In vitro Plant Regeneration from Shoot tip Explants of Exacum travancoricum Beedi

Janarthanam, B.; Sumathi, E.

doi:10.3329/ptcb.v20i2.6889

Cited by 9 publications

(5 citation statements)

References 1 publication

(1 reference statement)

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“…1A,B,C,D,E). This synergistic combination of auxin and cytokinin on organogenic differentiation has been well explained in plant tissue culture (Baskaran and Jayabalan 2005;Gururaj et al, 2007;Janarthanam and Seshadri 2008;Ahmad et al, 2010;Janarthanam and Sumathi, 2010). The combination of BAP at 1.0 mg/l along with addition of IAA (0.01 -0.1 mg/l) was effective for stimulating regeneration of shootlets.…”

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confidence: 72%

Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

Janarthanam

Ethiraj

2013

Bangladesh J. Sci. Ind. Res.

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A protocol for multiple shoot induction and plant regeneration from nodal explants of Ocimum citriodorum has been developed. Nodal explants inoculated on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l Benzyl adenine (BAP) and 0.025 mg/l indole -3- acetic acid (IAA) showed better growth response (80%) and produced 15.2 ± 1.28 shoots per explant with an average length of 6.17 ± 0.29 cm after 35 days. Roots were induced after transfer to half strength MS medium supplemented with 0.5 mg/l Indole -3- butyric acid (IBA) produced 6.0 ± 1.0 roots with an average height of 4.9 ± 0.26 cm after 30 days. Plantlets with well developed root and shoot systems were successfully acclimated (80 %) and established in earthen pots containing mixture of soil, vermiculite and farm yard manure (1:1:1). Bangladesh J. Sci. Ind. Res. 47(4), 433-436, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.8547

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confidence: 72%

Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

Janarthanam

Ethiraj

2013

Bangladesh J. Sci. Ind. Res.

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“…At lower and higher concentrations of BAP (1.11 and 8.88 µM), TDZ (1.14 and 9.08 µM) and Kn (1.16 and 9.20 μM) did not significantly induce the number of shoots per explant. BAP has been considered to be one of the most active cytokinins for organogenic differentiation in plant tissue culture (Janarthanam et al 2009and 2011, Biswas et al 2009, Sudharson et al 2014. Nodal explants on MS without growth regulator did not initiate shoot differentiation.…”

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confidence: 99%

In vitro Plant Regeneration from Nodal Explants of Coleus forskohlii Briq. - An Important Medicinal Plant

Janarthanam¹,

Sumathi

2020

Plant Tissue Cult. & Biotech.

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An efficient in vitro mass propagation and promising protocol has been successfully standardized and developed for Coleus forskohlii through direct organogenesis from nodal explants. Nodal explants cultured onto MS basal medium supplemented with 4.44 μM BAP recorded the highest response and produced 24.3 ± 0.2 shoots per explant with an average shoot length 5.6 ± 0.4 cm after 30 days of culture. The in vitro shoots recorded higher response for development of rooting on half strength MS fortified with 2.46 μM IBA which produced the best response 7.8 ± 0.6 roots per in vitro shoot with an average root length of 4.3 ± 0.1 cm after 25 days. The in vitro rooted plantlets were transferred for hardening and 90% of the plantlets survived were successfully acclimatized and established in small plastic pots. This protocol recorded to be a highly repeatable, successful and rapid technique that could be utilized for the commercial mass propagation and ex situ conservation of Coleus forskohlii. It is important to note that the morphology of the in vitro plantlets of Coleus forskohlii showed a true-to-type growth habit, both in vitro and when transferred to ex vitro growth conditions. Plant Tissue Cult. & Biotech. 30(1): 143-148, 2020 (June)

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“…BAP has been considered to be one of the most active cytokinins in organogenic differentiation in plant tissue culture (Gururaj et al 2007, Fracaro and Echeverrigaray 2004, Janarthanam et al 2009). Coconut milk (CM) is an undefined complex mixture of organic substances that has been successfully employed for culturing different plant species (Arditti and Ernst 1993, Suttle 1996, Janarthanam et al 2011.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

Janarthanam

Rashmi²,

Sumathi³

2012

Plant Tissue Cult. & Biotech.

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An efficient plant regeneration protocol was developed for Artemisia annua, an aromatic medicinal herb. Nodal explants inoculated on MS supplemented with 4.44 μM BAP showed better growth response and produced 116.2 ± 0.1 microshoots of an average length 1.9 ± 0.3 cm after 35 days culture. The cluster of small shootlets were cultured on shoot elongation medium supplemented with 1.44 μM GA3 and 10% coconut milk (CM) showed shoot elongation up to 4.6 ± 0.7 cm. Roots were induced after transfer to half strength MS supplemented with 2.46 μM IBA produced 14.3 ± 0.2 roots with an average height of 4.3 ± 0.53 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 : 1 : 1).

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In vitro Plant Regeneration from Shoot tip Explants of Exacum travancoricum Beedi

Cited by 9 publications

References 1 publication

Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

In vitro Plant Regeneration from Nodal Explants of Coleus forskohlii Briq. - An Important Medicinal Plant

Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

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