In vitro post-meiotic germ cell development from human embryonic stem cells

Aflatoonian, Behrouz; Ruban, Ludmila; Jones, Mark; Aflatoonian, Reza; Fazeli, Alireza; Moore, H. D. M.

doi:10.1093/humrep/dep334

Cited by 128 publications

(117 citation statements)

References 33 publications

Supporting

Mentioning

107

Contrasting

Unclassified

Order By: Relevance

“…The efficiency of differentiation from ES or iPS cells into haploid male germ cells is generally reported to be low. [14][15][16][17][18][19][20][21][22][23][24] Although there is no evidence for the speculation that this efficiency might be higher using AFS cells, to our opinion, the here reviewed knowledge warrants testing this new stem cell type for their usability for the in vitro investigation of sperm production (Figure 1).…”

Section: Amniotic Fluid Stem (Afs) Cells Could Be a Useful Tool To Stmentioning

confidence: 89%

Amniotic fluid stem cell-based models to study the effects of gene mutations and toxicants on male germ cell formation

Gundacker¹,

Dolznig²,

Mikula³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

Male infertility is a major public health issue predominantly caused by defects in germ cell development. In the past, studies on the genetic regulation of spermatogenesis as well as on negative environmental impacts have been hampered by the fact that human germ cell development is intractable to direct analysis in vivo. Compared with model organisms including mice, there are fundamental differences in the molecular processes of human germ cell development. Therefore, an in vitro model mimicking human sperm formation would be an extremely valuable research tool. In the recent past, both human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells have been reported to harbour the potential to differentiate into primordial germ cells and gametes. We here discuss the possibility to use human amniotic fluid stem (AFS) cells as a biological model. Since their discovery in 2003, AFS cells have been characterized to differentiate into cells of all three germ layers, to be genomically stable, to have a high proliferative potential and to be non-tumourigenic. In addition, AFS cells are not subject of ethical concerns. In contrast to iPS cells, AFSs cells do not need ectopic induction of pluripotency, which is often associated with only imperfectly cleared epigenetic memory of the source cells. Since AFS cells can be derived from amniocentesis with disease-causing mutations and can be transfected with high efficiency, they could be used in probing gene functions for spermatogenesis and in screening for male reproductive toxicity.

show abstract

Section: Amniotic Fluid Stem (Afs) Cells Could Be a Useful Tool To Stmentioning

confidence: 89%

Amniotic fluid stem cell-based models to study the effects of gene mutations and toxicants on male germ cell formation

Gundacker¹,

Dolznig²,

Mikula³

et al. 2012

Asian J Androl

View full text Add to dashboard Cite

show abstract

“…This study instigated the necessity to identify growth factors and culture strategies to obtain true PGCs and gonocytes in vitro. Follow-up studies unravelled the important growth factors and highlighted the need for dose response studies and the implementation of step-wise differentiation protocols (Kee et al 2006;Tilgner et al 2008;Aflatoonian et al 2009;Kee et al 2009;Park et al 2009;Tilgner et al 2010;Eguizabal et al 2011;Chuang et al 2012;Easley et al 2012). BMP4, similar to its role in inducing PGC-directed differentiation of mESCs, was found to be a key player in differentiation of hESCs towards the germ cell lineage (Kee et al 2006).…”

Section: Embryonic Stem Cells Derived Germ Cells and Gametesmentioning

confidence: 99%

Use of pluripotent stem cells for reproductive medicine: are we there yet?

Duggal

Heindryckx

Deroo

et al. 2014

Veterinary Quarterly

View full text Add to dashboard Cite

In recent years, pluripotent stem cells have demonstrated to be exciting tools to understand embryonic development, cell lineage specification, tissue generation and repair, and various other biological processes. In addition, the identification and isolation of germ line stem cells has given more insight into germ cell biology at the molecular level and into the underlying causes of infertility which was not possible earlier. The recent derivation of in vitro derived sperm and oocytes from pluripotent stem cells in the mouse model represents a major breakthrough in the field and substantiates the critical relevance of stem cells as a potential alternative resource for treating infertility. Although the past years have yielded compelling information in understanding germ cell development via in vitro stem cell assays, extended investigative research is necessary in order to derive fully functional 'artificial gametes' in a safe way for future therapeutic applications.

show abstract

“…Certains marqueurs commencent à être reconnus comme incontournables pour l'établissement du phénotype des CGP : ainsi PRDM1, sous le contrôle de la signalisation BMP/SMAD, est essentiel à l'orientation des cellules CSE vers une identité CGP chez la souris comme chez l'homme. Chez l'humain, deux équipes ont mis en évidence les transcrits PRDM1 dans des CGP obtenues à partir de hCSE par différents protocoles qui seront détaillés ci-après [7,8] TRA-1-60 [7,16,22] TRA-1-81…”

Section: Obtention De Cellules Germinales Primordiales Et De Gonocyteunclassified

“…[5, 7,13,16,19,22,24,25,27] Cellules Sertoli, cellules Leydig, cellules de la granulosa, cellules de la thèque…”

Section: V-kit Hardy-zuckerman 4 Feline Sarcoma Viral Oncogene Homologunclassified

“…Ainsi, les tentatives de différenciation de cellules souches pluripotentes en gamètes mâles font appel à une sélection de déterminants moléculaires précis. L'haploïdisation des cellules a été rapportée dans quatre études [8,16,17,19] mais sur un nombre restreint de cellules. La différenciation mâle obtenue dans l'ensemble de ces études n'est pas totalement complète (morphologie peu décrite), et la fonctionnalité des cellules germinales obtenues n'a pas encore été testée chez l'humain.…”

Section: Revuesunclassified

See 1 more Smart Citation