In vitro production of embryos in South American camelids

Trasorras, V.; Giuliano, S.; Miragaya, Marcelo

doi:10.1016/j.anireprosci.2012.10.009

Cited by 18 publications

(18 citation statements)

References 42 publications

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“…Based on the finding by Nowshari, a higher COC RR (94%) is achieved in the dromedary camel when individual follicles are punctured (Nowshari, ). Two favourable techniques laparotomy and transvaginal ultrasound‐guided aspiration can be used to obtain in vivo collection of COCs (Tibary et al., ; Trasorras et al., ). Some studies have reported that the COC recovery methods could influence the number and quality of the COCs (Khatir & Anouassi, ; Tibary et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Trasorras et al. found that 1PB extrusion had occurred in approximately 41% of COCs retrieved from superstimulated South American camelids (Trasorras et al., ). Another study showed that the COCs retrieved from large follicles had more capability for IVP‐derived embryo, because several changes took place in large follicles, which were advantageous not only for nuclear but also for cytoplasmic maturation (Khatir et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…In vitro oocyte maturation is the most critical step for IVP‐derived embryo. Nuclear maturation including 1PB extrusion, cytoplasmic maturation and CC expansion are the three criteria for IVM (Khatir, Anouassi, & Tibary, , ; Trasorras, Giuliano, & Miragaya, ); there is a closed interrelation between these criteria. The diameter of oocyte plays a crucial role in the resumption of meiotic division, which is parallel with follicular size.…”

Section: Introductionmentioning

confidence: 99%

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Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Mesbah

Kafi

Nili

2016

Reprod Domestic Animals

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The morphological and morphometric characteristics of the ovary are fundamental properties for in vitro oocyte maturation. Nuclear maturation, including first polar body (1PB) extrusion, cytoplasmic maturation and cumulus cell (CC) expansion are the criteria for in vitro maturation (IVM) of oocyte. This study was designed to determine the effect of morphological and morphometric features of the ovary on CC expansion and 1PB extrusion during IVM of oocyte in the adult female dromedary camel. The weight, volume and three dimensions of ovaries from slaughtered dromedary camels and oocytes inside zona diameter and zona pellucida thickness were measured. The follicles were classified in regard to the size and oocytes according to their ooplasm appearance and CC compactness. Aspirated cumulus oocyte complexes (COCs) were incubated for 48 hr (with a 6-hr interval) in Hams-F10, and CC expansion and 1PB extrusion were assessed. Significant differences were seen in the shape, weight, volume and three dimensions of the ovaries between ≤4-year-old and >4-year-old dromedary camel (p < .5). Approximately, 95.82% of follicles were 2-4 mm in diameter. The mean (±SD) of inside zona diameter of the oocyte and zona pellucida thickness was 132.22 ± 13.8 and 14.64 ± 2.24 μm, respectively, in >4-year-old dromedary camel. The CC expansion and 1PB extrusion were seen in 86% and 21.88% of COCs, respectively. Age and sexual conditions of dromedary camel influence the morphological and morphometric characteristics of the ovary. Most COCs retrieved from 2-6 mm follicles are cultivable. The most slaughterhouse-derived COCs retrieved from 2-6 mm follicles of non-pregnant dromedary camels are excellent and good and yielding a most favourable diameter to achieve the developmental competence for IVM in an optimal time of 24-30 hr; the optimal time for CC expansion is 24-30 hr in this species. However, the CC expansion is a prerequisite process, but not sufficient for IVM.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Mesbah

Kafi

Nili

2016

Reprod Domestic Animals

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“…Nonetheless, there does appear to be a consensus that llama oocytes from slaughterhouse ovaries need approximately 36 h in culture to mature to the MII stage ( 11 , 23 ), and in the case of alpacas, 32 h resulted in the highest rate of oocytes reaching the MII stage ( 12 ). Furthermore, as oocytes were recovered from an abattoir, it is unknown if the dominant follicles were growing or regressing, a fact that would affect oocyte quality ( 24 ).…”

Section: In Vitro Embryo Productionmentioning

confidence: 99%

Production, Preservation, and Transfer of South American Camelid Embryos

et al. 2017

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The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection. All these techniques also require morphologically normal, motile spermatozoa to achieve fertilization. Methods used to decrease semen viscosity and to select the best spermatozoa (Percoll®; Androcoll-ETM) are described. Additionally, nuclear transfer or cloning has been applied in llamas. Up to now, embryo deep-freezing and vitrification have progressed slowly but are at the height of development. Embryos that are obtained by any of these techniques, either in vivo or in vitro, need to be transferred to synchronized recipient females. The best results are achieved after transfer to the left uterine horn with an ipsilateral ovulation. No live offspring have been obtained after the transfer of cryopreserved embryos. Applying reproductive biotechnologies, such as those described, will permit the expansion of genetically selected animals in the population and also that of wild camelid species, vicunas, and guanacos, whose embryos could then be transferred to the uterus of domestic species.

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“…For these reasons, past research has been mainly focused on reproductive mechanisms (Bravo et al, ; Correa et al, ; Ratto et al, , , ; Ruiz et al, ). The study of early morphogenesis in Vicugna pacos became more important after the introduction of reproductive techniques such as in vitro embryo production (Trasorras et al, ). However, not much is known of the neuroanatomy of this species during the elongation phase of embryogenesis and subsequent fetal development.…”

mentioning

confidence: 99%

Mapping of the Early Intrauterine Morphogenesis in the Alpaca (Vicugna pacos): External Features and Development of the Cephalic Vesicle in Comparison with the Progressive Carnegie Scale

Montelli

Stelletta

Ruiz

et al. 2018

The Anatomical Record

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In this study, we characterized the morphological aspects of the early development of the head of the alpaca (Vicugna pacos) and identified the main structures of the central nervous system during the first trimester of pregnancy. The topography and the cytoarchitecture of the fetal brain regions were described by histological analysis of the brain sections. We performed this analysis on alpaca embryos and fetuses presumably aged 20, 30, 45, and 90 days. For the description of the external body structures we considered the shape of the head, the development of the optic primordium, the dorsal curvature of the body, the limb buds, the umbilical cord and relative vessels, and the thickness and transparency of the skin. The prosencephalic, mesencephalic, and the rhomboencephalic vesicles were described by analyzing sagittal sections of the head. The present article provides the first progressive morphological and anatomical description of alpaca brain during early development. A detailed study represents an important basis to further understand the phases of prenatal development in this species, since information about alpaca embryology in incomplete and reproductive failure is a relevant factor. These data are important also for interspecies comparisons and application of reproductive biotechnologies. Anat Rec, 302:1226–1237, 2019. © 2018 Wiley Periodicals, Inc.

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In vitro production of embryos in South American camelids

Cited by 18 publications

References 42 publications

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Cumulus cell expansion and first polar body extrusion during in vitro oocyte maturation in relation to morphological and morphometric characteristics of the dromedary camel ovary

Production, Preservation, and Transfer of South American Camelid Embryos

Mapping of the Early Intrauterine Morphogenesis in the Alpaca (Vicugna pacos): External Features and Development of the Cephalic Vesicle in Comparison with the Progressive Carnegie Scale

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