In vitro propagation and assessment of genetic stability in date palm as affected by chitosan and thidiazuron combinations

Abstract

Background Mass propagation of date palm has attracted the interest of commercial producers. However, this technique still faces many obstacles that hinder production. This study investigated the effect of chitosan (CHT) at various concentrations for the possibility to apply it in combination with thidiazuron (TDZ) on the growth and development of tissue cultures of Barhee cultivar. Results The results showed that CHT and TDZ on in vitro proliferation of Barhee date palm cultivar were significant. The highest response rate and the number of shoots per jar were found in MS media supplemented with 15 mgL–1 CHT and 0.5 mgL–1 TDZ combination. Furthermore, we found that the combined application between 20 mg L−1 CHT+ 1.0 mg L−1 TDZ resulted in the highest shoots content of endogenous IAA, compared with other treatments. At the same time, the data revealed that the maximum cytokinins (CKs) content of shoots occurred in a medium supplemented with 15 mg L−1 CHT and 0.5 mg L−1 TDZ. The genetic stability of the discussed micropropagation protocol was confirmed in this study by DNA-based technique RAPD (random amplified polymorphic DNA). The results may indicate that the micropropagation protocol developed in this research paper was appropriate and applicable for producing genetically stable date palm cv Barhee plants. Conclusion Applying the strategy of culture treatment with (CHT) and (TDZ) can be valuable for improving the propagation of date palm cv Barhee in vitro.

In vitro assessment for the probiotic potential of Pichia kudriavzevii

“…After 3 hrs of incubation, isolate TS2 exhibited above 50% auto aggregation, which increased to 85% after 20 hrs. A similar result was observed by Lata et al [14] where P kudrivenzii isolated from mango pickle showed 87% auto aggregation after 24 hrs. High hydrophobicity-exhibiting strains have good adhesion properties to intestinal cells.…”

“…The hemolytic activity was determined by inoculating the yeast isolate on blood agar plates containing sheep blood and incubated for 48 h at 30 °C. The development of a clear zone around the colony indicates a positive result [14]. Antibiotic resistance of the yeast isolate was performed by the disc diffusion method using different antibiotic discs such as Nystatin (50µg), Miconazole (10 µg), Clotrimazole (10 µg), Fluconazole (10µg), Ketoconazole (50 µg), Itraconazole (10 µg), Cefmetazole (10 µg) (Sigma Aldrich).The antibiotic discs were placed on yeast-inoculated YPD agar plates and incubated for 48 h at 30 °C, and a zone of inhibition was observed [15].…”

Micropropagation and Acclimatization of Gymnocalycium cv. Fancy (Cactaceae): Developmental Responses to Different Explant Types and Hormone Conditions

“…Given that M3 and M4 are unexpected morphotypes and would hardly arise under traditional sowing conditions, these results suggest that the presence of TDZ in vitro could be acting as an inducer of somaclonal variation, as has been observed in other works [13,86]. Thus, the indirect organogenesis processes that are observed from the calluses could give rise to new morphotypes that would be of commercial interest.…”