In vitro plant regeneration via somatic embryogenesis through cell suspension cultures of horsegram [Macrotyloma uniflorum (Lam.) verdc.]

Mohamed, S. Varisai; Wang, C. S.; Thiruvengadam, Muthu; Jayabalan, N.

doi:10.1079/ivp2003524

Cited by 35 publications

(6 citation statements)

References 26 publications

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“…Moreover, somaclonal variations can influence the phenotype of regenerated plants during indirect organogenesis. The explant is considered to be a critical factor that determines the success-or not-of most tissue culture experiments, particularly in somatic embryogenesis (Kantharajah and Golegaonkar 2004;Varisai Mohamed et al 2004). The results of our study demonstrate that it is possible to induce plants from SEs in moth bean.…”

Section: Resultsmentioning

confidence: 59%

Somatic embryogenesis and in vitro plant regeneration in moth bean [Vigna aconitifolia (Jacq.) Marechal]: a recalcitrant grain legume

Choudhary

Singh

Rathore

et al. 2009

Plant Biotechnol Rep

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An efficient in vitro regeneration protocol for moth bean [Vigna aconitifolia (Jacq.) Marechal] via somatic embryogenesis has been developed. Embryogenic callus cultures were established from the cotyledonary node as explant on semi-solid Murashige and Skoog (MS) medium supplemented with 0.75 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.5 mg l -1 6-benzylaminopurine (BA) and with various additives (50 mg l -1 ascorbic acid and 25 mg l -1 each of adenine sulphate, citric acid and L-arginine). Numerous somatic embryos differentiated on MS basal nutrient medium supplemented with 0.25 mg l -1 2,4-D and 0.5 mg l -1 of kinetin (Kin). Sustained cell division resulted in the formation of cell aggregates, which progressed to the globular-and heartshaped somatic embryos and then, if they differentiated properly, to the torpedo shape and cotyledonary stages. The transfer of embryos onto fresh MS basal medium containing 0.2 mg l -1 BA and 2.0 mg l -1 gibberellic acid enabled the embryos to achieve complete maturation and germination. More than 80% of somatic embryos were converted into true-to-type fertile plants. In vitro-regenerated plantlets with well-developed roots were successfully hardened in a greenhouse and established in soil.

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Section: Resultsmentioning

confidence: 59%

Somatic embryogenesis and in vitro plant regeneration in moth bean [Vigna aconitifolia (Jacq.) Marechal]: a recalcitrant grain legume

Choudhary

Singh

Rathore

et al. 2009

Plant Biotechnol Rep

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“…ABA was found to be essential for the maturation process because it increased fatty acid content (Feirer et al 1989) or because it induced specific protein synthesis (Hatzopoulos et al 1990) favoring somatic embryo maturation. ABA has been shown to significantly increase the frequency of the induction of torpedo-shaped embryos and their maturation (Varisai Mohamed et al 2004) and to affect the accumulation of storage protein in maturing embryos (Merkle et al 1995).…”

Section: Resultsmentioning

confidence: 99%

“…This method is suitable for producing explants for somatic embryogenesis in grain legumes (Shekhawat and Galston 1983;Kumar et al 1988;Anbazhagan and Ganapathi 1999;Varisai Mohamed et al 2004). In the present study, a successful protocol has been developed for somatic embryogenesis from leaf-derived callus of black gram.…”

Section: Resultsmentioning

confidence: 99%

Somatic embryo productions by liquid shake culture of embryogenic calluses in Vigna mungo (L.) Hepper

Mookkan

Amutha

Ganapathi

2009

In Vitro Cell.Dev.Biol.-Plant

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The regeneration of plants via somatic embryogenesis liquid shake culture of embryogenic calluses was achieved in Vigna mungo (L.) Hepper (blackgram). The production of embryogenic callus was induced by seeding primary leaf explants of V. mungo onto Murashige and Skoog (MS) (Physiol Plant 15:473-497, 1962) medium supplemented (optimally) with 1.5 mg/l 2,4-dichlorophenoxyacetic acid. The embryogenic callus was then transferred to liquid MS medium supplemented (optimally) with 0.25 mg/l 2,4-dichloro-phenoxyacetic acid. Globular, heart-shaped, and torpedo-shaped embryos developed in liquid culture. The optimal carbohydrate source for production of somatic embryos was 3% sucrose (compared to glucose, fructose, and maltose). L-Glutamine (20 mg/l) stimulated the production of all somatic embryo stages significantly. Torpedo-shaped embryos were transferred to MS (Physiol Plant 15:473-497, 1962) liquid medium containing 0.5 mg/l abscisic acid to induce the maturation of cotyledonary-stage embryos. Cotyledonary-stage embryos were transferred to 1/2-MS semi-solid basal medium for embryo conversion. Approximately 1-1.5% of the embryos developed into plants.

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“…Embryogenic suspension cultures have been established in only few crops, including sweetpotato (Liu et al 2001), cowpea (Prem Anand et al 2000), horsegram (Varisai Mohamed et al 2004). …”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and plant regeneration from cell suspension culture of niger (Guizotia abyssinica Cass.)

Naik

Murthy

2009

Acta Physiol Plant

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Somatic embryogenesis was achieved from cell suspension cultures of niger (Guizotia abyssinica Cass.). Initially, friable embryogenic calluses were induced from cotyledonary leaves of niger on Murashige and Skoog (MS) agar medium containing 5 lM 2,4-Dichlorophenoxyacetic acid (2,4-D) and 0.5 lM kinetin (KIN). Cell suspension cultures were established by using embryogenic calluses in MS liquid medium containing 5 lM 2,4-D and 0.5 lM KIN. Initiation of somatic embryogenesis and development up to globular stage from embryogenic cell clumps occurred in the liquid medium itself. Thereafter embryogenic cell aggregates were transferred to MS agar medium supplemented with 3 lM KIN for embryo differentiation, whereas maturation of somatic embryos occurred in MS agar medium containing 10 lM abscisic acid.

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In vitro plant regeneration via somatic embryogenesis through cell suspension cultures of horsegram [Macrotyloma uniflorum (Lam.) verdc.]

Cited by 35 publications

References 26 publications

Somatic embryogenesis and in vitro plant regeneration in moth bean [Vigna aconitifolia (Jacq.) Marechal]: a recalcitrant grain legume

Somatic embryogenesis and in vitro plant regeneration in moth bean [Vigna aconitifolia (Jacq.) Marechal]: a recalcitrant grain legume

Somatic embryo productions by liquid shake culture of embryogenic calluses in Vigna mungo (L.) Hepper

Somatic embryogenesis and plant regeneration from cell suspension culture of niger (Guizotia abyssinica Cass.)

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