In vitro osteolytic activity of human breast carcinoma tissue and prognosis

Dady, P J; Powles, T. J.; Dowsett, Mitch; Easty, G. C.; Williams, J; Neville, A. Munro

doi:10.1038/bjc.1981.31

Cited by 8 publications

(1 citation statement)

References 6 publications

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“…A similar lack of correlation has been reported in patients with breast cancer (Dady et al, 1981) though prognostic significance for raised prostaglandin levels has been claimed by other workers (Fitzpatrick & Stringfellow, 1979).…”

Section: Observations With Carcinoma Cell Linesmentioning

confidence: 69%

Further observations on mechanisms of bone destruction by squamous carcinomas of the head and neck: The role of host stroma

Tsao¹,

Burman²,

Pittam³

et al. 1983

Br J Cancer

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Summary Mechanisms of bone invasion by squamous carcinomas of the head and neck have, been investigated using fresh tumours It is concluded that most squamous cancers of the head and neck are osteolytic in vitro and release a mixture of prostaglandin and non-prostaglandin factors which stimulate osteoclastic bone resorption. These factors are derived from both neoplastic and stromal elements, and are "tumour-associated" rather than "tumour-specific". In vitro bone resorption and prostaglandin release does not correlate with pathological features of the tumour or with post-operative survival.Osteoclasts accumulate at sites of bone invasion by squamous carcinomas of the head and neck, and appear to play an important role in the destructive process (Carter, 1982). Prostaglandins are known stimulants of osteoclastic activity, and raised levels of extractable prostaglandin-like material were demonstrated by bioassay in a series of squamous cancers from the head and neck region (Bennett et al., 1980). Subsequent work showed that fresh tumour tissues and tumour cell lines were osteolytic in vitro and that bone resorption could be partly blocked by indomethacin (Tsao et al., 1981). These studies have been extended and information is now presented on the identification and quantitation of the prostaglandins involved and the contribution made by host tissues as a source of osteolytic factors. Observations on bone resorption by xenografts of squamous carcinomas have been reported separately (Tsao et al., 1983 Materials and methodsBone resorption assay The methods used were based on the procedure devised by Reynolds (1968) and have been fully described by us (Tsao et al., 1981). In brief, calvaria were dissected from 5 to 7 day old BALB/c mice previously injected with 45CaCl2, and cultured on metal grids in modified Bigger's medium, supplemented with heat-inactivated rabbit serum and antibiotics, at 37°C in 5% CO2 in air. Paired half-calvaria were used, one half of each serving as a control. After a preliminary incubation period of 24h to equilibrate calcium exchange between bone and culture medium, the calvaria were cultured for 3 days either with fresh tissue fragnents or with various test and control media-see below. Release of 45Ca was estimated by a liquid scintillation system. The percentage of isotope released from each bone was calculated and osteolysis was expressed in a standard manner as the ratio of the % of 45Ca release from test and control cultures. The values of each bone resorption ratio were recorded as the mean + s.e. of 4 pairs of bone cultures.In vitro osteolysis by fresh tissues Twenty-nine squamous carcinomas were obtained

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Section: Observations With Carcinoma Cell Linesmentioning

confidence: 69%