In vitro propagation and phytochemical assessment of Perovskia abrotanoides Karel. (Lamiaceae) – A medicinally important source of phenolic compounds

Ghaderi, Shahla; Ebrahimi, Samad Nejad; Ahadi, Hamid; Moghadam, Sara E.; Mirjalili, Mohammad Hossein

doi:10.1016/j.bcab.2019.101113

Cited by 18 publications

(9 citation statements)

References 39 publications

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“…In the glasshouse-cultivated rosemary plants, the RA content was found to be constant over a period of one year under southern UK conditions [ 70 ], while glasshouse-cultivated plants in Spain showed the highest amounts of RA in February [ 69 ]. Although several reports of phenolic compounds and their derivatives in S. yangii and S. abrotanoides have been released to date [ 36 , 56 , 57 , 58 , 59 ], none of them investigated their content in different plant organs or variations throughout the vegetation season.…”

Section: Discussionmentioning

confidence: 99%

“…Besides tanshinones, another important chemical class of a pharmacological value in the subgenus Perovskia is phenolic compounds. There are only several reports of phenolic compounds and their derivatives in S. yangii and S. abrotanoides [ 36 , 56 , 57 , 58 , 59 ], but none of them investigated their content in different plant organs or variations throughout the vegetation season.…”

Section: Introductionmentioning

confidence: 99%

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Seasonal Variations of Rosmarinic Acid and Its Glucoside and Expression of Genes Related to Their Biosynthesis in Two Medicinal and Aromatic Species of Salvia subg. Perovskia

Stafiniak

Ślusarczyk

Pencakowski

et al. 2021

Biology

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Salvia abrotanoides Kar. and Salvia yangii B.T. Drew are medicinal and aromatic plants belonging to the subgenus Perovskia and used as herbal medicines in Asia. Derivatives of caffeic acid, mainly rosmarinic acid (RA), are the major phenolic compounds identified in these plants. Understanding the factors and molecular mechanisms regulating the accumulation of pharmacologically and ecologically relevant phenolic metabolites is essential for future biotechnological and medical applications. Up to date, no studies of phenylpropanoid biosynthetic pathway at the transcriptional level has been performed in the Perovskia subgenus. Using a combined qRT-PCR transcriptional activity analysis with LC-MS based metabolic profiling of roots and leaves at the beginning, in the middle and at the end of vegetation season, we have identified the following gene candidates with properties correlating to phenolic acid biosynthesis in S. abrotanoides and S. yangii: PAL, C4H, 4CL, TAT, HPPR, RAS1, RAS2 and Cyp98A14. A comparison of phenolic acid profiles with gene transcript levels revealed the transcriptional regulation of RA biosynthesis in the roots but not the leaves of the studied species. Additionally, RAS1 and Cyp98A14 were identified as rate-limiting steps regulating phenylpropanoid biosynthesis on a transcription level. In the future, this will facilitate the gene-based metabolic enhancement of phenolic compounds production in these promising medicinal herbs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seasonal Variations of Rosmarinic Acid and Its Glucoside and Expression of Genes Related to Their Biosynthesis in Two Medicinal and Aromatic Species of Salvia subg. Perovskia

Stafiniak

Ślusarczyk

Pencakowski

et al. 2021

Biology

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“…S. abrotanoides and S. yangii display a wide array of bioactive natural compounds belonging to different chemical classes [5]. Apart from essential oil constituents [18][19][20][21] and phenolic compounds [1,[22][23][24][25], they produce also a wide range of abietane-type diterpenoids. Aerial parts of S. abrotanoides and S. yangii contain carnosic acid, rosmanol and their derivatives [26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis of two Asian Medicinal and Aromatic Species of Salvia subg. Perovskia – a Contribution Towards Molecular Background of Abietane-type Diterpenoid Biosynthesis

