In vitro optimization of retinoic acid–induced neuritogenesis and TH endogenous expression in human SH-SY5Y neuroblastoma cells by the antioxidant Trolox

Frota, Mario Luiz Conte da; Pires, André Simões; Zeidán-Chuliá, Fares; Bristot, Ivi Juliana; Lopes, Fernanda Machado; Pasquali, Matheus Augusto de Bittencourt; Zanotto-Filho, Alfeu; Behr, Guilherme Antônio; Klamt, Fábio; Gelain, Daniel Pens; Moreira, José Cláudio Fonseca

doi:10.1007/s11010-011-0983-2

Cited by 24 publications

(13 citation statements)

References 52 publications

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“…Unexpectedly, Trolox failed to restore the phosphorylation of IGF-1Rs. In agreement with a previous observation [22], the regulation of phosphoAkt was found underlying the protective effect of Trolox in our system ( Fig. 3a and b).…”

Section: Discussionsupporting

confidence: 94%

Hydrogen peroxide attenuates the prosurvival signaling of insulin-like growth factor-1 through two pathways

Sun¹,

Wang²,

Zheng³

2012

NeuroReport

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Although it has been well established that oxidative stress triggering a variety of signaling pathways leads to cell death, little attention has been paid to how these pathways affect prosurvival factors such as insulin-like growth factor-1 (IGF-1). In this study, we found that the prosurvival signaling of IGF-1 was attenuated by H₂O₂. To study the mechanism underlying this phenomenon, cells pretreated with Trolox or various glutamate receptor antagonists [i.e. N-methyl-D-aspartate (NMDA) receptor antagonist dizocilpine maleate (MK-801), non-NMDA receptor antagonist 6,7-dinitroquinoxaline-2,3-dione (DNQX), metabolic glutamate receptor antagonists LY341495 and CPCCOEt] were exposed to H₂O₂, and then stimulated by IGF-1. The phosphorylation statuses of IGF-1 receptors, Akt and ERK, were determined by western blotting, and cell viability was analyzed by an MTT assay. IGF-1 exerted a potent neuroprotective effect against B27 deprivation, and this effect was abolished by 100 μM H₂O₂. Meanwhile, the phosphorylation of IGF-1 receptors, Akt and ERK, was attenuated. Moreover, the phosphorylation of Akt was more susceptible to H₂O₂ insult than IGF-1 receptors. MK-801 increased the phosphorylation of IGF-1 receptors and its downstream target Akt, and thereby promoted cell survival, whereas the other glutamate receptor antagonists exerted no effect. Antioxidant Trolox did not restore IGF-1 signaling, but it increased Akt phosphorylation and also increased cell viability. These results showed that H₂O₂ impaired IGF-1 prosurvival signaling through two pathways. One pathway disrupted the autophosphorylation of IGF-1 receptors through NMDA receptors and the other directly dephosphorylated Akt.

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Section: Discussionsupporting

confidence: 94%

Hydrogen peroxide attenuates the prosurvival signaling of insulin-like growth factor-1 through two pathways

Sun¹,

Wang²,

Zheng³

2012

NeuroReport

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“…The assay is based on the employment of a peroxyl radical generator (2,2-azo-bis(2-amidinopropane); AAPH) mixed with luminol, and the scavenging activity of samples prevents luminol oxidation by AAPH. The synthetic antioxidant Trolox (Acros Organics BVBA, Geel, Belgium), a vitamin E analog, was applied as a positive control at a concentration of 100 µM [38]. The antioxidant capacity of samples was recorded through 60 min and results were calculated as area under the curve (AUC).…”

