCF-7) cell line were conducted. The dissolution study result showed a marked increment in GS dissolution/release after incorporation in binary (GS: HP β CD, 1:1) and ternary (GS: HP β CD: PL 188; 1:1:0.5) inclusion complexes. Moreover, the ternary complex exhibited a significant enhancement (p < 0.05) in dissolution than did the binary complexes. This might be due to the presence of PL 188, which helps in solubility enhancement of GS. DSC, XRD and SEM evaluation confirmed the modification in the structure of GS. FTIR and NMR results indicated the formation of an inclusion complex. The antioxidant and antimicrobial activity results revealed that GS TC has shown significant (p < 0.05) higher activity than pure GS. The cytotoxicity study results also depicted concentration-dependent cytotoxicity. GS TC exhibited significantly (p < 0.05) high cytotoxicity to cancer cells (IC50 = 225 µg/mL) than pure GS (IC50 = 480 µg/mL). Finally, it was concluded that a remarkable enhancement in the dissolution was observed after the inclusion of GS in the ternary complex and it therefore has significant potential for the treatment of breast cancer.

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“…Aasim et al [12] also recorded the maximum number and length of shoots per explant of cowpea on MS containing 0.5 mg l 21 BAP with 0.1 mg l 21 NAA. In contrast, Bekele et al [26] reported the highest number of shoot regeneration in Coccinia abyssinica on MS containing 2.0 mg l 21 BAP alone and Al Khateeb et al [27] reported in Lavandula coronopifolia on MS containing 0.5 mg l 21 BAP. Different concentrations of NAA and IBA were used in ½ MS medium for rooting from excised shoots.…”

Section: Discussionmentioning

confidence: 93%

“…Shekhawat et al [15] reported the best root induction and elongation of Coccinia indica on ½ MS12.0 mg l 2 1IBA. Al Khateeb et al [27] observed the maximum number of root induction in Lavandula coronopifolia at half-strength MS with 1.0 mg l 21 IBA. In contrast, Hassan et al [30] reported the highest number of root induction in Phlogacanthus thyrsiflorus on half-strength MS with 0.5 mg l 21 IBA and 0.5 mg l 21 IAA together.…”

Section: Discussionmentioning

confidence: 99%

Virus-Free Plant Regeneration from Shoot Apical Meristem of Coccinia grandis L., an Important Medicinal Plant in Bangladesh

Hasan¹,

Jannat²,

Zaman³

et al. 2019

Recent Advances in Biology and Medicine

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This investigation was undertaken to establish an efficient protocol for virus-free plant regeneration in Coccinia grandis L. through shoot apical meristem culture. Murashige and Skoog (MS) basal medium supplemented with different concentrations and combinations of 6-benzyl amino purine (BAP), gibberellic acid (GA3), naphthalene acetic acid (NAA), and indole-3-butyric acid (IBA) was used for meristem establishment, shoot regeneration, and root induction as well as elongation. MS liquid medium supplemented with 1.0 mg l-1 BAP + 0.10 mg l-1 NAA was found to be the best medium for the primary establishment of meristems. MS medium containing 1.5 mg l-1 BAP + 0.5 mg l-1 GA3 + 0.5 mg l-1 NAA was found to be best for shoot regeneration percentage at 100.0 ± 0.0 and multiplication with 10.0 ± 0.8 shoots per meristem as well as shoot elongation (highest 9.0 ± 0.0 cm). In vitro grown shoots were subcultured and rooted with 11.0 ± 0.8 roots per shoot subsequently on MS medium containing 0.5 mg l-1 IBA. Well-rooted plantlets were gradually acclimatized and successfully established in the field condition with 100% survival rate.

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In vitro propagation, genetic stability, and secondary metabolite analysis of wild lavender (Lavandula coronopifolia Poir.)

Cited by 12 publications

References 24 publications

Development and Optimization of Hybrid Polymeric Nanoparticles of Apigenin: Physicochemical Characterization, Antioxidant Activity and Cytotoxicity Evaluation

Development and Optimization of Hybrid Polymeric Nanoparticles of Apigenin: Physicochemical Characterization, Antioxidant Activity and Cytotoxicity Evaluation

Formulation of Genistein-HP β Cyclodextrin-Poloxamer 188 Ternary Inclusion Complex: Solubility to Cytotoxicity Assessment

Virus-Free Plant Regeneration from Shoot Apical Meristem of Coccinia grandis L., an Important Medicinal Plant in Bangladesh

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