In vitro nucleoside specific immune response by lymphocytes from systemic lupus erythematosus.

Morimoto, Chikao; Steinberg, Alfred D.; Schlossman, Stuart F.; Borel, Yves

doi:10.1172/jci110893

Cited by 24 publications

(6 citation statements)

References 29 publications

(29 reference statements)

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“…Although the beneficial effects of LHG were observed, the related bioactive components of these effects were not fully elucidated yet. Nucleobases, nucleosides, and nucleotides, important hydrosoluble bioactive components, possess multiple functions that LHG also demonstrated , such as immuno‐regulation , anti‐oxidation , and anti‐cancer . Due to the remarkable activities of nucleobases, nucleosides, and nucleotides, and their research blank in LHG, it was necessary to comprehensive analysis these compounds.…”

Section: Introductionmentioning

confidence: 99%

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Zhou

Wang

et al. 2015

J of Separation Science

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A rapid and sensitive hydrophilic interaction ultra high performance liquid chromatography coupled with triple-quadrupole linear ion-trap tandem mass spectrometry method was validated for the simultaneous determination of 20 nucleobases, nucleosides, and nucleotides (within 3.5 min), and then was employed to test the functional food of Luo-Han-Guo samples. The analysis showed that the Luo-Han-Guo was rich in guanosine and uridine, but contained trace levels of the other target compounds. Chemometrics methods were employed to identify 40 batches of Luo-Han-Guo samples from different cultivated forms, regions and varieties. Unsupervised hierarchical cluster analysis and principal component analysis were used to classify Luo-Han-Guo samples based on the level of the 20 target compounds, and the supervised learning method of counter propagation artificial neural network was utilized to further separate clusters and validate the established model. As a result, the samples could be clustered into three primary groups, in which correlation with cultivated varieties was observed. The present strategy could be applied to the investigation of other edible plants containing nucleobases, nucleosides, or nucleotides.

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Section: Introductionmentioning

confidence: 99%

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Zhou

Wang

et al. 2015

J of Separation Science

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“…elucidate the cellular basis of antinuclear antibody production (21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35). Many of the earlier studies dealt with immunoglobulin synthesis or proliferative responses of PBMC stimulated by nonspecific mitogens, foreign antigens, or mixed lymphocyte cultures (21)(22)(23)(24)(25)(26)(27)(28)(29).…”

Section: "mentioning

confidence: 99%

IN vitro production of autoantibodies to u1 ribonucleoproteins by peripheral blood mononuclear cells from patients with connective tissue diseases

Ohosone

Akizuki

Hirakata

et al. 1986

Arthritis & Rheumatism

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Antibodies to U1 ribonucleoproteins produced by peripheral blood mononuclear cells from patients with connective tissue diseases were measured by a sensitive enzyme-linked immunosorbent assay and by immunoblotting. Mononuclear cells from patients with high-titer serum antibodies spontaneously started secreting IgG anti-U1 RNP antibodies on the second day after culture. The amount of anti-U1 RNP in the culture supernatants reached maximum level on day 8. The most effective production of anti-U1 RNP by B cells was observed when they were cultured in the presence of T cells and adherent cells. Mononuclear cells from patients without anti-U1 RNP antibodies or from normal subjects did not produce a measurable amount of anti-U1 RNP. Treatment of mononuclear cells by cycloheximide resulted in complete inhibition of anti-U1 RNP secretion, which indicates that antibody in the culture supernatants reflects the active biologic phenomenon in vitro. The methods described should be useful in the study of the cellular mechanisms involved in antinuclear antibody production of connective tissue diseases.

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“…In addition, ASR is rich in all types of amino acids, nucleosides, and nucleobases, in fact, amino acids account for approximately 6.5% of the total chemical composition . As important hydrosoluble bioactive components, nucleosides, nucleobases, and amino acids possess multiple functions such as immunoregulation, anti‐oxidation, anticancer, obesity suppression, and blood pressure reduction . Considering multiple bioactivities and benefits for human health of these compounds, several analytical methods to detect them have been reported to assess the quality of food or medicinal materials .…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of nucleosides, nucleobases, and amino acids in different parts of Angelicae Sinensis Radix by ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry

Yan

Zhu

et al. 2019

J of Separation Science

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A rapid and sensitive ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry method was established and employed to determine 21 nucleosides, nucleobases, and amino acids in 60 samples from different parts of Angelicae Sinensis Radix. The established methods were validated by good linearity (r2 > 0.9937), limits of detection (0.12–77.75 ng/mL), limits of quantitation (0.31–272.13 ng/mL), intra‐ and interday precisions (RSD ≤ 4.84%, RSD ≤ 6.26%), stability (RSD ≤ 5.92%), repeatability (RSD ≤ 7.14%), recovery (91.4–103.4%), and matrix effects (0.92–1.03). Chemical comparative analysis revealed that the content of total analytes in four parts of Angelicae Sinensis Radix were different, and exhibited the order: Head (14.89 mg/g) > Body (10.15 mg/g) > All (8.22 mg/g) > Tail (6.23 mg/g). Principal component analysis showed that the samples could be classified into four groups in accord with four different parts of Angelicae Sinensis Radix. The results could provide a scientific basis and reference for the quality control of Angelicae Sinensis Radix, and may be conducive to further research on the pharmacological activities of Angelicae Sinensis Radix.

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In vitro nucleoside specific immune response by lymphocytes from systemic lupus erythematosus.

Cited by 24 publications

References 29 publications

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

Chemometrics for comprehensive analysis of nucleobases, nucleosides, and nucleotides in Siraitiae Fructus by hydrophilic interaction ultra high performance liquid chromatography coupled with triple‐quadrupole linear ion‐trap tandem mass spectrometry

IN vitro production of autoantibodies to u1 ribonucleoproteins by peripheral blood mononuclear cells from patients with connective tissue diseases

Comparative analysis of nucleosides, nucleobases, and amino acids in different parts of Angelicae Sinensis Radix by ultra high performance liquid chromatography coupled to triple quadrupole tandem mass spectrometry

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