In vitro permeation of gold nanoparticles through rat skin and rat intestine: Effect of particle size

Sonavane, Ganeshchandra S.; Tomoda, Keishiro; Sano, Akira; Ohshima, Hiroyuki; Terada, Hiroshi; Makino, Kimiko

doi:10.1016/j.colsurfb.2008.02.013

Cited by 317 publications

(215 citation statements)

References 39 publications

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“…We observed that the ζ potential of free GNRs is +20.71 mV, and upon successful complex formation to 5′PPP-ssRNA, CIAP-ssRNA, and Capped-ssRNA, it decreased to −9.91, −9.61, and −8.23 mV, respectively (Table S1). These results suggest that binding of cationic GNRs to anionic nucleic acid material leads to a slightly negatively charged nanoplex and that complexing of genetic material to GNRs would increase uptake of the nanoplexes into the target cell due to evasion of the reticuloendothelial system and reduction in nonspecific interactions with proteins and other biomolecules as demonstrated by other studies (30,35).…”

Section: Resultssupporting

confidence: 68%

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Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication

Chakravarthy

Bonoiu

Davis

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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The emergence of the pandemic 2009 H1N1 influenza virus has become a world-wide health concern. As drug resistance appears, a new generation of therapeutic strategies will be required. Here, we introduce a nanotechnology approach for the therapy of pan-demic and seasonal influenza virus infections. This approach uses gold nanorods (GNRs) to deliver an innate immune activator, pro-ducing a localized therapeutic response. We demonstrated the utility of a biocompatible gold nanorod, GNR-5′PPP-ssRNA nanoplex, as an antiviral strategy against type A influenza virus. In human respiratory bronchial epithelial cells, this nanoplex activated the retinoic acid-inducible gene I (RIG-I) pathogen recognition pathway, resulting in increased expression of IFN-β and other IFN-stimulated genes (ISGs) (e.g., PKR, MDA5, IRF1, IRF7, and MX1 ). This increase in type I IFN and ISGs resulted in a decrease in the replication of H1N1 influenza viruses. These findings suggest that further evaluation of biocompatible nanoplexes as unique antivirals for treatment of seasonal and pandemic influenza viruses is warranted.

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Section: Resultssupporting

confidence: 68%

“…In recent years, gold nanoparticles (GNPs) and nanorods (GNRs) have gained increasing interest as potential biocompatible and site-specific carriers of various diagnostic and therapeutic agents (27)(28)(29)(30)(31). Recently, we used GNR to deliver siRNA to silence genes that are associated with opiate drug addiction (32,33).…”

mentioning

confidence: 99%

Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication

Chakravarthy

Bonoiu

Davis

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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“…Few literature reports deal with gold nanoparticles effects on cultures of skin-derived cell lines [57] and gold nanoparticle penetration (less than 15 nm) in thawed human skin [58,59]. In our research, colloidal stable gold nanoparticles have been prepared with 3MPS thiol as functionalizing layer, following the hereafter reported synthetic scheme (see Fig.…”

