In Vitro Multiplication of Artichoke and Virus Elimination by Termotherapy and Chemotherapy

Navacchi, O.; Zuccherelli, G.; Zuccherelli, S.

doi:10.17660/actahortic.2005.681.55

Cited by 4 publications

(3 citation statements)

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“…In this technique, diseased in vitro shoots were cultured on an antivirus chemical-containing medium and then subjected to heat treatments, followed by shoot tip culture. In a few cases, diseased in vitro shoots were first heat-treated and then cultured on antivirus chemicals-containing medium, followed by shoot tip culture [ 31 , 32 ]. Although several antivirus chemicals were available against plant viruses, ribavirin was the most frequently used, and sometimes 2-thiouracil was also used, for virus eradication [ 12 , 19 , 23 ].…”

Section: Thermotherapy-based Methods For Virus Eradicationmentioning

confidence: 99%

“…Reduced survival levels of the treated shoots by increased ribavirin concentrations were reported in garlic [ 16 ], pear [ 31 , 76 ], apple [ 44 , 75 ], raspberry [ 76 ] and grapevine [ 77 ] for grapevine. Optimal ribavirin concentration for production of virus-free plants was 12.21 mg L −1 (50 μM used) for lily infected with lily symptomless virus (LSV), tulip breaking virus-lily (TBV-L) and cucumber mosaic virus (CMV) [ 7 ], 20 mg L −1 for potato infected with PLRV and PVY [ 49 , 74 ], Begonia infected with PNRSV [ 68 ], artichoke infected with artichoke latent virus (ALV) [ 32 ] and cassava infected with east African cassava mosaic virus (EACMV), [ 60 ], 25 mg L −1 for apple infected with ACLSV, ASPV and ASGV [ 36 ] and pear infected with ACLSV and ASGV [ 65 ], 50 mg L −1 for pear infected with ACLSV [ 31 ], garlic infected with OYDV, leek yellow stripe virus (LYSV), shallot latent virus (SLV), mite borne filamentous virus (MbFV) and garlic common latent virus (GCLV) [ 16 ].…”

Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

“…Survival levels of the heat-treated shoots and virus free frequencies differed from two apple cultivars by thermotherapy followed by shoot tip culture [ 75 ]. Genotype-specific responses were also found in a number of plants including woody species such as Prunus [ 64 ] and Vitis [ 86 ], and herbaceous species such as Allium sativum [ 55 , 87 ], artichoke infected with ALV [ 32 ], Solanum tuberosum [ 29 ], Lilium [ 56 , 88 ], Cynara cardunculus var. scolymus [ 89 ] and Manihot esculenta [ 59 ].…”

Section: Key Factors Affecting Success Of Virus Eradicationmentioning

confidence: 99%

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In vitro thermotherapy-based methods for plant virus eradication

Wang

Cui

et al. 2018

Plant Methods

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Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vitro techniques represent the most successful approaches for virus eradication. In vitro thermotherapy-based methods, including combining thermotherapy with shoot tip culture, chemotherapy, micrografting or shoot tip cryotherapy, have been successfully established for efficient eradication of various viruses from almost all of the most economically important crops. The present study reviewed recent advances in in vitro thermotherapy-based methods for virus eradication since the twenty-first century. Mechanisms as to why thermotherapy-based methods could efficiently eradicate viruses were discussed. Finally, future prospects were proposed to direct further studies.

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