Bielecka,

Stafiniak,

Pencakowski

et al. 2023

Preprint

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Tanshinones, are a group of diterpenoid red pigments present in Danshen – an important herbal drug of Traditional Chinese Medicine which is a dried root of Salvia miltiorrhiza Bunge. Some of the tanshinones are sought after as pharmacologically active natural products. To date, the biosynthetic pathway of tanshinones has been only partially elucidated. These compounds are also present in some of the other Salvia species, i.a. from subgenus Perovskia, such as S. abrotanoides (Kar.) Sytsma and S. yangii B.T. Drew. Despite of the close genetic relationship between these species, significant qualitative differences in their diterpenoid profile have been discovered. In this work, we have used the Liquid Chromatography–Mass Spectrometry analysis to follow the content of diterpenoids during the vegetation season, which confirmed our previous observations of a diverse diterpenoid profile. As metabolic differences are reflected in different transcript profile of a species or tissues, we used metabolomics-guided transcriptomic approach to select candidate genes, which expression possibly led to observed chemical differences. Using an RNA-sequencing technology we have sequenced and de novo assembled transcriptomes of leaves and roots of S. abrotanoides and S. yangii. As a result, 134,443 transcripts were annotated by UniProt and 56,693 of them were assigned as Viridiplantae. In order to seek for differences, the differential expression analysis was performed, which revealed that 463, 362, 922 and 835 genes indicated changes in expression in four comparisons. GO enrichment analysis and KEGG functional analysis of selected DEGs were performed. The homology and expression of two gene families, associated with downstream steps of tanshinone and carnosic acid biosynthesis were studied, namely: cytochromes P-450 and 2-oxoglutarate-dependend dioxygenases. Additionally, BLAST analysis revealed existence of 39 different transcripts related to abietane diterpenoid biosynthesis in transcriptomes of S. abrotanoides and S. yangii. We have used quantitative real-time RT-PCR analysis of selected candidate genes, to follow their expression levels over the vegetative season. A hypothesis of an existence of a multifunctional CYP76AH89 in transcriptomes of S. abrotanoides and S. yangii is discussed and potential roles of other CYP450 homologs are speculated. By using the comparative transcriptomic approach, we have generated a dataset of candidate genes which provides a valuable resource for further elucidation of tanshinone biosynthesis. In a long run, our investigation may lead to optimization of diterpenoid profile in S. abrotanoides and S. yangii, which may become an alternative source of tanshinones for further research on their bioactivity and pharmacological therapy.

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“…Additionally, destructive harvesting may result in resource exhaustion and even nearly extinction from the nature [27]. More importantly, the degree functionality of active ingredient also varies upon environmental situations [28]. Due to these constraints, the sustainable production of raw material should be considered [27].…”

Section: Introductionmentioning

confidence: 99%

“…Plant tissue culture (PTC) is a potential alternative method to traditional plant propagation for retrieving natural products in the ways that multiplying and maintaining higher consistency and greater true-to-type raw material [29]. Moreover, this in vitro plant regeneration is also simple and time saving [28]. The underlaying advantage is that it can also help reducing the invasion of the natural resources and the destruction of biodiversity [30].…”

Section: Introductionmentioning

confidence: 99%

Natural Surfactant Saponin from Tissue of Litsea glutinosa and Its Alternative Sustainable Production

Wisetkomolmat

Suksathan²,

Puangpradab³

et al. 2020

Plants

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In this research, we assessed the detergency properties along with chemical characteristic of the surfactant extracts from the most frequently cited detergent plants in Northern Thailand, namely, Sapindus rarak, Acacia concinna, and Litsea glutinosa. Moreover, as to provide the sustainable option for production of such valuable ingredients, plant tissue culture (PTC) as alternative method for industrial metabolite cultivation was also proposed herein. The results illustrated that detergent plant extracts showed moderate in foaming and detergency abilities compared with those of synthetic surfactant. The phytochemical analysis illustrated the positive detection of saponins in L. glutinosa plant extracts. The highest callus formation was found in L. glutinosa explant cultured with MS medium supplemented with 2.0 mg/L Indole-3-acetic acid (IAA). The callus extract was chemical elucidated using chromatography, which illustrated the presence of saponin similar to those from the crude leaf and Quillaja saponin extracts. Compact mass spectrometry confirmed that the surfactant was of the steroidal diagnostic type.

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In vitro propagation and phytochemical assessment of Perovskia abrotanoides Karel. (Lamiaceae) – A medicinally important source of phenolic compounds

Cited by 18 publications

References 39 publications

Seasonal Variations of Rosmarinic Acid and Its Glucoside and Expression of Genes Related to Their Biosynthesis in Two Medicinal and Aromatic Species of Salvia subg. Perovskia

Seasonal Variations of Rosmarinic Acid and Its Glucoside and Expression of Genes Related to Their Biosynthesis in Two Medicinal and Aromatic Species of Salvia subg. Perovskia

Comparative Transcriptomic Analysis of two Asian Medicinal and Aromatic Species of Salvia subg. Perovskia – a Contribution Towards Molecular Background of Abietane-type Diterpenoid Biosynthesis

Natural Surfactant Saponin from Tissue of Litsea glutinosa and Its Alternative Sustainable Production

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