In-vitro production of TNF-α in blood samples

Freeman, R.; Wheeler, J. W.; Robertson, Helen Lee; Paes, M.L.; Laidler, Judith

doi:10.1016/0140-6736(90)91848-5

Cited by 32 publications

(11 citation statements)

References 8 publications

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“…[44,45] and TNFa in plasma are low and similar in RA patients and healthy controls. The increased serum TNFa concentrations reported in some studies [19,42,46] might have been due to production of TNFa in the blood sample during clotting or to endotoxin contamination [47,48]. Our results corrob orate former findings of an increased spontaneous production [49][50][51][52] and a normal IL-l/i secretion after LPS stimulation [49] in RA.…”

Section: Discussionsupporting

confidence: 78%

Effect of Methotrexate Alone or in Combination With Sulphasalazine on the Production and Circulating Concentrations of Cytokines and Their Antagonists. Longitudinal Evaluation in Patients With Rheumatoid Arthritis

et al. 1995

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S U M M A R YIn a recent study from our group, the combination of methotrexate and sulphasalazine (MTX + SASP) seemed superior to MTX alone in the treatment of rheumatoid arthritis (RA). To assess the impact of these therapies on the cytokine cascade, the in vitro production and circulating concentrations of several cytokines and endogenous cytokine antagonists were measured in 30 healthy controls and longitudinally in a subset of 26 patients enrolled in this study. Compared to controls, RA patients had significantly higher circulating concentrations of interleukin-6 (IL-6), soluble receptors for tumour necrosis factor (sTNFR), soluble receptors for interleukin-2 (sIL-2R) and interleukin-1 receptor antagonists (IL-1RA), and their peripheral blood mononuclear cells (PBMNC) showed a higher spontaneous production of interleukin-1/i (IL-l/i), tumour necrosis factor a (TNFa) and IL-1RA (both secreted and cell-associated) and a higher stimulated production of cell-associated TNFa, IL-1RA and (to a lesser extent) IL-l/i. Treatment with MTX alone (n = 12) or combined with SASP (n = 14), resulted in significant reductions of circulating IL -6 and sIL-2R but did not alter IL-l/i, TNFa or IL-1RA concentrations. Decreases in circulating levels of sTNFR and in the in vitro production of cell-associated IL-l/i and IL-1RA after stimulation were only observed in patients treated with MTX + SASP. The concentrations of IL-1RA and sTNFR in the circulation exceeded moderately those of IL-l/i and TNFa but this is probably insufficient to block IL-1 and TNFa activity. In conclusion, therapy with MTX alone or with SASP modulates IL -6 and sIL-2R concentrations in RA. Decreased production of IL-l/i and IL-1RA and circulating sTNFR levels were only observed during therapy with MTX -f SASP. Whether this relates to the better clinical effect observed with the combination therapy remains to be investigated. Circulating levels of IL-6, sIL-2R and sTNFR seem useful markers of disease activity in RA.K ey w o r d s : Cytokines, Methotrexate, Sulphasalazine, Combination therapy.

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Section: Discussionsupporting

confidence: 78%

Effect of Methotrexate Alone or in Combination With Sulphasalazine on the Production and Circulating Concentrations of Cytokines and Their Antagonists. Longitudinal Evaluation in Patients With Rheumatoid Arthritis

et al. 1995

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“…1 The findings obtained by us with a different technique and in an independent population 2 are in very good agreement with their data and thus support the conclusion drawn by the authors, highlighted in the accompanying summary by Dan Longo. 3 They supplement various other studies in this field, 4-6 confirming our concept of at least 2, if not 3, distinct genetic subgroups.…”

Section: Pathogenic Complexity Of Gastric B-cell Lymphomasupporting

confidence: 66%

“…For example, several investigators have shown that anticoagulants affect leukocyte viability and cause ex vivo changes in cytokine production. 3,4 The extent to which events after blood collection influence gene expression is not known. To our knowledge, changes in gene expression between phlebotomy and the beginning of analysis have not been studied.…”

mentioning

confidence: 99%

Gene expression changes in blood after phlebotomy: implications for gene expression profiling

Pahl

Brune

2002

Blood

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“…In the 48 subjects without the disease (controls), the diagnoses were uterine malformations (10 cases), pelvic adhesions (8), fibroids (6), hydrosalpinx (4), serous cysts (2), der moid cysts (2), tubal intramural occlusion (2), tuberculo sis (1) and normal pelvis (11). A peritoneal fluid sample was not available in 13 cases and 14 controls.…”

Section: Resultsmentioning

confidence: 99%

“…This dis crepancy with previous studies may be due to differences in sample collection procedures. Lipopolysaccharides can contaminate blood-collecting systems, activate mono cytes and stimulate rapid and massive production of tumor necrosis factor a in vitro [11]. This artifactual increase in cytokine synthesis by monocytes can be pre vented by taking samples of biological fluids in calcium chelating agents [12], In our study, blood and peritoneal fluid were collected in EDTA, therefore the tumor necro sis factor a concentrations found should reflect the real in vivo production [13] by circulating and pelvic macro phages.…”

Section: Discussionmentioning

confidence: 99%

Tumor Necrosis Factor in Plasma and Peritoneal Fluid of Women with and without Endometriosis

Vercellini

Benedetti

Rossi³

et al. 1993

Gynecol Obstet Invest

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To determine whether in vivo levels of tumor necrosis factor Α in plasma and peritoneal fluid differ in infertile subjects with and without endometriosis, peripheral blood and peritoneal fluid samples were collected in 94 women undergoing laparoscopy for infertility. Quantitative determinations of tumor necrosis factor Α were performed by an enzyme immunoassay test. Tumor necrosis factor Α levels were below the detection limit (10 pg/ml) in plasma and peritoneal fluid of 42 (91%) and 30 (90%) patients with endometriosis and 40 (83%) and 31(91 %) subjects without the disease, respectively. In our series, plasma and tumor necrosis factor Α levels were not different in infertile women with and without endometriosis.

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In-vitro production of TNF-α in blood samples

Cited by 32 publications

References 8 publications

Effect of Methotrexate Alone or in Combination With Sulphasalazine on the Production and Circulating Concentrations of Cytokines and Their Antagonists. Longitudinal Evaluation in Patients With Rheumatoid Arthritis

Effect of Methotrexate Alone or in Combination With Sulphasalazine on the Production and Circulating Concentrations of Cytokines and Their Antagonists. Longitudinal Evaluation in Patients With Rheumatoid Arthritis

Gene expression changes in blood after phlebotomy: implications for gene expression profiling

Tumor Necrosis Factor in Plasma and Peritoneal Fluid of Women with and without Endometriosis

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