In vitro production and initiation of pregnancies in inter-genus nuclear transfer embryos derived from leopard cat (Prionailurus bengalensis) nuclei fused with domestic cat (Felis silverstris catus) enucleated oocytes

Yin, Xi-Jun; Lee, Young-Ho; Lee, Hyosang; Kim, Namhyong; Kim, Lyoung-Ho; Shin, Hyoung-Doo; Kong, Il‐Keun

doi:10.1016/j.theriogenology.2005.11.016

Cited by 38 publications

(23 citation statements)

References 14 publications

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“…Cleavage was not affected by the origin of the donor cell and was comparable with results previously reported in other felid species (Hwang et al 2001, Gó mez et al 2004b, Lorthongpanich et al 2004, Yin et al 2006, Wang et al 2009a. Moreover, it was enhanced by embryo aggregation as the rates of cleaved embryos improved nearly 10% in both aggregated groups when compared with their non aggregated counterparts.…”

Section: Effect Of Iscnt and Aggregation On In Vitro Development Of Dsupporting

confidence: 88%

“…This approach becomes relevant in those species for which oocytes are very difficult to obtain. The successful application of iSCNT in felids was demonstrated by the birth of African wild cats (Felis silvestris lybica) (Gó mez et al 2004b) and sand cats (Felis margarita) (Gó mez et al 2008), as well as by pregnancies reported from embryos generated by the fusion of leopard cat (Prionailurus bengalensis) cells with Dc enucleated oocytes (Yin et al 2006). Despite these achievements, pregnancies and births after iSCNT are still elusive, as shown in several reports (Thongphakdee et al 2010, Gó mez et al 2011, Imsoonthornruksa et al 2012.…”

Section: Introductionmentioning

confidence: 99%

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Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. Dc oocytes were in vitro matured and subjected to zona pellucida free (ZP-free) SCNT or iSCNT, depending on whether the nucleus donor cell was of Dc or Ch respectively. ZP-free reconstructed embryos were then cultured in microwells individually (Dc1X and Ch1X groups) or in couples (Dc2X and Ch2X groups). Embryo aggregation improved in vitro development obtaining 27.4, 47.7, 16.7 and 28.3% of blastocyst rates in the Dc1X, Dc2X, Ch1X and Ch2X groups, respectively (P!0.05). Moreover, aggregation improved the morphological quality of blastocysts from the Dc2X over the Dc1X group. Gene expression analysis revealed that Ch1X and Ch2X blastocysts had significantly lower relative expression of OCT4, CDX2 and NANOG than the Dc1X, Dc2X and IVF control groups. The OCT4, NANOG, SOX2 and CDX2 genes were overexpressed in Dc1X blastocysts, but the relative expression of these four genes decreased in the Dc2X, reaching similar relative levels to those of Dc IVF blastocysts. In conclusion, Ch blastocysts were produced using Dc oocytes, but with lower relative expression of pluripotent and trophoblastic genes, indicating that nuclear reprogramming could be still incomplete. Despite this, embryo aggregation improved the development of Ch and Dc embryos, and normalized Dc gene expression, which suggests that this strategy could improve full-term developmental efficiency of cat and feline iSCNT embryos.Reproduction (2015) 150 1-10

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Section: Effect Of Iscnt and Aggregation On In Vitro Development Of Dsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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“…Our results were not different from several other recent reports (Chung et al 2009, Lagutina et al 2010, who had also reported that iSCNT embryos failed to reactivate the embryonic genome and arrested at the stage of EGA specific to the recipient oocyte. Conversely, inter-genus (Oh et al 2006, Yin et al 2006, inter-family (Dominko et al 1999, Zhao et al 2007, inter-order (Yang et al 2003, Wen et al 2005, Illmensee et al 2006, and even inter-class iSCNT (Chen et al 2002, Liu et al 2004 nuclear transfer embryos were reported to have Pig-mouse cytoplasmic hybrid embryos developed to the blastocyst stage. However, these later reports also observed that the majority of iSCNT embryos arrested around the stage of EGA and only a very small percent of embryos developed to blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

Nuclear–cytoplasmic incompatibility and inefficient development of pig–mouse cytoplasmic hybrid embryos

Amarnath

Choi²,

Moawad³

et al. 2011

Reproduction

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Inter-species somatic cell nuclear transfer (iSCNT) embryos usually fail to develop to the blastocyst stage and beyond due to incomplete reprogramming of donor cell. We evaluated whether using a karyoplast that would require less extensive reprogramming such as an embryonic blastomere or the meiotic spindle from metaphase II oocytes would provide additional insight into the development of iSCNT embryos. Our results showed that karyoplasts of embryonic or oocyte origin are no different from somatic cells; all iSCNT embryos, irrespective of karyoplast origin, were arrested during early development. We hypothesized that nuclear-cytoplasmic incompatibility could be another reason for failure of embryonic development from iSCNT. We used pig-mouse cytoplasmic hybrids as a model to address nuclear-cytoplasmic incompatibility in iSCNT embryos. Fertilized murine zygotes were reconstructed by fusing with porcine cytoplasts of varying cytoplasmic volumes (1/10 (small) and 1/5 (large) total volume of mouse zygote). The presence of pig cytoplasm significantly reduced the development of mouse zygotes to the blastocyst stage compared with control embryos at 120 h post-human chorionic gondotropin (41 vs 6 vs 94%, P!0.05; 1/10, 1/5, control respectively). While mitochondrial DNA copy numbers remained relatively unchanged, expression of several important genes namely Tfam, Polg, Polg2, Mfn2, Slc2a3 (Glut3), Slc2a1 (Glut1), Bcl2, Hspb1, Pou5f1 (Oct4), Nanog, Cdx2, Gata3, Tcfap2c, mt-Cox1 and mt-Cox2 was significantly reduced in cytoplasmic hybrids compared with control embryos. These results demonstrate that the presence of even a small amount of porcine cytoplasm is detrimental to murine embryo development and suggest that a range of factors are likely to contribute to the failure of inter-species nuclear transfer embryos.

