In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L.

Dirks, R.; Buggenum, M van

doi:10.1007/bf00272045

Cited by 58 publications

(39 citation statements)

References 3 publications

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“…1). To date the effect of zeatin has been reported by different research groups (Dirks & Buggenum 1989;Kováč 1992;Malik & Saxena 1992;Gaba et al 1999;Nasircilar et al 2011); similar results were also described in these studies. The shoot regeneration of T. turcica on the MS medium containing different concentrations of NAA was also reported by Cenkci et al (2008).…”

Section: Plant Regenerationsupporting

confidence: 79%

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In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Tekdal

Çetiner

2014

Biologia

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This plant tissue study of micropropagation identifies the selective medium saving for rapid propagation in cultivated Thermopsis turcica, an endangered germplasm of the family Fabaceae. The aim is to obtain the optimum growth medium of T. turcica by enabling the in vitro propagation of this endemic. In this study, the leaves and stems of T. turcica were cultured on a Murashige and Skoog's medium supplemented with various concentrations (0.5, 1.0 and 2.0 mg L −1 1-Naphthaleneacetic acid) of auxin and (0.2 and 0.5 mg L −1 Zeatin) (1.0 and 2.0 mg L −1 Benzylaminopurine) of cytokinins. Previous research focused on the regeneration from the seed of T. turcica Eber population; we concentrated upon the regeneration of different plant parts (leaf and stem) of T. turcica Aksehir population. In addition, according to the literature on T. turcica that to date the effects of Zeatin on the regeneration has not been performed. The most promising regeneration and growth were obtained from leaf explants cultured on the media with 2.0 mg L −1 1-Naphthaleneacetic acid and 0.5 mg L −1 Zeatin (93.3%). The regenerated plantles were rooted on the media containing 2.0 mg L −1 Indole-3-butyric acid. Rooted plantlets were transplanted into potting of sterilized soil. The present study reports on the sufficient in vitro regeneration protocol through organogenesis in T. turcica. The findings presented here have implications for in vitro protection and use of this endemic endangered species in further biotechnological research.

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Section: Plant Regenerationsupporting

confidence: 79%

“…The explants were then rinsed three consecutive times with sterile distilled water in a laminar flow cabinet (Dirks & Buggenum 1989;Kováč 1992).…”

Section: Surface Sterilizationmentioning

confidence: 99%

In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Tekdal

Çetiner

2014

Biologia

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“…(KATHAL et al, 1995). Entre os diferentes tipos de explantes utilizados para regeneração em melão, secções foliares e cotiledonares têm apresentado maior taxa de regeneração (MOLINA & NUEZ, 1995), embora os últimos apresentem resposta mais rápida (DIRKS & VAN BUGGENUM, 1989).…”

Section: Introductionunclassified

Regeneração in vitro de melão, cv. 'Gaúcho'

et al. 2010

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“…Although melon is susceptible to Agrobacterium infection and is responsive to regeneration from explants derived from the cotyledon (Dirks and van Buggenum, 1989) and callus (Moreno et al, 1985), the transformation regeneration frequency varies widely among cultivars (Galperin et al, 2003;Akasaka-Kennedy et al, 2004;Rhimi et al, 2006;Hao et al, 2011) and sources of explants (Fang and Grumet, 1990;Guis et al, 2000;Ren et al, 2012). Moreover, in melon transformation, a high frequency of tetraploid and false-positive regenerants are commonly observed.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of the CmACS-3 gene in melon causes abnormal pollen development

Zhang¹,

Luan²

2015

Genet. Mol. Res.

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ABSTRACT. Sexual diversity expressed by the Curcurbitaceae family is a primary example of developmental plasticity in plants. Most melon genotypes are andromonoecious, where an initial phase of male flowers is followed by a mixture of bisexual and male flowers. Overexpression of the CmACS-3 gene in melon plants showed an increased number of flower buds, and increased femaleness as demonstrated by a larger number bisexual buds. Transformation of CmACS-3 in melons showed earlier development of and an increased number of bisexual buds that matured to anthesis but also increased the rate of development of the bisexual buds to maturity. Field studies showed that CmACS-3-overexpressing melons had earlier mature bisexual flowers, earlier fruit set, and an increased number of fruits set on closely spaced nodes on the main stem.

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In vitro plant regeneration from leaf and cotyledon explants of Cucumis melo L.

Cited by 58 publications

References 3 publications

In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Regeneração in vitro de melão, cv. 'Gaúcho'

Overexpression of the CmACS-3 gene in melon causes abnormal pollen development

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