In vitro plantlet regeneration of Schinopsis balansae (Anacardiaceae)

Sansberro, Pedro Alfonso; Rey, H. Y.; Mroginski, L. A.; Luna, Claudia Verónica

doi:10.1007/s00468-003-0271-1

Cited by 9 publications

(5 citation statements)

References 22 publications

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“…The promotive role of BA for shoot differentiation has been documented in Cercis canadensis var. mexicana (Mackay et al 1995), Schinopsis balansae (Sansberro et al 2003), and Holarrhena antidysenterica (Kumar et al 2005). However, shoot growth was best on the basal medium and the lowest level of BA tested (Table 1, Fig.…”

Section: Resultsmentioning

confidence: 98%

“…Auxins are widely used for induction of roots on regenerated shoots. IBA was effective for development of shoots in various members of the Anarcardiaceae (Boggetti et al 1999;Sansberro et al 2003). These plantlets were transferred to small pots containing garden soil: vermiculite (1:1) and kept in a mist house for 14 d. After the development of new leaves, plants were moved to a greenhouse.…”

Section: Resultsmentioning

confidence: 99%

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Micropropagation of Searsia dentata

Prakash

Staden

2008

In Vitro Cell.Dev.Biol.-Plant

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A method for in vitro regeneration of Searsia dentata from nodal and shoot tip explants derived from mature trees is outlined. Nodal explants produced multiple shoots from the axis when cultured on Murashige and Skoog (MS) medium containing 3% sucrose supplemented with 0, 5, 7.5, 10, or 12.5 μM N 6 -benzyladenine (BA). An average of 5.3 shoots was obtained from nodal explants on 10 μM BA. For shoot tip explants, however, supplementation of !-naphthaleneacetic acid (NAA) with BA favored a caulogenic response. A maximum of 6.1 shoots were produced per shoot tip explant on MS containing 7.5 μM BA plus 5.0 μM NAA. The in vitro-regenerated shoots produced roots when transferred to full-strength MS medium containing 3% sucrose and 10 μM indole-3-butyric acid (IBA). The developed plantlets were transferred initially to a mist house. After an initial acclimatization period of 3-4 mo, plantlets were shifted to the greenhouse where they thrived for 9 mo. The standardized protocol for mass propagation of S. dentata should eliminate the dependence on natural stands of plants for traditional medicinal purposes, and will also serve as a means of conservation as the species is heavily overexploited.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Micropropagation of Searsia dentata

Prakash

Staden

2008

In Vitro Cell.Dev.Biol.-Plant

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“…On the contrary, in the presence of PVP, the release of phenolics was increased resulting in reduction of sprouting frequency. Growth-enhancing effect of charcoal has been reported in tree species of Anacardiaceae in vitro, such as in Schinopsis balansae (Sansberro et al 2003) and cashewnut (Das et al 1996). The phenolics released by the tissues in subculture often inhibit the growth and morphogenesis (Onay 2003).…”

Section: Influence Of Antioxidantsmentioning

confidence: 96%

In vitro regeneration of Semecarpus anacardium L. from axenic seedling-derived nodal explants

Panda

Hazra

2010

Trees

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Semecarpus anacardium (Anacardiaceae), a deciduous forest tree, is a potent source of medicinal compounds. Poor seed viability of this species limits the conventional propagation practice. Proliferation of shoots from axillary meristem was achieved in semisolid WPM medium supplemented with BAP 4.44 lM and KN 4.64 lM. Factors including culture vessels, gelling agents and antioxidants were identified and optimized for proliferation and growth of shoots in vitro. Cotton-plugged culture vessels were more favorable. Phytagel 0.2% as gelling agent and activated charcoal 0.2% as antioxidant were superior to other agents and antioxidants tested. All the shoots rooted in half-strength WPM liquid medium with IBA 2.46 lM. Rooted shoots survived (91%) in the soil-sand 1:1 mixture. Ex vitro rooting of shoots and hardening of plants were achieved in 80% of the explants in the soil-sand mixture. Hardened plants were maintained in a greenhouse. This is the first report on in vitro regeneration of Semecarpus anacardium.

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“…In the present study, the maximum promotive effect on percent bud-break and rate of shoot multiplication were observed with BA as compared to 2-iP or Kinetin. The promotive role of BA on shoot proliferation has been previously documented in Schinopsis balansae (Sansberro et al 2003), Holarrhena antidysenterica (Kumar et al 2005), Eclipta alba (Ray and Bhattacharya 2008), Nelumbo nucifera (Shou et al 2008), and Centaurea ultreiae (Mallón et al 2010). In the present work, 6.4-fold shoot multiplication was obtained in 64% cultures on hormone-free medium.…”

Section: Discussionmentioning

confidence: 91%

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

Singh

Chaturvedi

2010

Plant Cell Tiss Organ Cult

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A reproducible protocol for clonal propagation of Spilanthes acmella has been established. Routinely, the cultures were established in spring (January-April) season because of the highest aseptic culture establishment and high frequency shoot proliferation. Incorporation of 5 lM N 6 -benzyladenine (BA) to Murashige and Skoog (MS) basal medium showed 100% bud-break and promoted multiple shoot proliferation in cultures. Interestingly, a higher concentration of BA (7-15 lM) promoted stunted shoots with pale leaves while a lower concentration (1-3 lM) resulted in shoots with long internodes and excessive adventitious root proliferation from all over their surface. For recurrent shoot multiplication, single node segments from in vitro-developed shoots were excised and cultured on MS ? BA (5 lM) medium where 20.3-fold shoot multiplication was achieved every 5 weeks. Finally, these shoots were successfully rooted on half-strength MS medium (major salts reduced to half-strength) with 50 g l -1 sucrose, with a frequency of 100%. Transplantation survival of micropropagated plants was 88.9%. Additionally, accumulation of scopoletin, a phytoalexin, was revealed for the first time in the uninfected leaves of Spilanthes. Further, the quantitative estimation by HPLC with a fluorescence detector showed that the amounts of scopoletin content (0.10 lg g -1 DW) in the leaves of micropropagated plants are comparable to those of field-grown mother plants. The study thus signifies the effectiveness of in vitro methodology for true-to-type plant regeneration of Spilanthes and their later utility for biosynthesis and constant production of scopoletin throughout the year.

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In vitro plantlet regeneration of Schinopsis balansae (Anacardiaceae)

Cited by 9 publications

References 22 publications

Micropropagation of Searsia dentata

Micropropagation of Searsia dentata

In vitro regeneration of Semecarpus anacardium L. from axenic seedling-derived nodal explants

Improved clonal propagation of Spilanthes acmella Murr. for production of scopoletin

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