In Vitro Ortamda Kalsiyum Klorürün Osteoblast Benzeri Hücreler Üzerine Etkisi

Abstract

The aim of the study was to determine whether calcium chloride affects the proliferation of osteoblast like cells in a sex-dependent manner, as well as to determine the most effective concentration on proliferation of osteoblast like cells, in in vitro conditions. Bone marrow was used as biological material from young adult rats, both sexes, aged 90-95 days. Six different concentrations of calcium chloride were tested, determining the numerical representation of osteoblast like cells after 24 and 48 hours. Test results of mean values between males and females after 24 hours, indicate significant differences with a probability of P<0.05 at calcium chloride concentrations of: 0.25 mM and 1.8 mM. Results after 48 hours showed that there were no significant differences at most CaCl2 concentrations. It was found that male osteoblast like cells show a higher affinity for different calcium chloride concentrations when compared to the female osteoblast like cells. The calcium chloride concentration of 0.25 mM affected the proliferation of osteoblast like cells most favorably, which is 26.6% higher than the control values.