In Vitro Propagation for Commercial Production of Ornamentals

Smith, Roberta H.

doi:10.1016/b978-0-12-415920-4.00012-8

Cited by 6 publications

(1 citation statement)

References 45 publications

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“…The propagation medium was MS + BA (1.0 mg/L) + NAA (0.03 mg/L), and the rooting medium was 1/2MS + IBA (0.5 mg/L). After the aseptic seedlings of S. sphenanthera had taken root (see Figure S11A,B), the plants with the same growth momentum were transplanted into the sterilized soil in a culture bottle (see Figure S11C) [40]. Tissue cultures of S. sphenanthera were planted in a small bottle with sterilized soil, on the basis of Liu et al [25].…”

Section: Syncom Construction and Functional Evaluationmentioning

confidence: 99%

Plant Triterpenoids Regulate Endophyte Community to Promote Medicinal Plant Schisandra sphenanthera Growth and Metabolites Accumulation

You

Qin

Wang

et al. 2021

JoF

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Beneficial interactions between endophytes and plants are critical for plant growth and metabolite accumulation. Nevertheless, the secondary metabolites controlling the feedback between the host plant and the endophytic microbial community remain elusive in medicinal plants. In this report, we demonstrate that plant-derived triterpenoids predominantly promote the growth of endophytic bacteria and fungi, which in turn promote host plant growth and secondary metabolite productions. From culturable bacterial and fungal microbial strains isolated from the medicinal plant Schisandra sphenanthera, through triterpenoid-mediated screens, we constructed six synthetic communities (SynComs). By using a binary interaction method in plates, we revealed that triterpenoid-promoted bacterial and fungal strains (TPB and TPF) played more positive roles in the microbial community. The functional screening of representative strains suggested that TPB and TPF provide more beneficial abilities to the host. Moreover, pot experiments in a sterilized system further demonstrated that TPB and TPF play important roles in host growth and metabolite accumulation. In summary, these experiments revealed a role of triterpenoids in endophytic microbiome assembly and indicated a strategy for constructing SynComs on the basis of the screening of secondary metabolites, in which bacteria and fungi join forces to promote plant health. These findings may open new avenues towards the breeding of high yielding and high metabolite-accumulating medicinal plants by exploiting their interaction with beneficial endophytes.

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Section: Syncom Construction and Functional Evaluationmentioning

confidence: 99%