In vitro postovulatory oocyte aging affects H3K9 trimethylation in two-cell embryos after IVF

Petri, Theresa; Dankert, Debora; Demond, Hannah; Wennemuth, Gunther; Horsthemke, Bernhard; Grümmer, Ruth

doi:10.1016/j.aanat.2019.151424

Cited by 8 publications

(4 citation statements)

References 64 publications

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“…In the last decades, various groups have evidenced that POA mechanisms can interfere with the normal epigenetic asset before and after fertilization, thus affecting embryo epigenome and its further development [74,86]. In vivo and in vitro studies examined DNA methylation among the epigenetic modifications and found a POA-dependent loss of methylation in the CpG of maternal imprinting genes Snrpn and Peg1/Mest after 13 h of culture and 29-h post-hCG injection [87,88].…”

Section: Epigenomic and Genomic Aberrations In Postovulatory Agingmentioning

confidence: 99%

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Nisio

Antonouli

Damdimopoulou

et al. 2022

J Assist Reprod Genet

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In mammalian species an optimal fertilization window during which successful fertilization occurs. In the majority of mammals estrus marks ovulation time and coincident with mating, thereby allowing the synchronized meeting in the fallopian tubes, between freshly ejaculated sperm and freshly ovulated oocytes. Conversely, women do not show natural visual signs of ovulation such that fertilization can occur hours later involving an aged oocyte and freshly ejaculated spermatozoa. During this time, the oocyte undergoes a rapid degradation known as “postovulatory aging” (POA). POA may become particularly important in the human-assisted reproductive technologies, as the fertilization of retrieved mature oocytes can be delayed due to increased laboratory workload or because of unforeseeable circumstances, like the delayed availability of semen samples. This paper is an updated review of the consequences of POA, either in vivo or in vitro, on oocyte quality with particular attention to modifications caused by POA on oocyte nuclear, cytoplasmic, genomic, and epigenetic maturation, and embryo development.

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Section: Epigenomic and Genomic Aberrations In Postovulatory Agingmentioning

confidence: 99%

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Nisio

Antonouli

Damdimopoulou

et al. 2022

J Assist Reprod Genet

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“…Although being in different stages of the menstrual cycle has a certain impact on reproduction, we inferred that this result was caused by the abnormal development of follicles and oocytes after Kdm2a deletion. Folliculogenesis and oogenesis involve highly dynamic changes in morphogenesis, chromatin structure, and gene transcription [ 31 , 32 ], and these events in an orderly manner lead to matured oocyte ovulation and are necessary to regulate embryo development [ 33 ]. This entire process is regulated by numerous factors, including epigenetic modification.…”

Section: Discussionmentioning

confidence: 99%

Oocyte-Specific Knockout of Histone Lysine Demethylase KDM2a Compromises Fertility by Blocking the Development of Follicles and Oocytes

Xiong

Zhang

Yang

et al. 2022

IJMS

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The methylation status of histones plays a crucial role in many cellular processes, including follicular and oocyte development. Lysine-specific demethylase 2a (KDM2a) has been reported to be closely associated with gametogenesis and reproductive performance, but the specific function and regulatory mechanism have been poorly characterized in vivo. We found KDM2a to be highly expressed in growing follicles and oocytes of mice in this study. To elucidate the physiological role of Kdm2a, the zona pellucida 3-Cre (Zp3-Cre)/LoxP system was used to generate an oocyte Kdm2a conditional knockout (Zp3-Cre; Kdm2aflox/flox, termed Kdm2a cKO) model. Our results showed that the number of pups was reduced by approximately 50% in adult Kdm2a cKO female mice mating with wildtype males than that of the control (Kdm2aflox/flox) group. To analyze the potential causes, the ovaries of Kdm2a cKO mice were subjected to histological examination, and results indicated an obvious difference in follicular development between Kdm2a cKO and control female mice and partial arrest at the primary antral follicle stage. The GVBD and matured rates of oocytes were also compromised after conditional knockout Kdm2a, and the morphological abnormal oocytes increased. Furthermore, the level of 17β-estradiol of Kdm2a cKO mice was only 60% of that in the counterparts, and hormone sensitivity decreased as the total number of ovulated and matured oocytes decreased after superovulation. After deletion of Kdm2a, the patterns of H3K36me2/3 in GVBD-stage oocytes were remarkedly changed. Transcriptome sequencing showed that the mRNA expression profiles in Kdm2a cKO oocytes were significantly different, and numerous differentially expressed genes were involved in pathways regulating follicular and oocyte development. Taken together, these results indicated that the oocyte-specific knockout Kdm2a gene led to female subfertility, suggesting the crucial role of Kdm2a in epigenetic modification and follicular and oocyte development.

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“…The competence of the oocyte to fertilise and develop into a normal embryo declines with increased post-ovulatory oocyte age that begins at ovulation and progresses constantly until fertilisation [ 1 ]. The mechanisms driving the oocyte aging process are not yet clear.…”

Section: Discussionmentioning

confidence: 99%

“…High-quality eggs are essential for proper embryo development and good health in the later life of the offspring [ 1 ]. Internal components such as maternal proteins or gene transcripts and external factors such as brood stock diet, environmental conditions, husbandry practices, and oocyte aging can influence the quality of oocytes [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio

Waghmare

Samarin

Danielsen

et al. 2021

IJMS

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Aging is the most critical factor that influences the quality of post-ovulatory oocytes. Age-related molecular pathways remain poorly understood in fish oocytes. In this study, we examined the effect of oocyte aging on specific histone acetylation in common carp Cyprinus carpio. The capacity to progress to the larval stage in oocytes that were aged for 28 h in vivo and in vitro was evaluated. Global histone modifications and specific histone acetylation (H3K9ac, H3K14ac, H4K5ac, H4K8ac, H4K12ac, and H4K16ac) were investigated during oocyte aging. Furthermore, the activity of histone acetyltransferase (HAT) was assessed in fresh and aged oocytes. Global histone modifications did not exhibit significant alterations during 8 h of oocyte aging. Among the selected modifications, H4K12ac increased significantly at 28 h post-stripping (HPS). Although not significantly different, HAT activity exhibited an upward trend during oocyte aging. Results of our current study indicate that aging of common carp oocytes for 12 h results in complete loss of egg viability rates without any consequence in global and specific histone modifications. However, aging oocytes for 28 h led to increased H4K12ac. Thus, histone acetylation modification as a crucial epigenetic mediator may be associated with age-related defects, particularly in oocytes of a more advanced age.

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In vitro postovulatory oocyte aging affects H3K9 trimethylation in two-cell embryos after IVF

Cited by 8 publications

References 64 publications

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Oocyte-Specific Knockout of Histone Lysine Demethylase KDM2a Compromises Fertility by Blocking the Development of Follicles and Oocytes

Histone Acetylation Dynamics during In Vivo and In Vitro Oocyte Aging in Common Carp Cyprinus carpio

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