Section: Resultsmentioning

confidence: 99%

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Bessar

Venditti

Benassi

et al. 2016

Colloids and Surfaces B: Biointerfaces

111

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a b s t r a c tGold nanoparticles (AuNPs) represent an effective choice for topical drug delivery systems thanks to their small size, general non-toxicity, ease of functionalization and high surface to volume ratio. Even if systemic, methotrexate still plays an important role in psoriasis treatment: its topical use shows insufficient percutaneus penetration owing to limited passive diffusion, high molecular weight and dissociation at physiological pH. The aim of our study was to design a new drug delivery nanocarrier for Methotrexate and to improve its solubility, stability and biodistribution. AuNPs were on purpose prepared with a hydrophilic stabilizing layer, in order to improve the colloidal stability in water. Watersoluble gold nanoparticles functionalized by sodium 3-mercapto-1-propansulfonate (Au-3MPS) were prepared and loaded with methotrexate (MTX). The loading efficiency of MTX on Au-3MPS was assessed in the range 70-80%, with a fast release (80% in one hour). The release was studied up to 24 h reaching the value of 95%. The Au-3MPS@MTX conjugate was fully characterized by spectroscopic techniques (UV-vis, FTIR) and DLS. Preliminary toxicity tests in the presence of keratinocytes monolayers allowed to assess that the used Au-3MPS are not toxic. The conjugate was then topically used on C57BL/6 mouse normal skin in order to trace the absorption behavior. STEM images clearly revealed the distribution of gold nanoparticles inside the cells. In vitro studies showed that Methotrexate conjugated with Au-3MPS is much more efficient than Methotrexate alone. Moreover, DL50, based on MTT analysis, is 20 folds reduced at 48 h, by the presence of nanoparticles conjugation. UV-vis spectra for in vivo tracing of the conjugate on bare mouse skin after 24 h of application, show increased delivery of Methotrexate in the epidermis and dermis using Au-3MPS@MTX conjugate, compared to MTX alone. Moreover we observed absence of the Au-3MPS in the dermis and in the epidermis, suggesting that these layers of the skin do not retain the nanoparticles. Based on our data, we found that the novel Au-3MPS@MTX conjugate is an effective non-toxic carrier for the satisfactory percutaneous absorption of Methotrexate and could help in possible topical treatment of psoriasis.

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“…Sonanave et al 29 evaluated the role of GNP particle size (15, 102, and 198 nm) in penetration in rat skin. GNPs showed negative zeta potential and, the smaller the particle size, the higher the penetration rates in tissues.…”

Section: Discussionmentioning

confidence: 99%

Gold nanoparticles and diclofenac diethylammonium administered by iontophoresis reduce inflammatory cytokines expression in Achilles tendinitis

Venancio¹,

Possato²,

Zeferino³

et al. 2012

IJN

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Introduction Tendinitis affects a substantial number of people in several occupations involving repetitive work or direct trauma. Iontophoresis is a therapeutic alternative used in the treatment of injury during the inflammatory phase. In recent years, gold nanoparticles (GNP) have been studied due to their therapeutic anti-inflammatory capacity and as an alternative to the transport of several proteins. Purpose This study evaluates the therapeutic effects of iontophoresis using GNPs and diclofenac diethylammonium on inflammatory parameters in rats challenged with traumatic tendinitis. Methods Wistar rats were divided in three treatment groups (n = 15): (1) iontophoresis + diclofenac diethylammonium; (2) iontophoresis + GNP; and (3) iontophoresis + diclofenac diethylammonium + GNP. External control was formed by challenged tendons without treatment (n = 15). Iontophoresis was administered using 0.3 mA direct current on 1.5 cm 2 electrodes. Results The levels of both inflammatory cytokines were significantly higher in untreated challenged rats, when compared with the control (5.398 ± 234 for interleukin 1 beta and 6.411 ± 432 for tumor necrosis factor alpha), which confirms the occurrence of an inflammatory stage in injury ( P < 0.05). A significant decrease was observed in expression of cytokines interleukin 1 beta in the three treatment groups, in comparison with untreated challenged tendons, although, in the group treated with diclofenac and GNP, results were similar to the control (1.732 ± 239) ( P < 0.05). Concerning tumor necrosis factor alpha, only the group treated with the association diclofenac and GNPs presented decreased levels, compared with the control (3.221 ± 369) ( P < 0.05). Conclusion The results show the efficacy of drug administration using direct current to treat tendinitis in an animal model, and the potential anti-inflammatory, carrier, and enhancing effects of GNPs in iontophoresis.

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In vitro permeation of gold nanoparticles through rat skin and rat intestine: Effect of particle size

Cited by 317 publications

References 39 publications

Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication

Gold nanorod delivery of an ssRNA immune activator inhibits pandemic H1N1 influenza viral replication

Functionalized gold nanoparticles for topical delivery of methotrexate for the possible treatment of psoriasis

Gold nanoparticles and diclofenac diethylammonium administered by iontophoresis reduce inflammatory cytokines expression in Achilles tendinitis

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