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“…Although the remaining population of the MC is not known, the International Union for the Conservation of Nature and Natural Resources (IUCN) classifies the MC as intermediate and the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) has listed the MC as endangered in Appendix I. Since endangered species have a limited supply of recipient oocytes, the oocyte cytoplasm of domestic species, such as the bovine, sheep, rabbit, DC and dog, has been used for dedifferentiation of the somatic cell nucleus from the rat, pig, monkey, sheep [11], gaur [12], buffalo [13], giant panda [14], banteng [15], wolf [16], goat [17] monkey [18], human [19,20] and several felid species [6][7][8][9][10]21]. Gomez et al [10] suggested that DC oocytes can be used for wildcat preservation by interspecies or intergeneric NT techniques.…”

mentioning

confidence: 99%

The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

Imsoonthornruksa

Lorthongpanich

Sangmalee

et al. 2011

Journal of Reproduction and Development

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Abstract. The aim of this study was to investigate if reconstructed felid embryos obtained by intraspecies or intergeneric cloning can develop in vitro. Fibroblast cells (f) from a domestic cat (DCf), marbled cat (MCf) and bovine (Bf) were used as donor cells, and oocytes (o) from domestic cats (DCo) and bovine (Bo) were used as recipient cytoplasts. There were two intraspecies (donor cell + recipient cytoplast: DCf + DCo and Bf + Bo) and three intergeneric (MCf + DCo, DCf + Bo and MCf + Bo) cloning groups in the study. In Experiment 1, the effects of manipulation media, modified or Emcare holding medium (EHM), on in vitro development of DCf + DCo embryos were investigated. The blastocyst formation rate (BFR) of the embryos manipulated in EHM (33.3%) was higher (P<0.05) compared with those manipulated in 199H (18.1%). In Experiment 2, DCf + DCo and MCf + DCo embryos were cocultured with or without domestic cat oviductal epithelium cells. Irrespective of coculture, the same BFR was obtained for DCf + DCo embryos (44.4 vs. 38.0%), while MCf + DCo embryos could not develop beyond the morula stage. In experiment 3, although the development of MCf + DCo and DCf + Bo embryos was arrested at the morula stage, 8.6% of MCf + Bo embryos were able to develop to the blastocyst stage. These results demonstrated that EHM was superior to 199H as an embryo manipulation medium and that the DCo and Bo could support the early embryonic development of intergeneric cloned marbled cat embryos up to the morula stage. However, postimplantation development still needs to be investigated. Key words: Bovine cytoplast, Development, Felid embryos, Intergeneric cloning, Marbled cat (J. Reprod. Dev. 57: [385][386][387][388][389][390][391][392] 2011) is the world's first domestic cat (DC) cloned using an adult somatic cell as a donor nucleus [1]. To date, several reports have successfully documented domestic kittens [2][3][4][5] cloned by NT. There are many efforts currently underway to study the conservation of endangered felid species. In order to preserve and increase the numbers of endangered felid species such as the African wildcat [6,7], leopard cat [3, 8], marbled cat (MC; Pardofelis marmorata) [9] and sand cat [10], nuclear transfer (NT) has been used to produce cloned embryos.Interspecies or intergeneric NT is a high potential technique for conserving a number of endangered species. One such species is the MC, a wildcat that is considered at risk of extinction in Southeast Asia. The size of the MC is larger than a big DC, and it has a base fur color that ranges from brownish to grey with a pattern of irregular dark marbled blotches and spots outlined with black strips. The declining population could be attributed to poaching and human destruction of the natural ecosystem. Although the remaining population of the MC is not known, the International Union for the Conservation of Nature and Natural Resources (IUCN) classifies the MC as intermediate and the Convention on International Trade in Endangered Species of Wild Fauna and Flo...

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In vitro production and initiation of pregnancies in inter-genus nuclear transfer embryos derived from leopard cat (Prionailurus bengalensis) nuclei fused with domestic cat (Felis silverstris catus) enucleated oocytes

Cited by 38 publications

References 14 publications

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Nuclear–cytoplasmic incompatibility and inefficient development of pig–mouse cytoplasmic hybrid embryos

The Effects of Manipulation Medium, Culture System and Recipient Cytoplast on In Vitro Development of Intraspecies and Intergeneric Felid Embryos

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