In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains

Mei, Yingjuan; Xiao, Xiangling; Fan, Lili; Liu, Qichao; Zheng, Mengxue; Hamblin, Michael R.; Ni, Bing; Yin, Rui

doi:10.1016/j.pdpdt.2019.06.003

Cited by 18 publications

(9 citation statements)

References 37 publications

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“…After cytotoxic singlet oxygen is produced, the procedure causes vascular endothelial cell death through apoptosis, necrosis or autophagy, resulting in thrombosis and vascular occlusion. 27,28 PDT can theoretically target ectatic capillaries of all diameters and, in contrast to PDL, can induce vascular damage deeper in the dermis, with a considerably reduced risk of epidermal necrosis due to its vascular-selective characteristics. 29 Previous studies have also demonstrated that PDT is an effective and safe means of improving the appearance of PWS.…”

Section: Introductionmentioning

confidence: 99%

“…The principal components of PDT are oxygen, a photosensitizing agent and excitation light. After cytotoxic singlet oxygen is produced, the procedure causes vascular endothelial cell death through apoptosis, necrosis or autophagy, resulting in thrombosis and vascular occlusion . PDT can theoretically target ectatic capillaries of all diameters and, in contrast to PDL, can induce vascular damage deeper in the dermis, with a considerably reduced risk of epidermal necrosis due to its vascular‐selective characteristics .…”

Section: Introductionmentioning

confidence: 99%

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Retrospective study of photodynamic therapy for pulsed dye laser‐resistant port‐wine stains

Han

Ying

Zhang

et al. 2020

The Journal of Dermatology

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Pulsed dye laser‐resistant port‐wine stains present a therapeutic challenge. The aim of this study was to evaluate the efficacy and safety of photodynamic therapy for treating these lesions. A total of 67 patients with pulsed dye laser‐resistant cervicofacial port‐wine stains were retrospectively assessed after undergoing photodynamic therapy mediated with a combination of hemoporfin and 532‐nm light. For objective evaluation of photodynamic therapy efficacy, first, the colorimetric changes in the port‐wine stain lesions were evaluated according to the L*a*b* color coordinate system, then the values of color changes (ΔE) and blanching rate were calculated. For subjective evaluation of improvement, photographs taken before and after photodynamic therapy were evaluated by three independent assessors blindly. Patient satisfaction was also used as a factor in the subjective evaluation. Adverse events were recorded after treatment. The median ΔE decreased significantly from the pretreatment value of 13.42 to 9.90 at the 2‐month follow up (P < 0.001). The median blanching rate of port‐wine stains was 28.04% after an average of 1.21 sessions of photodynamic therapy. Based on the overall visual assessment, 46.2% patients showed excellent or good levels of improvement (>50% color blanching). Adverse events were minimal, transient and self‐limiting. In conclusion, photodynamic therapy serves as an alternative means to treat pulsed dye laser‐resistant port‐wine stains.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Retrospective study of photodynamic therapy for pulsed dye laser‐resistant port‐wine stains

Han

Ying

Zhang

et al. 2020

The Journal of Dermatology

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“…This porphyrin has the characteristics of high photoactivity and low toxicity. HMME at a certain concentration exhibits four di®erent absorption peaks within the range of 500-800 nm, 14,15 which enables the selection of excitation wavelength according to the existing clinical conditions in the practical application process and has a high clinical applicability.…”

