In vitro non-thermal oxidative stress response after 1800 MHz radiofrequency radiation

Abstract

In this study possible connection between radiofrequency exposure (RF) and development of oxidative stress was investigated by measuring impairment in cellular oxidation-reduction balance immediately after RF exposure. Fibroblast cells V79 were exposed for 10, 30 and 60 minutes to 1800 MHz RF radiation. Electric field strength was 30 V/m and specific absorption rate (SAR) was calculated to be 1.6 W/kg. Electromagnetic field was generated within Gigahertz Transversal Electromagnetic Mode cell (GTEM) equipped by signal generator, amplifier and modulator. Cell viability was determined by CCK-8 colorimetric assay and level of reactive oxygen species (ROS) was detected by dihydroethidium staining. Reduced glutathione (GSH) and glutathione peroxidase (GSH-Px) were used to assess cell antioxidant activity while lipid oxidative damage was evaluated measuring concentration of malondialdehyde. Viability of V79 cells remained within normal physiological values regardless of exposure time. Increased level of superoxide radicals was detected after 60-min exposure. Significantly higher GSH level was observed immediately after 10-min exposure with higher but insignificant activity of GSH-Px. Lipid oxidative damage in exposed cell samples was not observed. Short-term RF exposure revealed transient oxidation-reduction imbalance in fibroblast cells following adaptation to applied experimental conditions.

Analysis of global DNA methylation changes in human keratinocytes immediately following exposure to a 900 MHz radiofrequency field

“…Collectively, this indicates that the observed effect on DNA methylation and gene expression is not associated with bulk heating of the sample. This data is in line with previous studies that showed biological effects in the absence of significant heat [Velizarov et al, 1999; Yu and Yao 2010; IARC, 2013; Loos et al, 2013; Marjanovic Cermak et al, 2017; Belpomme et al, 2018; Halgamuge et al, 2020; Sharma et al, 2022].…”

Manmade Electromagnetic Fields and Oxidative Stress—Biological Effects and Consequences for Health

“…This notion is further supported by observations made in hamster (V79) and human (HSF) fibroblasts. Without having a negative effect on viability or leading to cell damage, exposure to 1.8 GHz RF-EMF (SAR: 1.6/3 W/kg, GSM signal or carrier wave) led to an early transient increase in ROS formation which ceased after 24 h of exposure [113,210,211]. In accordance with these conclusions, no evidence for oxidative DNA damage was found in pulmonary fibroblasts, regardless of the exposure duration (1, 4, 24 h) with different doses (SAR: 0.5, 2, 4.9 W/kg) and modulations of 1.95 GHz RF-EMFs (GSM, UMTS, WiFi) [212].…”

The effect of exposure to 1800 MHz radiofrequency radiation on epidermal growth factor, caspase-3, Hsp27 and p38MAPK gene expressions in the rat eye

“…High-frequency EMF from mobile phones and other modern devices has the potential to damage eye tissues; however, its impact on the gene expression levels of some proteins is undefi ned at present. Investigations showed that RF fi elds may cause alterations in the conformation of cellular proteins and the eventual synthesis of stress response proteins (13,28,29). In this study, we investigated whether 1800 MHz RF-EMF alters Hsp27, p38MAPK, EGFR and caspase-3 gene expression levels or not in the rat eye tissue.…”