In Vitro Neuronal Depolarization And Increased Synaptic Activity Induced By Infrared Neural Stimulation

Entwisle, Blake; McMullan, Simon; Bokiniec, Phillip; Gross, Simon; Chung, Roger S.; Withford, Michael J.

doi:10.1364/fio.2016.jth2a.181

Cited by 2 publications

(2 citation statements)

References 13 publications

(11 reference statements)

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“…Infrared laser pulses have been shown to induce intracellular calcium transients implicating mitochondria in neonatal cardiomyocytes [10] and in neonatal spiral and vestibular ganglion neurons [11] and to depolarize membranes in HEK293 cells [12], dorsal root ganglion neurons [13], oocytes, HEK cells and artificial layers [14], retinal and vestibular primary neurons [15,16], hippocampal neurons [17], spiral ganglion neurons [18], brain slices [19] and in vestibular hair cells and afferent neurons [20]. What remains unclear is whether an universal photothermal mechanism exists and how the transient heating induced by the IR laser pulse elicits membrane depolarization of neurons and action potentials or modulates intracellular signalling.…”

Section: Introductionmentioning

confidence: 99%

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Paris¹,

Marc²,

Charlot³

et al. 2017

Biomed. Opt. Express

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This work focuses on the optical stimulation of dorsal root ganglion (DRG) neurons through infrared laser light stimulation. We show that a few millisecond laser pulse at 1875 nm induces a membrane depolarization, which was observed by the patch-clamp technique. This stimulation led to action potentials firing on a minority of neurons beyond an energy threshold. A depolarization without action potential was observed for the majority of DRG neurons, even beyond the action potential energy threshold. The use of ruthenium red, a thermal channel blocker, stops the action potential generation, but has no effects on membrane depolarization. Local temperature measurements reveal that the depolarization amplitude is sensitive to the amplitude of the temperature rise as well as to the time rate of change of temperature, but in a way which may not fully follow a photothermal capacitive mechanism, suggesting that more complex mechanisms are involved.

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Section: Introductionmentioning

confidence: 99%

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Paris¹,

Marc²,

Charlot³

et al. 2017

Biomed. Opt. Express

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show abstract

“…This appears to be the result of thermally-mediated modulation of mitochondrial calcium cycling (Paviolo et al, 2014), although a non-thermal origin has also been proposed (Golovynska et al, 2019). It is currently unclear what (if any) relation intracellular calcium release has to membrane depolarization, but numerous potential mechanisms have been suggested, most notably synaptic vesicle release (Liu et al, 2014;Entwisle et al, 2016).…”

Section: Infrared Neural Modulationmentioning

confidence: 99%

Nanoparticle-based optical interfaces for retinal neuromodulation: a review

Stoddart,

Begeng,

Tong

et al. 2024

Front. Cell. Neurosci.

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Degeneration of photoreceptors in the retina is a leading cause of blindness, but commonly leaves the retinal ganglion cells (RGCs) and/or bipolar cells extant. Consequently, these cells are an attractive target for the invasive electrical implants colloquially known as “bionic eyes.” However, after more than two decades of concerted effort, interfaces based on conventional electrical stimulation approaches have delivered limited efficacy, primarily due to the current spread in retinal tissue, which precludes high-acuity vision. The ideal prosthetic solution would be less invasive, provide single-cell resolution and an ability to differentiate between different cell types. Nanoparticle-mediated approaches can address some of these requirements, with particular attention being directed at light-sensitive nanoparticles that can be accessed via the intrinsic optics of the eye. Here we survey the available known nanoparticle-based optical transduction mechanisms that can be exploited for neuromodulation. We review the rapid progress in the field, together with outstanding challenges that must be addressed to translate these techniques to clinical practice. In particular, successful translation will likely require efficient delivery of nanoparticles to stable and precisely defined locations in the retinal tissues. Therefore, we also emphasize the current literature relating to the pharmacokinetics of nanoparticles in the eye. While considerable challenges remain to be overcome, progress to date shows great potential for nanoparticle-based interfaces to revolutionize the field of visual prostheses.

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In Vitro Neuronal Depolarization And Increased Synaptic Activity Induced By Infrared Neural Stimulation

Cited by 2 publications

References 13 publications

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Millisecond infrared laser pulses depolarize and elicit action potentials on in-vitro dorsal root ganglion neurons

Nanoparticle-based optical interfaces for retinal neuromodulation: a review

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