Section: Introductionmentioning

confidence: 99%

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Liang

Zou

et al. 2020

J. Innov. Opt. Health Sci.

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The main objective of this study is to evaluate the antibacterial effect of antibacterial photodynamic therapy (aPDT) on Streptococcus mutans (S. mutans) biofilm model in vitro. The selection of photosensitizers is the key step for the efficacy of photodynamic therapy (PDT). However, no studies have been conducted in the oral field to compare the functional characteristics and application effects of PDT mediated by various photosensitizers. In this research, the antibacterial effect of Methylene blue (MB)/650[Formula: see text]nm laser and Hematoporphyrin monomethyl ether (HMME)/532[Formula: see text]nm laser on S. mutans biofilm was compared under different energy densities to provide experimental reference for the clinical application of the two PDT. The yield of lactic acid was analyzed by Colony forming unit (CFU) and spectrophotometry, and the complete biofilm activity was measured by Confocal Laser Scanning Microscopy (CLSM) to evaluate the bactericidal effect on each group. Based on the results of CFU, the bacterial colonies formed by 30.4[Formula: see text]J/cm2 532[Formula: see text]nm MB-aPDT group and 30.4[Formula: see text]J/cm2 532[Formula: see text]nm HMME-aPDT group were significantly less than those in other groups, and the bacterial colonies in HMME-aPDT group were less than those in HMME-aPDT group. Lactic acid production in all treatment groups except the photosensitizer group was statistically lower than that in the normal saline control group. The activity of bacterial plaque biofilm was significantly decreased in the two groups treated with 30.4[Formula: see text]J/cm2 aPDT. Therefore, aPDT suitable for energy measurement can kill S. mutans plaque biofilm, and MB-aPDT is better than HMME-aPDT.

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“…In comparison with the commonly used PpIX, HMME exhibits similar chemical structure, absorption peaks (400, 503, 539, 565, and 617 nm), and singlet oxygen quantum yield (0.6; Lei et al, 2012 ). Although HMME has been approved in China for the photodynamic treatment of naevus flammeus, showing high effectiveness and safety ( Mei et al, 2019 ), it is still not expanded in the photodynamic treatment of bacteria or fungi induced skin diseases. AE is a newly discovered natural anthraquinone PS isolated from Chinese traditional herbs, possessing absorption in the blue region (430 nm) and singlet oxygen quantum yield of 0.57, which may be suitable for the treatment of superficial lesions ( Zang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Antifungal Effect of Antimicrobial Photodynamic Therapy Mediated by Haematoporphyrin Monomethyl Ether and Aloe Emodin on Malassezia furfur

et al. 2021

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Infectious dermatological diseases caused by Malassezia furfur are often chronic, recurrent, and recalcitrant. Current therapeutic options are usually tedious, repetitive, and associated with adverse effects. Alternatives that broaden the treatment options and reduce side effects for patients are needed. Antimicrobial photodynamic therapy (aPDT) is an emerging approach that is quite suitable for superficial infections. The aim of this study is to investigate the antimicrobial efficacy and effect of aPDT mediated by haematoporphyrin monomethyl ether (HMME) and aloe emodin (AE) on clinical isolates of M. furfur in vitro. The photodynamic antimicrobial efficacy of HMME and AE against M. furfur was assessed by colony forming unit (CFU) assay. The uptake of HMME and AE by M. furfur cells was investigated by fluorescence microscopy. Reactive oxygen species (ROS) probe and flow cytometry were employed to evaluate the intracellular ROS level. The effect of HMME and AE-mediated aPDT on secreted protease and lipase activity of M. furfur was also investigated. The results showed that HMME and AE in the presence of light effectively inactivated M. furfur cells in a photosensitizer (PS) concentration and light energy dose-dependent manner. AE exhibited higher antimicrobial efficacy against M. furfur than HMME under the same irradiation condition. HMME and AE-mediated aPDT disturbed the fungal cell envelop, significantly increased the intracellular ROS level, and effectively inhibited the activity of secreted protease and lipase of M. furfur cells. The results suggest that HMME and AE have potential to serve as PSs in the photodynamic treatment of dermatological diseases caused by M. furfur, but further ex vivo or in vivo experiments are needed to verify that they can meet the requirements for clinical practice.

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In vitro photodynamic therapy of endothelial cells using hematoporphyrin monomethyl ether (Hemoporfin): Relevance to treatment of port wine stains

Cited by 18 publications

References 37 publications

Retrospective study of photodynamic therapy for pulsed dye laser‐resistant port‐wine stains

Retrospective study of photodynamic therapy for pulsed dye laser‐resistant port‐wine stains

Effect of antibacterial photodynamic therapy on Streptococcus mutans plaque biofilm in vitro

Antifungal Effect of Antimicrobial Photodynamic Therapy Mediated by Haematoporphyrin Monomethyl Ether and Aloe Emodin on Malassezia furfur

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