In vitro oocyte fertilization and subsequent embryonic development after cryopreservation of bovine ovarian tissue, using an effective approach for oocyte collection

Faheem, Marwa; Carvalhais, I.; Chaveiro, A.; Silva, Fernando Moreira da

doi:10.1016/j.anireprosci.2011.02.011

Cited by 16 publications

(18 citation statements)

References 24 publications

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“…These results are in agreement with our previous finding using DMSO as a cryoprotectant for ovarian tissue cryopreservation (Faheem et al, 2011), and stating that the impact of the cryopreservation process did not exceed the maturation stage and that the oocytes able to be matured after cryopreservation had the same competence to develop in vitro as fresh matured oocytes. Moreover, when calculated from the survived oocyte numbers, the same trend of embryonic development was observed between the fresh oocytes and the oocytes derived from the PROH-treated group, while the DMSO-treated showed significant difference at the morula stage compared with the fresh oocyte group.…”

Section: Discussionsupporting

confidence: 94%

The effect of vitrification of immature bovine oocytes to the subsequentin vitrodevelopment and gene expression

Faheem

Baron

Carvalhais

et al. 2014

Zygote

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Immature bovine oocytes were vitrified using the cryotop method and their post-warming survivability and capability to undergo in vitro maturation, fertilization and subsequent embryonic development were evaluated. In addition throughout the embryonic 2-cell, 4-cell, morula and blastocyst stages, the expression of four developmentally important genes (Cx43, CDH1, DNMT1 and HSPA14) was analysed using the real-time polymerase chain reaction (PCR). Immature oocytes (n = 550) were randomly assigned to non-vitrified (fresh) or cryotop vitrification groups using ethylene glycol (EG) with 1,2 propanediol (PROH) or dimethylsulphoxide (DMSO). After warming, oocytes survivability, embryo cleavage and embryonic developmental rates were not statistically different between the two cryoprotectants groups. However, the DMSO group had a lower (P < 0.05) oocyte maturation rate compared with the fresh and PROH groups. For morula and blastocyst rates, the DMSO group achieved a lower (P < 0.05) morula rate compared with the fresh group, while at the blastocyst stage, there were no differences between fresh and both cryoprotectants groups. For molecular analysis, at the 4-cell stage, most studied genes showed an inconsistent pattern of expression either from the PROH or DMSO groups. Noteworthily, these differences were limited at the morula and blastocyst stages. In conclusion, the cryotop method is sufficient for vitrification of immature bovine oocytes, both for embryonic developmental competence and at the molecular level. Moreover, PROH showed some advantage over DMSO as a cryoprotectant.

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Section: Discussionsupporting

confidence: 94%

The effect of vitrification of immature bovine oocytes to the subsequentin vitrodevelopment and gene expression

Faheem

Baron

Carvalhais

et al. 2014

Zygote

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“…A population of antral follicles already exists in the ovarian tissue and healthy immature oocytes can be retrieved after cryopreservation and matured in vitro for IVF, as previously reported in the sheep and in the cow (Al‐aghbari and Menino 2002; Faheem et al. 2011).…”

Section: Introductionmentioning

confidence: 83%

“…In sheep, oocytes were successfully cryopreserved by vitrification of ovarian tissues, and they exhibited a maturation rate similar to that of vitrified and non‐vitrified oocytes (Al‐aghbari and Menino 2002). More recently, bovine oocytes recovered from vitrified ovarian tissue showed lower maturation rate than oocytes from fresh tissue, although they developed up to the blastocyst stage after IVF with similar rate (Faheem et al. 2011).…”

Section: Discussionmentioning

confidence: 99%

Effect of Vitrification of Feline Ovarian Cortex on Follicular and Oocyte Quality and Competence