Section: Methodsmentioning

confidence: 99%

Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats

et al. 2017

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Exercise training intensity is the major variant that influences the relationship between exercise, redox balance, and immune response. Supplement intake is a common practice for oxidative stress prevention; the effects of vitamin A (VA) on exercise training are not yet described, even though this molecule exhibits antioxidant properties. We investigated the role of VA supplementation on redox and immune responses of adult Wistar rats subjected to swimming training. Animals were divided into four groups: sedentary, sedentary + VA, exercise training, and exercise training + VA. Over eight weeks, animals were submitted to intense swimming 5 times/week and a VA daily intake of 450 retinol equivalents/day. VA impaired the total serum antioxidant capacity acquired by exercise, with no change in interleukin-1β and tumor necrosis factor-α levels. In skeletal muscle, VA caused lipid peroxidation and protein damage without differences in antioxidant enzyme activities; however, Western blot analysis showed that expression of superoxide dismutase-1 was downregulated, and upregulation of superoxide dismutase-2 induced by exercise was blunted by VA. Furthermore, VA supplementation decreased anti-inflammatory interleukin-10 and heat shock protein 70 expression, important factors for positive exercise adaptations and tissue damage prevention. Our data showed that VA supplementation did not confer any antioxidative and/or protective effects, attenuating exercise-acquired benefits in the skeletal muscle.

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“…The resulting cellular suspension was centrifuged at 3000 ×g for 10 min, and the supernatant was collected. Cellular superoxide dismutase (SOD) activity was assessed by quantifying the inhibition of superoxide-dependent adrenaline autooxidation in a spectrophotometer at 480 nm, as previously described [12]. Results were expressed as units of SOD/mg protein.…”

Section: Methodsmentioning

confidence: 99%

“…Results were expressed as units of SOD/mg protein. Cellular catalase (CAT) activity was determined by measuring the rate of decrease in H 2 O 2 absorbance in a spectrophotometer at 240 nm [12]. CAT activity was expressed as units of CAT/mg protein.…”

Section: Methodsmentioning

confidence: 99%

Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production

Zeidán-Chuliá

Gelain

Kolling

et al. 2013

Oxidative Medicine and Cellular Longevity

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Scope. To elucidate the morphological and biochemical in vitro effects exerted by caffeine, taurine, and guarana, alone or in combination, since they are major components in energy drinks (EDs). Methods and Results. On human neuronal SH-SY5Y cells, caffeine (0.125–2 mg/mL), taurine (1–16 mg/mL), and guarana (3.125–50 mg/mL) showed concentration-dependent nonenzymatic antioxidant potential, decreased the basal levels of free radical generation, and reduced both superoxide dismutase (SOD) and catalase (CAT) activities, especially when combined together. However, guarana-treated cells developed signs of neurite degeneration in the form of swellings at various segments in a beaded or pearl chain-like appearance and fragmentation of such neurites at concentrations ranging from 12.5 to 50 mg/mL. Swellings, but not neuritic fragmentation, were detected when cells were treated with 0.5 mg/mL (or higher doses) of caffeine, concentrations that are present in EDs. Cells treated with guarana also showed qualitative signs of apoptosis, including membrane blebbing, cell shrinkage, and cleaved caspase-3 positivity. Flow cytometric analysis confirmed that cells treated with 12.5–50 mg/mL of guarana and its combinations with caffeine and/or taurine underwent apoptosis. Conclusion. Excessive removal of intracellular reactive oxygen species, to nonphysiological levels (or “antioxidative stress”), could be a cause of in vitro toxicity induced by these drugs.

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In vitro optimization of retinoic acid–induced neuritogenesis and TH endogenous expression in human SH-SY5Y neuroblastoma cells by the antioxidant Trolox

Cited by 24 publications

References 52 publications

Hydrogen peroxide attenuates the prosurvival signaling of insulin-like growth factor-1 through two pathways

Hydrogen peroxide attenuates the prosurvival signaling of insulin-like growth factor-1 through two pathways

Vitamin A Oral Supplementation Induces Oxidative Stress and Suppresses IL-10 and HSP70 in Skeletal Muscle of Trained Rats

Major Components of Energy Drinks (Caffeine, Taurine, and Guarana) Exert Cytotoxic Effects on Human Neuronal SH-SY5Y Cells by Decreasing Reactive Oxygen Species Production

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