In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine

Tapajós, E.C.C.; Longo, João Paulo Figueiró; Simioni, Andreza Ribeiro; Lacava, Z.G.M.; Santos, Marcos Ferracioli; Morais, P.C.; Tedesco, Antônio Cláudio; Azevedo, Ricardo Bentes

doi:10.1016/j.oraloncology.2008.01.013

Cited by 49 publications

(38 citation statements)

References 22 publications

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“…These observations are in accordance with other reports, which identified a significantly increased intracellular oxidative stress when high-light energies were applied in the PDT protocols in comparison with low-light energy protocols. 44,45 When oxidative stress is too intense, as observed in the high-energy groups, the cellular machinery that initiates apoptosis is also affected, and cell death occurs via necrosis. 45,46 Thus, at lower energy doses, limited damage is caused to cell structures.…”

Section: Photodynamic Activity Against Cells In Vitromentioning

confidence: 99%

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Muehlmann¹,

Chiyin²,

Longo³

et al. 2014

IJN

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Photodynamic therapy is generally considered to be safer than conventional anticancer therapies, and it is effective against different kinds of cancer. However, its clinical application has been significantly limited by the hydrophobicity of photosensitizers. In this work, a system composed of the hydrophobic photosensitizer aluminum–phthalocyanine chloride (AlPc) associated with water dispersible poly(methyl vinyl ether-co-maleic anhydride) nanoparticles is described. AlPc was associated with nanoparticles produced by a method of solvent displacement. This system was analyzed for its physicochemical characteristics, and for its photodynamic activity in vitro in cancerous (murine mammary carcinoma cell lineage 4T1, and human mammary adenocarcinoma cells MCF-7) and noncancerous (murine fibroblast cell lineage NIH/3T3, and human mammary epithelial cell lineage MCF-10A) cell lines. Cell viability and the elicited mechanisms of cell death were evaluated after the application of photodynamic therapy. This system showed improved photophysical and photochemical properties in aqueous media in comparison to the free photosensitizer, and it was effective against cancerous cells in vitro.

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Section: Photodynamic Activity Against Cells In Vitromentioning

confidence: 99%

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Muehlmann¹,

Chiyin²,

Longo³

et al. 2014

IJN

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show abstract

“…Their formation results from constitutional and/or inherited factors combined with environmental factors. Skin carcinoma are usually treated by surgical excision [2], radiotherapy [3][4][5], irradiation [6] or dynamic phototherapy [7]. However, these treatments are painful and invasive.…”

Section: Introductionmentioning

confidence: 99%

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Overstraeten-Schlögel

Ho-Shim

Tevel

et al. 2012

Journal of Biomaterials Science, Polymer Edition

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Skin carcinoma are among the most spread diagnosed tumours in the world. In this study, we investigated the transfection of immortalized keratinocytes, used as an in vitro model for skin carcinoma, using antisense technology and poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA)-based polymers, with original architecture and functionalities. We tested PDMAEMA polymers with different structures: linear, with two (DEA-PDMAEMA) or three (TEA-PDMAEMA) arms. The cytotoxicity of these polymers was assessed over a wide range of apparent M n (from 7600 to 64 600). At a N/P ratio of 7.38, cytotoxicity increases with the M n . Keratinocytes were transfected with a fluorescent oligonucleotide and then analyzed by flow cytometry. For the three architectures tested, the percentage of transfected cells and abundance of internalized oligonucleotide were closely related to the M n of the polymer. Confocal microscopy and FACS analyses showed a wide spread fine granular distribution of the oligonucleotide up to 3 days post-transfection. Then, we assessed the silencing efficiency of the polymers, targeting GFP in GFP expressing keratinocytes. The maximal silencing effect (±40%) was obtained using a DEA-PDMAEMA polymer (M n = 30 300). These results suggest that PDMAEMA-based polymers can be efficiently used to transfect immortalized keratinocytes and, thus, open new perspectives in the therapy of skin carcinoma.

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“…SCC are the second most frequent type with an occurrence of more than 750 000 cases diagnosed worldwide each year. The present treatments include surgical excision (2), radiotherapy (3)(4)(5), irradiation (6) or dynamic phototherapy (7). However these treatments are painful and mutilating.…”

Section: Original Articlementioning

confidence: 99%

Assessment of new biocompatible Poly(N-(morpholino)ethyl methacrylate)-based copolymers by transfection of immortalized keratinocytes

Overstraeten-Schlögel

Shim

Tevel

et al. 2012

Drug Delivery

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In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine

Cited by 49 publications

References 22 publications

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Assessment of new biocompatible Poly(N-(morpholino)ethyl methacrylate)-based copolymers by transfection of immortalized keratinocytes

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In vitro photodynamic therapy on human oral keratinocytes using chloroaluminum-phthalocyanine

Cited by 49 publications

References 22 publications

Aluminum&ndash;phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Aluminum&ndash;phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Transfection of Immortalized Keratinocytes by Low Toxic Poly(2-(Dimethylamino)Ethyl Methacrylate)-Based Polymers

Assessment of new biocompatible Poly(N-(morpholino)ethyl methacrylate)-based copolymers by transfection of immortalized keratinocytes

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Product

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Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy

Aluminum–phthalocyanine chloride associated to poly(methyl vinyl ether-co-maleic anhydride) nanoparticles as a new third-generation photosensitizer for anticancer photodynamic therapy