In vitro morphogenetic responses from cotyledonary explants of immature zygotic embryos of Pterocarpus santalinus

Chakraborty, Tanushree; Chaitanya, K. V.; Lambardi, Maurizio; Akhtar, Nasim

doi:10.1007/s11240-022-02320-6

Cited by 5 publications

(2 citation statements)

References 55 publications

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“…Sucrose is also an important factor for the induction of somatic embryogenesis because it acts as an osmotic stress (Verma et al 2016). The author's group also worked with 3% sucrose on the same explant and resulted in non-embryonic callus, the results are already being published (Chakraborty et al 2022). Our current observations and results are in accordance with Verma et al…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)

Chakraborty

Chaitanya

Akhtar

2023

Plant Cell Tiss Organ Cult

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Cotyledonary segments from the germinated immature zygotic embryos were used for somatic embryogenesis of red sandalwood (Pterocarpus santalinus). It was established on Murashige and Skoog (MS) medium containing 5% sucrose and amalgamation of 6-benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), and α-Naphthaleneacetic acid (NAA). All treatments were responsive for callus induction with the frequency range between 36-97%. The maximum embryogenic frequency (69.44%) was obtained when 0.1 mg/l BAP + 2 mg/l 2,4-D and 0.1 mg/l BAP + 4 mg/l 2,4-D combinations were used. When explants were treated individually with growth regulators, the maximum embryogenic frequency (58.33%) was produced by 4 mg/l 2,4-D. BAP was completely ineffective for somatic embryogenesis when used individually. The average number of globular-staged somatic embryos ranged between 1-5 (irrespective of the treatments). The maximum number of the cotyledonary-staged somatic embryos (2.85) were obtained with treatment 0.1 mg/l BAP and 2 mg/l 2,4-D. The maximum plantlets were developed (1.30) when the cotyledonary-staged embryos from 0.1 mg/l BAP and 2 mg/l 2,4-D were transferred to MS basal medium. The plantlets obtained were acclimatized and showed 100% survival in the greenhouse condition. The embryonic cells have been histologically distinguished from nonembryonic cells with dense cytoplasm and a long suspensor. The induction, maturation and germination of somatic embryos were challenging, suggesting the need for molecular approaches through proteomic expression for mass production and understanding the evolution, structure, and genetic organization of the plant species. Key Message 2,4-D and NAA, either individually or in combination with BAP has a signi cant effect on embryonic potential of cotyledon explant of P. santalinus.

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Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)

Chakraborty

Chaitanya

Akhtar

2023

Plant Cell Tiss Organ Cult

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“…To our knowledge, there is no report of somatic embryogenesis in P. santalinus. Recently in vitro morphogenesis responses is reported from cotyledonary explants of immature zygotic embryos of P. santalinus (Chakraborty et al 2022a) following the analysis of regeneration protocols for micropropagation (Chakraborty et al 2022b). This study documents the regeneration of plantlets by establishment of somatic embryogenesis in red sandalwood and histological analysis to differentiate embryonic and non-embryonic cells from cotyledon explants.…”

Section: Introductionmentioning

confidence: 99%

Somatic Embryogenesis and Plantlet Regeneration in Red Sandalwood (Pterocarpus Santalinus)

Chakraborty

Chaitanya

Akhtar

2022

Preprint

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Cotyledonary segments from the germinated immature zygotic embryo were used for somatic embryogenesis of red sandalwood (Pterocarpus santalinus). It was established on Murashige and Skoog (MS) medium containing 5% sucrose and amalgamation of 6-benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), and α-Naphthaleneacetic acid (NAA). All treatments were responsive for callus induction with the frequency range between 36–97%. The attempts to obtain embryogenesis with 3% sucrose were ineffective since only non-embryonic callus was observed. The maximum frequency of embryogenic induction (69.44%) was obtained in 0.1 mg/l BAP and 2 mg/l 2,4-D; the data was not significantly (p < 0.05) different from the result obtained when 0.1 mg/l BAP and 4 mg/l 2,4-D were used. When explants were treated individually with growth regulators, the maximum (58.33%) frequency was produced by 4 mg/l 2,4-D. The BAP was completely ineffective when used individually. The maximum number of an advanced-stage embryo (2.85) was obtained with treatment 0.1 mg/l BAP and 2 mg/l 2,4-D. The maximum plantlets were developed (1.30) when the cotyledonary-staged embryo from 0.1 mg/l BAP and 2 mg/l 2,4-D were transferred to MS basal medium. The maturation and germination of somatic embryos were challenging, suggesting the need for molecular approaches through proteomic expression for mass production and understanding the evolution, structure, and genetic organization of the plant species. The plantlets obtained were acclimatized and survived in green house condition. Histological observation showed that the cells of the globular embryos had a dense cytoplasm and a long suspensor and used to distinguish between embryonic and non-embryonic cells.

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Effects of Growth Regulators, Organic Nitrogen Sources, Various Nutrient, and Non-Nutrient Adjuvants on Somatic Embryogenesis in Red Sandalwood (Pterocarpus Santalinus)

Chakraborty,

Chaitanya,

Akhtar

2023

J Plant Growth Regul

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In vitro morphogenetic responses from cotyledonary explants of immature zygotic embryos of Pterocarpus santalinus

Cited by 5 publications

References 55 publications

Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)

Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)

Somatic Embryogenesis and Plantlet Regeneration in Red Sandalwood (Pterocarpus Santalinus)

Effects of Growth Regulators, Organic Nitrogen Sources, Various Nutrient, and Non-Nutrient Adjuvants on Somatic Embryogenesis in Red Sandalwood (Pterocarpus Santalinus)

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