In vitro multiplication of 'Flordaguard' rootstock: cytokinin source and concentration effects, explants orientation and period of permanence in the culture medium

Radmann, Elizete Beatriz; Bianchi, Valmor João; Fachinello, José Carlos; Ferreira, Letícia Vanni; Oliveira, R. P. de

doi:10.1590/s1516-89132011000100004

Cited by 12 publications

(21 citation statements)

References 17 publications

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“…BAP is responsible for cell division, overcoming apical dominance, and induction of the proliferation of axillary buds (MOK et al, 2000). The present results are similar to those observed by Moraes et al (2004) in Pyrus calleryana D-6 and Radmann et al (2011) in the rootstock 'Flordaguard' (Prunus persica L. Bastch). Both studies found that in the absence of BAP, the emission of new shoots does not take place, creating a dependency on the addition of exogenous cytokinin for the induction of shoots in these two Rosaceae species.…”

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confidence: 87%

In vitro multiplication of pear tree cultivar Cascatense

et al. 2016

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One main obstacle to the development and expansion of pear tree cultivation in Brazil has been the lack of cultivars adapted to the edaphoclimatic conditions of regions with the potential for pear production. Intending to overcome this hindrance, Embrapa Clima Temperado (Embrapa Temperate Climate) developed and released the cultivar Cascatense, with a low cold requirement (~400 h) better suited for Rio Grande do Sul`s and Santa Catarina`s climate. Still, seedlings of high genetic and sanitary quality are necessary for the establishment of a high-yield orchard. Plants with both sanitary quality and uniformity can be obtained through in vitro micropropagation. Thus, the present study aimed to assess the influence of different culture media (MS, MS¾, WPM, QL, and Himédia), concentrations of sucrose (0, 15, 30, 45, and 60 g L -1 ), and concentrations of benzylaminopurine (BAP) (0, 0.4, 0.8, 1.2, and 1.6 mg L -1 ) upon the in vitro multiplication of cultivar Cascatense, to establish an efficient protocol to enhance its propagation and reproducibility. The study included three sequential experiments in which the number of shoots per explant, the length of shoots, the number of axillary buds per shoot, fresh mass, and dry mass of shoots were evaluated. Cultivar Cascatense can be best propagated in vitro with the use of Himédia medium, supplemented with 0.8 mg L ResumoUm dos principais entraves ao desenvolvimento e expansão da cultura da pereira no Brasil tem sido a falta de cultivares adaptadas às condições edafoclimáticas das regiões potencialmente produtoras. Visando sanar esta dificuldade, a Embrapa Clima Temperado desenvolveu e lançou a cv. Cascatense, com baixa exigência em frio (~400 h), mais adequada ao clima do Rio Grande do Sul e de Santa Catarina. No entanto, mudas com alta qualidade genética e sanitária são necessárias para garantir a boa formação de um pomar produtivo. Plantas com qualidade sanitária e uniformidade podem ser obtidas através da micropropagação in vitro. Assim, o presente trabalho teve como objetivo verificar a influência de diferentes meios de cultivo (MS, MS¾, WPM, QL e Himédia), concentrações de sacarose (0, 15, 30, 45 e 60 g L -1 ) e benzilaminopurina -BAP (0; 0,4; 0,8; 1,2 e 1,6 mg L -1 ) na multiplicação in vitro da cv. Cascatense, visando obter um protocolo eficiente para potencializar sua propagação e reprodutibilidade. Os estudos foram conduzidos em três experimentos sequenciais, nos quais foram avaliados o número de brotações por explante, o comprimento das brotações, o número de gemas axilares por brotação, massa fresca e massa seca das brotações. A cv. Cascatense pode ser propagada in vitro com a utilização do meio Himédia suplementado com 0,8 mg L -1 de BAP e 30 g L -1 de sacarose. Palavras-chave: Meio himédia. Sulfato ferroso. Sulfato férrico. Sacarose. Benzilaminopurina.

