In vitro propagation and cryopreservation of Romanian endemic and rare Hypericum species

Abstract

The preservation and reproduction of gene resources of rare and endemic rowan species were the main aims of this study. Rowan species represent important woody trees from the aspect of forest biodiversity. There are only several endemic rowan species in the Czech Republic which are of hybridogenous origin from the whitebeam (Sorbus aria) range. Most of them have been described only at the end of the 20 th century. For these species, the new procedures of vegetative reproduction were developed. In vitro cultures from dormant buds of 57 mature trees were established. The successful induction of organogenesis was achieved in a MS modified medium with 0.2 mg·l-1 of BAP, 0.1 mg·l-1 of IBA, 200 mg·l-1 of glutamine, 2 mg·l-1 of glycine, 200 mg·l-1 of casein hydrolysate, 30 mg·l-1 of sucrose, and 6 mg·l-1 of agar. The pH was adjusted to 5.8. NAA in the concentration of 13.5 mg·l-1 was efficient for the rooting of microcuttings. An efficient protocol for the reproduction of endemic rowan species by means of organogenesis induction in apical meristems of dormant buds is reported.

Callus-Mediated Organogenesis in <i>Lilium polyphyllum</i> D. Don ex Royle:A Critically Endangered Astavarga Plant

“…In the present study, TDZ had a negative effect on morphogenetic processes in callus explants. Similar findings have been reported for Hypericum species 23 and Agastache rugosa 24 . Hundred per cent rooting was achieved in half-strength MS medium enriched with IBA (9.8 M), and IBA was observed as a potent root inducer in L. polyphyllum compared to IAA and NAA.…”

Diversification and independent polyploid origins in the disjunct species Alyssum repens from the Southeastern Alps and the Carpathians

“…We assume that the distinct position of the Apuseni populations in the present study is caused by their long‐term isolation responding to Quaternary climatic oscillations only by local‐scale migrations in the absence of gene flow to and from adjacent E and SW Romanian lineages. This area of the Apuseni Mts., indeed, harbors isolated, putatively relict populations of several other species of arctic, Alpine or Balkan origin (e.g., Gentiana clusii , Saponaria bellidifolia , Hypericum umbellatum , or Arctostaphylos uva‐ursi ; Csürös and Pop, ; Csergö et al., ; Coste et al., ; Bartok et al., ).…”

Micropropagation of the Narrow Endemic Hladnikia pastinacifolia (Apiaceae)

“…The substrates usually used for acclimatization with other members of that family (Sharma and Wakhlu 2001, Nath and Buragohain 2003, Tavares et al 2010a, Coste et al 2012, Hendrawati et al 2012, Jaheduzzaman et al 2012 were far different from the substrate which promoted acclimatization of our plant species. H. pastinacifolia did not acclimatize at all if the substrates were rich in organic matter (data not shown).…”

“…Root development with an intermediary callus indicated that at that roots and shoots were not well connected with vascular tissue and that plants are insuffi ciently supplied with nutrients (George 1993). Successful rooting in other members of the Apiaceae family was also achieved with 1-5 μM IBA (Tavares et al 2010a, Das et al 2008, Jana and Shekhawat 2011, Coste et al 2012, Jaheduzzaman et al 2012, and with higher concentrations of IBA (Hossain et al 2000, Sharma and Wakhlu 2001, Banerjee et al 1999, Tavares et al 2009-2010, with NAA or IAA (Karuppusamy et al 2007, Jaheduzzaman et al 2012) and only rarely without growth regulators (Makunga et al 2003).…”