In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells

Soares, Betânia Maria; Silva, Danielle Letícia da; Sousa, Gerdal Roberto de; Amorim, José Cláudio Faria; Resende, Maria Aparecida de; Pinotti, Marcos; Cisalpino, Patrícia Silva

doi:10.1016/j.jphotobiol.2008.07.013

Cited by 72 publications

(83 citation statements)

References 27 publications

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“…Besides the known fungicidal activity of APDI (7,11,24), we observed that sublethal photodynamic action had a temporarily fungistatic effect on C. albicans, and the extent of this activity was dependent on the amount of energy delivered to the cell-PS system, even under conditions bellow the photoinactivation threshold. The increase in lag phase observed immediately after APDI suggested that cell growth was arrested.…”

Section: Discussionmentioning

confidence: 79%

“…Recently, studies have focused the investigation of APDI effects on key elements of the microbial phenotype. These aspects of APDI have been only sporadically explored; there are scattered reports that the production of ROS from APDI can change the virulence profiles of bacteria (22,23) and fungi (6,7). However, the consequences of APDI to further generations of the treated pathogens remain under investigation.…”

Section: Discussionmentioning

confidence: 99%

“…The production of ROS in APDI has been implicated in two important aspects of microbial physiology: (i) changes in the expression of virulence determinants of yeasts (6,7) and (ii) the impact of APDI on the overall survival of microorganisms. Moreover, some types of PS are able to penetrate the microbial cell and bind to cytoplasmic components and nuclear material.…”

mentioning

confidence: 99%

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Antimicrobial Photodynamic Inactivation Inhibits Candida albicans Virulence Factors and Reduces In Vivo Pathogenicity

Kato

Prates

Sabino

et al. 2013

Antimicrob Agents Chemother

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The objective of this study was to evaluate whether Candida albicans exhibits altered pathogenicity characteristics following sublethal antimicrobial photodynamic inactivation (APDI) and if such alterations are maintained in the daughter cells. C. albicans was exposed to sublethal APDI by using methylene blue (MB) as a photosensitizer (0.05 mM) combined with a GaAlAs diode laser ( 660 nm, 75 mW/cm 2 , 9 to 27 J/cm 2 ). In vitro, we evaluated APDI effects on C. albicans growth, germ tube formation, sensitivity to oxidative and osmotic stress, cell wall integrity, and fluconazole susceptibility. In vivo, we evaluated C. albicans pathogenicity with a mouse model of systemic infection. Animal survival was evaluated daily. Sublethal MB-mediated APDI reduced the growth rate and the ability of C. albicans to form germ tubes compared to untreated cells (P < 0.05). Survival of mice systemically infected with C. albicans pretreated with APDI was significantly increased compared to mice infected with untreated yeast (P < 0.05). APDI increased C. albicans sensitivity to sodium dodecyl sulfate, caffeine, and hydrogen peroxide. The MIC for fluconazole for C. albicans was also reduced following sublethal MB-mediated APDI. However, none of those pathogenic parameters was altered in daughter cells of C. albicans submitted to APDI. These data suggest that APDI may inhibit virulence factors and reduce in vivo pathogenicity of C. albicans. The absence of alterations in daughter cells indicates that APDI effects are transitory. The MIC reduction for fluconazole following APDI suggests that this antifungal could be combined with APDI to treat C. albicans infections. Management of infections caused by clinically relevant fungal pathogens is a challenge due to the incidence of resistance that can develop during therapy, especially in immunocompromised individuals (1). The increasing need for prolonged use of antifungal drugs, longer than usually recommended for antibiotics, is accompanied by a corresponding increased incidence of side effects. Furthermore, the limited number of available antifungal compounds and the need to determine the susceptibility profile of the organism also complicate the treatment of these infections (2).The fungal species Candida albicans is part of the commensal flora of the human gastrointestinal and genitourinary tracts and can cause superficial infections of the mucosa and skin (3, 4). The infection depends on imbalances between increased C. albicans virulence attributes and impaired host defense systems. In immunocompromised individuals, however, C. albicans may invade deeper tissues, penetrate the blood vessels, and cause life-threatening systemic infections (3, 5).Photodynamic therapy (PDT) is a light-based treatment platform that is under development for several applications in oncology, dermatology, and ophthalmology, and it has recently been investigated as an antimicrobial therapy. The combination of nontoxic dyes referred to as photosensitizers (PS) and harmless low-intensity visible light generate...

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Section: Discussionmentioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Antimicrobial Photodynamic Inactivation Inhibits Candida albicans Virulence Factors and Reduces In Vivo Pathogenicity

Kato

Prates

Sabino

et al. 2013

Antimicrob Agents Chemother

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“…Recently, alternative light sources, such as LED, have been successfully used in PDT. 25,26,44 In the present investigation, LED was used as a light source because of its ability to irradiate larger areas than is possible with collimated laser light. Moreover, LED technology is simpler and has a lower cost than laser.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

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130

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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“…There is a wealth of literature that focuses on PDTbased antibiofilm strategies against a variety of microbial species [26][27][28]. On the contrary, the effects of PDT on phenotypic biofilm elements (e.g., adhesions and extracellular polysaccharide) are poorly investigated [29,30]. Staphylococci are one of the most important human pathogens and a major cause of morbidity and mortality worldwide.…”

Section: Pdt For Inactivate Biofilm Formationmentioning

confidence: 99%

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

Bloise¹,

Minzioni²,

Imbriani³

et al. 2017

Photomedicine - Advances in Clinical Practice

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Recent developments in light-controlled therapies (e.g., photodynamic and photothermal therapies) provide promising strategies to prevent and suppress bacterial infections, which are a leading cause of morbidity and mortality. Antibacterial photodynamic therapy (aPDT) has drawn increasing attention from the scientific society for its potential to kill multidrug-resistant pathogenic bacteria and for its low tendency to induce drug resistance. In this chapter, we summarize the mechanism of action of aPDT, the photosensitizers, as well the current developments in terms of treating Gram-positive and Gram-negative bacteria. The chapter also describes the recent progress relating to photomedicine for preventing bacterial infections and biofilm formation. We focus on the laser device used in aPDT and on the light-treatment parameters that may have a strong impact on the results of aPDT experiments. In the last part of this chapter, we survey on the various nanoparticles delivering photoactive molecules, and photoactive-nanoparticles that can potentially enhance the antimicrobial action of aPDT.

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In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells

Cited by 72 publications

References 27 publications

Antimicrobial Photodynamic Inactivation Inhibits Candida albicans Virulence Factors and Reduces In Vivo Pathogenicity

Antimicrobial Photodynamic Inactivation Inhibits Candida albicans Virulence Factors and Reduces In Vivo Pathogenicity

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?

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