Luvoni

Tessaro

Apparício

et al. 2011

Reprod Domestic Animals

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Cryopreservation of ovarian cortex has important implications in the preservation of fertility and biodiversity in animal species. Slow freezing of cat ovarian tissue resulted in the preservation of follicular morphology and in the follicular development after xenografting. Vitrification has been recently applied to ovarian tissues of different species, but no information is available on the effect of this method on feline ovarian cortex. Moreover, meiotic competence of fully grown oocytes isolated from cryopreserved tissue has not been reported. The aim of this study was to evaluate the effect of vitrification of feline ovarian cortex on follicular morphology and oocyte integrity, as well as meiotic competence. A total of 352 fragments (1.5-2 mm(3) ) were obtained from ovarian cortical tissues: 176 were vitrified and 176 were used fresh as control. Histological evaluation of fresh and vitrified fragments showed intact follicles after cryopreservation procedures with no statistically significant destructive effect from primordial to antral follicles. After IVM, oocytes collected from vitrified ovarian fragment showed a higher proportion of gametes arrested at germinal vesicle (GV) stage compared to those isolated from fresh control tissue (33.8% vs 2.9%; p < 0.001). However, oocytes isolated from vitrified tissues were able to resume meiosis, albeit at lower rate than those collected from fresh tissues (39.8% vs 85.9%; p < 0.00001). Vitrification induced changes in the organization of cytoskeletal elements (actin microfilaments and microtubules) of oocytes, but significantly only for actin network (p < 0.001). Finally, chromatin configuration within the GV was not affected by the cryopreservation procedure. Our study demonstrated that vitrification preserves the integrity of ovarian follicles and that oocytes retrieved from cryopreserved tissue maintain the capability of resuming meiosis. To our knowledge, this has not previously been reported in the cat.

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“…Results published in a previous experiment (Faheem et al. ) stated that more morphologically normal oocytes could be harvested after puncture of antral follicles from post‐thawing ovarian tissue either with slow freezing or with vitrification. Moreover, the capability of these oocytes to undergo normal in vitro fertilization and subsequent embryonic development showed competency and comparable result with the oocytes from fresh ovarian tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The ovarian tissues (n = 45 slices) were vitrified according to Zhou et al (2010) and Faheem et al (2011). Briefly, ovarian slices were rinsed in TCM-199 medium with HEPES, supplemented with 7.5% ethylene glycol (EG), 7.5% dimethyl sulphoxide (DMSO) and 20% foetal bovine serum (FBS, Fr.…”

Section: Ovarian Tissue Vitrification and Thawingmentioning

confidence: 99%

Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles

Faheem

Carvalhais

Baron

et al. 2013

Reprod Domestic Animals

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This study was performed to evaluate the structural preservation of antral follicles after bovine ovarian tissue vitrification using histological analysis. Ovaries (n = 30) of slaughtered cows were cut into small fragments using a scalpel blade, and the ovarian tissues were randomly assigned to vitrification using 15% dimethyl sulphoxide (DMSO) and 15% ethylene glycol (EG) and fresh tissues (control) groups. For histological evaluations, fresh and post-thawing ovarian tissues were immediately fixed, serially sectioned into 5-μm sections and stained with haematoxylin and eosin (H&E). Nine serial sections per fragment were subjected for morphological assessment. The diameter of the antral follicles was determined and classified into four groups: 1 (≤1 mm), 2 (>1-2 mm), 3 (>2-3 mm) and 4 (>3-4 mm). Then, follicular morphology was evaluated in relation to atresia and categorized into seven grades: Grade A (healthy follicle); Grades B, C and D (early atresia); Grades E and F (moderate atresia); and Grade G (advanced atresia). The results revealed that small diameters of antral follicles (1 and 2 mm) were more susceptible for cryoinjury. The normal follicular morphology (Grade A) was not affected by vitrification throughout follicle diameters. Nevertheless, some damage features were monitored after vitrification. In conclusion, the morphological structure of bovine antral follicles could be successfully preserved by ovarian tissue vitrification.

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In vitro oocyte fertilization and subsequent embryonic development after cryopreservation of bovine ovarian tissue, using an effective approach for oocyte collection

Cited by 16 publications

References 24 publications

The effect of vitrification of immature bovine oocytes to the subsequentin vitrodevelopment and gene expression

The effect of vitrification of immature bovine oocytes to the subsequentin vitrodevelopment and gene expression

Effect of Vitrification of Feline Ovarian Cortex on Follicular and Oocyte Quality and Competence

Effect of Bovine Ovarian Tissue Vitrification on the Structural Preservation of Antral Follicles

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