In vitro positron emission tomography (PET): use of positron emission tracers in functional imaging in living brain slices

The Nature of Dopamine Dysfunction in Schizophrenia and What This Means for Treatment

“…[22][23][24][25] We group findings into studies of presynaptic dopaminergic function (dopamine synthesis capacity, dopamine release, and synaptic dopamine levels), dopamine transporter availability, and dopamine receptor availability. The studies of dopamine synthesis capacity are grouped with those of dopamine release and synaptic dopamine levels (which use pharmacological challenges that either deplete or release dopamine from presynaptic terminals) because animal [26][27][28] and in vivo human evidence 29 indicates that they index related aspects of dopaminergic function. However, the results are also given separately for these different methodological approaches for comparison.…”

In Vitro Imaging Techniques in Neurodegenerative Diseases

“…The advantages of cultured organ slices are, for example, the maintained cellular organization and cellular interactions and the possibility for more precise manipulation of the extracellular environment and for using medium-term incubation time (hours to a few days depending on the target system studied) [42]. Matsumura et al [38] used cultured brain slices in combination with positronemitting radionuclides to observe perturbation of glucose metabolism ([ 18 F]FDG), D 2 dopamine receptor binding ([ 11 C]raclopride), neurotransmitter precursor uptake, and neurotransmitter release (L-[ 11 C]DOPA) [38].…”

Quantitative in vitro phosphor imaging using [3H] and [18F] radioligands: the effects of chronic desipramine treatment on serotonin 5-HT2 receptors

“…Besret et al, 1998;Passchier et al, 2000;Doze et al, 2002). Phosphor imaging with PET tracers has also been used with live tissue preparations, where the tissue is removed from the animal but maintained in biological buffer solutions with oxygenation during incubation with the PET compound (Matsumura et al, 1995;Sasaki et al, 2002). Very few studies have used in vitro phosphor imaging with PET tracers for quantitative autoradiography (Gatley et al, 1998;Nikolaus et al, 2001Nikolaus et al, , 2003.…”