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confidence: 87%

In vitro multiplication of pear tree cultivar Cascatense

et al. 2016

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“…The reason behind this is that the composition of nutrient media is in agreement with the plant growth requirements. As the shoot multiplication under in vitro condition is largely depends on plant genotype and cytokinins concentration (Radmann et al, 2011) so cytokinins concentration in medium must be optimized in order to get effective shoot proliferation (Khan et al, 2010). According to the results, cultivar "BARI Zaitoon-2" performed better under different cytokinins concentrations by having more shooting percentage (%) and number of shoots per explant.…”

Section: Methodsmentioning

confidence: 99%

Effect of Cytokinins on in Vitro Shoot Proliferation of Olive Cultivars “Earlik” and “Bari Zaitoon-2”

Ashraf¹,

Manzoor²,

Zulfiqar³

et al. 2018

World J. Biol. Biotechnol.

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In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an experiment was designed with an objective to study the effect of different cytokinins (BAP and Zeatin) on shoot proliferation of two olive cultivars (Earlik and BARI Zaitoon-2). In olive Rugini medium, BAP and Zeatin were added alone and in combination at a concentration of 1 mg L-1 and 2 mg L-1. Experiments were arranged according to completely randomized design (CRD) with three replications per treatment. Results showed that both the olive cultivars performed well when BAP + Zeatin were supplemented to the medium at 2+2 mg L-1 concentration. However, the cultivar “BARI Zaitoon-2” had the highest shooting percentage (90.67 %) and number of nodes per shoot (8.33) in treatment containing BAP (2 mg L-1) + Zeatin (2 mg L-1), whereas, “Earlik” at same concentration had more shoot length (9.10 cm) and number of shoots per explant (3.67). Thus, it was concluded that the findings of these results will help in the future for developing shoot proliferation protocols for other olive cultivars. Moreover, these protocols can also be further be used in other woody plants micro propagation

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“…Pérola and Imperial cultivars were provided by the EMBRAPA Tabuleiros Costeiros (Aracaju, SE, Brazil). The micropropagation was carried out using MS medium (Murashige and Skoog 1962) without growth phytoregulator as described by Corrêa et al (2009), Quoirin et al (2008), Radmann et al (2011) and Scherwinski-Pereira et al (2009). The MS medium was supplied with 30 g/L of sucrose and 7 g/L of agar-agar.…”

Section: Micropropagationmentioning

confidence: 99%

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

Leite

Lima

Santana

et al. 2012

Braz. arch. biol. technol.

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This study aimed to obtain the condition of maximum bromalein activity in different parts of pineapple plants produced in vitro, by micropropagation. The sStems and leaves of Pérola and Imperial cultivar plants were evaluated after three and eight months of in vitro cultivation in Murashige and Skoog medium without growth phytoregulator, macerated in potassium phosphate buffer at different pH values (5.7, 6.7 and 7.7). Total protein and proteolytic activity were determined in the samples after three- and eight-month cultivation periods. For both the cultivars, the best results were obtained at pH 5.7 in extraction media. Pérola cultivar, showed higher bromelain activity in the leaves cultivated in vitro for three months (0.0194U/mL) while in the Imperial cultivar, it was higher in the stem after eight months (0.0179 U/mL). Imperial cultivar showed higher bromelain activity than the Pérola's

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In vitro multiplication of 'Flordaguard' rootstock: cytokinin source and concentration effects, explants orientation and period of permanence in the culture medium

Abstract: ABSTRACT

Cited by 12 publications

References 17 publications

In vitro multiplication of pear tree cultivar Cascatense

In vitro multiplication of pear tree cultivar Cascatense

Effect of Cytokinins on in Vitro Shoot Proliferation of Olive Cultivars “Earlik” and “Bari Zaitoon-2”

Determination of optimal condition to obtain the bromelain from pineapple plants produced by micropropagation

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