In vitro Promotion of Root Formation by Apple Shoots Through Darkness Effect on Endogenous Phenols and Peroxidases

Druart, Philippe; Kevers, Claire; Boxus, P.; Gaspar, Thomas

doi:10.1016/s0044-328x(82)80168-2

Cited by 116 publications

(60 citation statements)

References 10 publications

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“…This pretreatment of the shoots improved roots induction and initiation in several other woody species [27]. Darkness influences the endogenous metabolism of the shoots during the successive phases of the rhizogenesis with, notably, a rapid drop in the activity of endogenous peroxidase and phenolic compounds during the first five days of culture [29]. On the other hand, the use of MS medium increases the C/N ratio by reducing the supply of nitrogen which is favorable to root formation.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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Entire plants were regenerated from nodes explants of Jatropha curcas L. following a procedure of bud aggregate induction on MS (Murashige and Skoog) medium supplemented with 25 mg.l -1 citric acid, 12.2 mg.l -1 adenine sulfate, 15 mg.l -1 L-arginine, 2.46 M IBA (indole-3-butyric acid), 30 g.l -1 sucrose and 7 g.l -1 of agar, and enriched with different balances of BA (benzyladenine) and L-glutamine. The histological studies performed on aggregates showed that the buds result from both the development of axillary buds and adventitious budding starting from underlying tissues of the explant. The culture medium containing 6.65 M BA and 25 mg.l -1 L-glutamine gave the best results with an average of 64 buds per aggregate after three weeks for all accessions tested. The buds developed into shoots when placed in an MS medium supplemented with 2.21 M BA, 5.70 M IAA (indole-3-acetic acid) and 15 mg.l -1 L-arginine. These shoots were isolated and then rooted in MS containing 2.46 M of IBA, 2% sucrose and 0.7% agar. The entire process took 13 weeks with a 98% survival rate in terms of plantlets acclimatization. We obtained a multiplication rate of 13 buds per explant and per subculture which is the double of those obtained in other recent works based on the micropropagation of J. curcas from node explants. This protocol is economically more profitable.

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Section: Discussionmentioning

confidence: 99%

In Vitro Micropropagation of Jatropha curcas L. from Bud Aggregates

Mve

Mergeai

Druart

et al. 2013

J. Technol. Innov. Renew. Energy

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“…Since dedifferentiation Trees (2010) 24:975-992 987 is a part of the regeneration process (Christianson and Warnick 1983), a higher proportion of already undifferentiated cells may improve shoot or root organogenesis. A possible effect of low temperature and darkness could be explained by their influence on auxin metabolism in relation to rooting through modification of peroxidase activities and formation of endogenous phenolic compounds (Druart et al 1982). …”

Section: Temperaturementioning

confidence: 99%

Adventitious rooting of conifers: influence of physical and chemical factors

Ragonezi

Klimaszewska

Castro

et al. 2010

Trees

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In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species.

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“…The explanation of these discrepancies may stem from the fact that in these studies, peroxidase activity was measured in purified extracts and/or not expressed per unit protein. When well evidenced, the (specific) peroxidase activity peak was measured in crude extracts where (poly)phenols apparently modulated the enzyme activity (Druart et al, 1982;Mato et al, 1988;Gaspar et al, 1990;Berthon et al, 1990b). We have further shown (Aghmir et al, 1991) (Nakano et al, 1980;Fouret et al, 1986;Welander and Snygg, 1987;Gaspar and Hofinger, 1988 .…”

mentioning

confidence: 97%

“…This was followed by the rapid emergence of root primordia (Quoirin et al, 1974;Van Hoof and Gaspar, 1976). It was later shown that the elevation and peaking of peroxidase activity preceding rooting might already take place at the end of the foregoing multiplication/elongation cycle (Druart et al, 1982;Hausman et al, 1990).…”

mentioning

confidence: 99%

“…A positive correlation also occurred between the activity of (basic) peroxidases in stem of shoots of apple at the time of transfer to rooting medium and the number of roots formed later (Gus'kov et al, 1988;de Klerk et al, 1990 Benzyladenine used instead of kinetin in the multiplication medium of Asparagus provided a shoot population with a higher peroxidase activity and a higher rooting percentage . Continuous darkness instead of a 16-8 day-night cycle during elongation and root initiative phases of apple shoots favoured successive increase and decrease of peroxidase activity, and resulted in higher percentages of rooted plantlets (Druart et al, 1982). On the contrary, enhancement of peroxidase activity of globe artichoke shoots was favoured by continuous light, whereas a reduced photoperiod or darkness was necessary to achieve the decrease in peroxidase activity and to obtain rooting (Moncousin and Gaspar, 1983 (Gaspar et al, 1977).…”

mentioning

confidence: 99%

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