In vitro production of pig embryos: a point of view

Coy, Pilar; Romar, Raquel

doi:10.1071/rd01102

Cited by 60 publications

(23 citation statements)

References 116 publications

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“…It is possible that blastocyst yields from CHX-pretreated oocytes could be further increased by improvements to culture conditions: The development rate of untreated control oocytes was relatively low (10%) in the present study compared with those of other conventional cultures (e.g., 14-23% as reported by Marchal et al [17]). The maturation medium influences subsequent embryo development (e.g., NCSU 23 may be better than Medium 199 [60]), and blastocyst yield is affected by features of the IVF protocol, such as boar identity, availability of frozen sperm, and ratio of medium volume or sperm to the number of oocytes [61][62][63].…”

Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Meiotic Progression and Improvement of Developmental Competence of Pig Oocytes in Vitro by Follicle-Stimulating Hormone and Cycloheximide1

Campbell

Craigon

et al. 2005

Biology of Reproduction

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The effects of FSH, LH, and epidermal growth factor (EGF) on the dynamics of nuclear maturation and subsequent embryo development were examined in pig oocytes cultured either conventionally or after preincubation with cycloheximide (CHX). In conventional culture, FSH or EGF significantly increased the rate of attainment of metaphase II (MII) for both gilt (50.0%+/-4.2% and 54.8%+/-4.3%, respectively; control, 5.8%+/-1.8%; P<0.001) and sow (87.6%+/-3.4% and 78.8%+/-3.9%, respectively; control, 7.8%+/-2.5%; P<0.001) oocytes. Gilt oocytes treated with both FSH and EGF showed an additive response (93.7%+/-2.1%). Treatment with LH had no effect. Preincubation with CHX caused the majority (84-100%) of both gilt and sow oocytes to undergo germinal vesicle breakdown. Compared to those treated with LH and/or EGF (both>80%), fewer FSH-treated oocytes reached metaphase I (43.8%+/-5.3%, P<0.001) by 14 h and MII (48.4%+/-5.9%, P<0.001) by 24 h, although the majority (71%) did mature to MII by 36 h after removal of CHX. After in vitro fertilization, higher proportions of both CHX-pretreated and untreated, FSH-exposed oocytes cleaved (71.3%+/-2.9% and 75.3%+/-3.1%, respectively) compared with those not treated with FSH (37.7%+/-3.0% and 43.0%+/-2.9%, respectively; P<0.001). Pretreatment with CHX significantly increased blastocyst yield for both FSH-treated (32.8%+/-2.0% and 10.3%+/-1.5%, respectively; P<0.001) and untreated (16.7%+/-1.5% and 9.4%+/-1.2%, respectively; P<0.001) oocytes. Polyspermy rates were unaffected. In conclusion, pig oocytes meiotically arrested by CHX before maturation retain and improve their developmental competence. FSH stimulates nuclear maturation but slows meiotic progression.

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Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Meiotic Progression and Improvement of Developmental Competence of Pig Oocytes in Vitro by Follicle-Stimulating Hormone and Cycloheximide1

Campbell

Craigon

et al. 2005

Biology of Reproduction

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“…In porcine, generally, the IVM duration is 36-44 h for COCs followed by being used in in vitro fertilization (IVF), parthenogenetic activation (PA) and SCNT [17] . Limited studies have been carried out to determine the optimal IVM duration up to date.…”

Section: Discussionmentioning

confidence: 99%

“…Among the factors mentioned above, in vitro maturation of oocytes is crucial to the overall efficiency of SCNT [16] . Maturation of oocytes includes nuclear and cytoplasm maturation, and the latter plays a key role in determining developmental potential of pig embryos [17] . Duration of IVM and maturation gas environment are factors that have direct effects on cytoplasm maturation of oocytes.…”

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confidence: 99%

Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer

Cheng

Song²

2006

SCI CHINA SER C

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The increases of H2O2 concentrations in plant cells often occur under biotic and abiotic stress conditions (e.g. light, environmental stresses and plant hormone abscisic acid). Atmospheric H202 as an ancient signal molecule not only plays the key role in inducing evolution of oxygenic photosynthesis, but also modulates many physiological events, such as stomatal movement, hypersensitive responses, programmed cell death and gene expressions. H2O2 levels in cells must sustain a fine equilibrium between production and scavenging. H2O2 enters cells from the apoplast or generated sources, and in turn is distributed in sub-cellular compartments. H202 can modulate the activities of many components in signaling, such as protein phosphatases, protein kinases, transcription factors (TFs), and calcium channels. Elevated cytosolic calcium concentrations will initiate further downstream responses, via the action of calcium-binding proteins. On the other hand, the research of H2O2 as a signal molecule is still in a comparatively juvenile stage, for example, little is known about how the cells sense H2O2, what the rate-limiting steps and most important cellular events are in cell signaling and what kind of genes is specific or necessary to H2O2 signaling. The answers to all the questions depend on the functional genomic and molecular genetics analysis.

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“…Optimal culture conditions ensure the in vitro development of embryos with maximum viability and normal metabolic activity, resulting in cleavage, blastocyst and cell number rates similar to in vivo produced embryos (Coy & Romar, 2002). Optimal culture conditions ensure the in vitro development of embryos with maximum viability and normal metabolic activity, resulting in cleavage, blastocyst and cell number rates similar to in vivo produced embryos (Coy & Romar, 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of culture media on porcine embryos produced by in vitro fertilization or parthenogenetic activation after oocyte maturation with cycloheximide

Marques

Nascimento²,

Gerger³

et al. 2011

Zygote

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This study evaluated the effects of reversible meiotic inhibition and different culture media (PZM3 or NCSU23) on production of porcine embryos by either in vitro fertilization (IVF) or parthenogenetic activation (PA). Oocytes from abattoir-derived ovaries were allocated into two groups for maturation: CHX (5 μg/ml cycloheximide for 10 h) or Control (no CHX). The percentage of metaphase II (MII) oocytes was determined at 36, 40 or 44 h of in vitro maturation. For IVF and PA, denuded oocytes were fertilized with purified sperm for 6 h or activated by electric stimuli. Zygotes were then subdivided into two culture groups: NCSU23 or PZM3. No effect of treatment with CHX and culture media was observed on cleavage (D3) and blastocyst (D7) rates in IVF and PA groups. There are no differences of quality or development rates between IVF-derived embryos cultured in NCSU23 or PZM3. However, we observed high quality PA embryos in PZM3 compared with NCSU23. Maturation arrest with CHX decreased the average blastocyst cell number in IVF while it was increased in PA embryos. As older oocytes are more effectively activated, CHX- blocked oocytes reached the mature stage faster than the control group. In conclusion, the CHX treatment for 10 h, followed by oocyte maturation for 40 h, is an efficient protocol to produce high quality parthenote embryos, especially when they are cultured in PZM3. However, this protocol is not satisfactory for IVF embryos production. In this case, a shorter maturation period could provide better embryo quality.

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In vitro production of pig embryos: a point of view

Cited by 60 publications

References 116 publications

Dynamic Changes in Meiotic Progression and Improvement of Developmental Competence of Pig Oocytes in Vitro by Follicle-Stimulating Hormone and Cycloheximide1

Dynamic Changes in Meiotic Progression and Improvement of Developmental Competence of Pig Oocytes in Vitro by Follicle-Stimulating Hormone and Cycloheximide1

Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer

Effect of culture media on porcine embryos produced by in vitro fertilization or parthenogenetic activation after oocyte maturation with cycloheximide

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