In vitro organogenesis from leaf and transverse thin cell layer derived callus cultures of Talinum triangulare (Jacq.) Willd.

Swarna, J.; Ravindhran, R.

doi:10.1007/s10725-012-9780-5

Cited by 14 publications

(7 citation statements)

References 39 publications

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“…(2018) report, it was observed that vascular zone cells have a high tendency to generate new shoot buds and the regeneration capacity of explants depends on nutrient transport across media to tTCL, which has more exposed cells on media as compared to conventional large-size explants. Similar studies have also been reported in Talinum triangulare , Bacteris gasipaes , and Dendrobium candidum ( Steinmacher et al., 2007 ; Zhao et al., 2007 ; Swarna and Ravindhran, 2013 ). According to the literature, TCL explants have a more significant reproducing tendency than conventional explants ( Da Silva and Dobránszki, 2015 ).…”

Section: Discussionsupporting

confidence: 87%

Enhancing drought resistance in Pogostemon cablin (Blanco) Benth. through overexpression of ACC deaminase gene using thin cell layer regeneration system

Warsi,

Khatoon,

Singh

et al. 2023

Front. Plant Sci.

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Pogostemon cablin cultivation faces massive constraints because of its susceptability to drought stress that reduces patchouli propagation and oil yield. The present study has achieved an efficient and rapid direct regeneration system for the transgenic production of P. cablin using Agrobacterium-mediated genetic transformation. To establish an efficient regeneration protocol for fast in-vitro multiplication of patchouli plants, leaf, petiole, and transverse thin cell layer (tTCL) explants were used and inoculated on an MS medium supplemented with different combinations of phytohormones. A comparative study showed a maximum regeneration frequency of 93.30 ± 0.56% per explant was obtained from leaf segments on optimal MS medium fortified with 0.2mg/L BAP and 0.1mg/L NAA. Leaf and petiole explants took 25-35 days to regenerate while tTCL section showed regeneration in just 15-20 days on the same medium. Subsequently, productive genetic transformation protocol OD600 0.6, AS 200µM, 30mg/L kanamycin, and infection time 5 min. was standardized and best-suited explants were infected at optimum conditions from the Agrobacterium tumefaciens (LBA 4404) strain harboring ACC deaminase to generate transgenic P. cablin Benth. (CIM-Samarth) plants. The investigation suggested that the optimized protocol provides a maximum transformation frequency of 42 ± 1.9% in 15-20 days from tTCL. The transgenic plants were shifted to the greenhouse with a 52.0 ± 0.8% survival frequency. A molecular docking study confirmed significant binding affinity of ligand ACC with ACC deaminase at the catalytic site, and ligand interactions showed four H-bonds at the binding pocket with amino acids Cys-196, Val-198, Thr-199, and Gly-200 that validate gene relative expression in transgenic plants. Among all transgenic acclimatized greenhouse-grown patchouli plants, line PT4 showed improved drought resistance under severe water stress as its RWC was 71.7 ± 2.3% to 75.7 ± 2.1% which is greater than the RWC of the control plant, 58.30 ± 0.21%. Analysis of the other physiological indicators, H2O2, chlorophyll content, and ROS result support drought resistance ability. Our study concluded that the first report on P. cablin, tTCL direct regeneration, and standardized transformation protocol created a new opportunity for genetic manipulation to achieve drought-resistant patchouli plants for cultivation in all seasons at the commercial level.

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Section: Discussionsupporting

confidence: 87%

Enhancing drought resistance in Pogostemon cablin (Blanco) Benth. through overexpression of ACC deaminase gene using thin cell layer regeneration system

Warsi,

Khatoon,

Singh

et al. 2023

Front. Plant Sci.

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“…[62] In Asia, the plant is found widely in Sri Lanka and India, especially in Tamil Nadu. [63,64] Nigeria is a rich geographical source of the Talinum genus. [49,65] It is widely distributed in various geographical regions of Asia, East Asia, and Southern America.…”

Section: Morphological Taxonomic Description and Geographical Distrib...mentioning

confidence: 99%

“…[80] Effective plant regeneration can be done for tissue culture applications using shoot explants, [64] internode and leaf-derived callus in MS medium. [63] The presence of betalain in the flowers makes the plant a potential industrial source of natural food colourant. [81] The plant's leaf extract is effective in the green synthesis of silver nanoparticles with enhanced antimicrobial and antioxidative properties.…”

Section: Production and Industrial Usesmentioning

confidence: 99%

“…The T. triangulare is native to tropical Africa, [59,60] and it is cultivated widely in different areas of Western Africa [61] and tropical America [62] . In Asia, the plant is found widely in Sri Lanka and India, especially in Tamil Nadu [63,64] . Nigeria is a rich geographical source of the Talinum genus [49,65] .…”

Section: Morphological Taxonomic Description and Geographical Distrib...mentioning

confidence: 99%

“…In addition, different physical and chemical treatments help break dormancy but provide a varying rate of germination [80] . Effective plant regeneration can be done for tissue culture applications using shoot explants, [64] internode and leaf‐derived callus in MS medium [63] . The presence of betalain in the flowers makes the plant a potential industrial source of natural food colourant [81] .…”

Section: Production and Industrial Usesmentioning

confidence: 99%

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A Review on Bioactive Compounds, Ethnomedicinal Importance and Pharmacological Activities of Talinum triangulare (Jacq.) Willd

Dinesh,

Kumar

2023

Chemistry & Biodiversity

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Talinum triangulare (Jacq.) Willd. is a traditional leafy vegetable used by tribal communities for ethnomedicinal and ethnoculinary preparations. This article reviews the current knowledge of its multiple uses, including pharmacological activities and nutritional composition. The literature survey shows that it has been traditionally useful in the treatment of several diseases, such as anaemia, diabetes, measles, and ulcers and the preparation of various traditional foods. Analysis of the literature on its phytochemicals shows its richness in bioactive compounds. Further, research also shows that this plant has antidiabetic, antiobesity, antitumor, antiulcer, hepatoprotective, and neuroprotective activities besides anti‐inflammatory and antioxidant properties. Nutrient analysis of the plant reveals the presence of Ca, Zn, Fe, vitamins C and E, dietary fibre and protein in considerable quantities. The results of the pharmacological studies on the antidiabetic, antiulcer and anti‐anaemic activities provide support in favour of its ethnomedicinal uses. The presence of bioactive compounds and pharmacological activities show the usefulness of this plant as a functional food.

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The role of crassulacean acid metabolism induction in plant adaptation to water deficit

Habibi

2016

Water Stress and Crop Plants

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In vitro organogenesis from leaf and transverse thin cell layer derived callus cultures of Talinum triangulare (Jacq.) Willd.

Cited by 14 publications

References 39 publications

Enhancing drought resistance in Pogostemon cablin (Blanco) Benth. through overexpression of ACC deaminase gene using thin cell layer regeneration system

Enhancing drought resistance in Pogostemon cablin (Blanco) Benth. through overexpression of ACC deaminase gene using thin cell layer regeneration system

A Review on Bioactive Compounds, Ethnomedicinal Importance and Pharmacological Activities of Talinum triangulare (Jacq.) Willd

The role of crassulacean acid metabolism induction in plant adaptation to water deficit

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