In vitro percutaneous penetration of silver nanoparticles in pig and human skin

Kraeling, Margaret E. K.; Topping, Vanessa; Keltner, Zachary; Belgrave, Kathleen; Bailey, Keenan D.; Gao, Xiugong; Yourick, Jeffrey J.

doi:10.1016/j.yrtph.2018.04.006

Cited by 55 publications

(39 citation statements)

References 38 publications

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“…The oil-in-water (O/W) emulsion vehicle used in the skin absorption studies comprised the following ingredients and concentrations: polyglyceryl-3 distearate (BASF Corp., Parsippany, NJ) 3%, mineral oil light (Penreco, Karns City, PA) 10%, cetearyl alcohol (Henkel Corp., Hoboken, NJ) 3%, propylene glycol (Aldrich Chemical Co., Milwaukee, WI) 5%, potassium phosphate-sodium hydroxide buffer (pH 7) 78%, methyl paraben (Pfaltz & Bauer, Inc., Stamford, CT) 0.5%, and propyl paraben (Pfaltz & Bauer, Inc.) 0.5%. 12,13 Composition of the receptor fluid HEPES-buffered Hank's balanced salt solution…”

Section: Maldi-tof Mass Spectrometrymentioning

confidence: 99%

“…The in vitro percutaneous absorption studies were conducted in human and pig skin. 13,15 Human cadaver abdominal skin was obtained from the National Disease Research Interchange (NDRI, Philadelphia, PA). Full-thickness frozen skin was from Caucasian female donors (three subjects/condition) ranging in age from 28 to 75 years and was stored at À80°C and thawed to room temperature before use.…”

Section: Percutaneous Absorption Experimentsmentioning

confidence: 99%

“…18 A Padgett dermatome (Integra Life Sciences, Plainsboro, NJ) was used to cut the skin to a thickness of *240-450 lm for human skin and 300-550 lm for pig skin. 13,19 A 17-mm-diameter biopsy punch was used to prepare skin disks, which were then placed epidermal-side up in Teflon flow-through in-line diffusion cells (PermeGear, Hellertown, PA). 13,20 The skin disks and diffusion cells were perfused with filtered HHBSS receptor fluid (pH = 7.4) at a flow rate of *1.5 mL/h for the duration of the study.…”

Section: Percutaneous Absorption Experimentsmentioning

confidence: 99%

“…13,19 A 17-mm-diameter biopsy punch was used to prepare skin disks, which were then placed epidermal-side up in Teflon flow-through in-line diffusion cells (PermeGear, Hellertown, PA). 13,20 The skin disks and diffusion cells were perfused with filtered HHBSS receptor fluid (pH = 7.4) at a flow rate of *1.5 mL/h for the duration of the study. 14 Receptor fluid was collected using a fraction collector (FC204; Gilson, Middleton, WI) at 6-hour intervals for a total of 24 hours after dosing.…”

Section: Percutaneous Absorption Experimentsmentioning

confidence: 99%

“…Skin disks (17 mm disks) were mounted dermal side down into flow-through diffusion cells. 13,20 Skin surface temperature was maintained at 32°C. Skin was perfused with HHBSS, pH 7.4.…”

Section: Radiolabeled Dendrimer-enhanced Penetration Studiesmentioning

confidence: 99%

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In Vitro Skin Penetration of Dendrimer Nanoparticles

KraelingMargaret

ToppingVanessa

BelgraveKathleen

et al. 2019

Applied In Vitro Toxicology

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Introduction: Dendrimers are highly branched, stable polymeric nanoparticles with functional groups capable of binding other molecules and may increase delivery of chemicals into skin. Materials and Methods: We examined the skin penetration of generation 3 (G3) to generation 6 (G6) polyamidoamine (PAMAM) amine-terminated (positively charged) dendrimer nanoparticles conjugated with a fluorophore (Alexa Fluor 568) for confocal imaging. The PAMAM dendrimers were further conjugated with succinic anhydride (negative surface charge) or glycidol to impart no charge (neutral). Dendrimers were applied (0.2% concentration) in aqueous solutions or cosmetic formulation onto viable pig or human cadaver skin assembled in diffusion cells. Results: After a 24-hour exposure, most fluorescence appeared in the stratum corneum (SC) or in hair follicles of both pig and human skin. We then examined the skin penetration of radiolabeled generation 4 (G4) dendrimer (0.2% concentration), conjugated with glycidol (OH) and G4 dendrimer bound to glycolic acid (GA), to determine if enhanced skin penetration of GA could occur. The skin penetration of a radiolabeled GA solution and a GA G4-OH dendrimer solution mixture was also evaluated. Most of the radioactive G4-OH dendrimer penetrating human skin remained in the SC (4.7%), with less than 1% absorbing into the epidermis and dermis. When the radiolabeled G4 dendrimer was bound to GA, penetration into SC increased significantly to 29%, and to 4% in the epidermis and dermis compared with the radiolabeled GA treatment alone (4.6%) and the radiolabeled GA G4-OH dendrimer mixture (3.4%). Using radioactivity as a marker, when labeled G4 dendrimer was bound to GA and applied to skin, there was an increase in total skin levels and total penetration levels when compared with GA and GA-dendrimer mixture. Discussion and Conclusion: It appears that the G4 dendrimer may increase the penetration of GA, demonstrating that dendrimer terminal group functionality and charge may alter skin absorption of associated chemicals.

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Section: Maldi-tof Mass Spectrometrymentioning

confidence: 99%

Section: Percutaneous Absorption Experimentsmentioning

confidence: 99%

Section: Percutaneous Absorption Experimentsmentioning

confidence: 99%

Section: Percutaneous Absorption Experimentsmentioning

confidence: 99%

“…Skin disks (17 mm disks) were mounted dermal side down into flow-through diffusion cells. 13,20 Skin surface temperature was maintained at 32°C. Skin was perfused with HHBSS, pH 7.4.…”

Section: Radiolabeled Dendrimer-enhanced Penetration Studiesmentioning

confidence: 99%

See 3 more Smart Citations