In Vitro Models of Angiogenesis

Ucuzian, Areck A.; Greisler, Howard P.

doi:10.1007/s00268-006-0763-4

Cited by 83 publications

(66 citation statements)

References 69 publications

(93 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The culture of endothelial cells within or on top of 3D matrices such as type I collagen, fibrin and Matrigel provides simple, popular models of angiogenesis (Auerbach et al, 2003;Goodwin, 2007;Phung and Dass, 2006;Ucuzian and Greisler, 2007). The present work reports that when blood vascular endothelial cells such as HUVECs and HAECs are cultured under such conditions, they show gene expression changes that are characteristic of a shift to a lymphatic phenotype.…”

Section: Discussionmentioning

confidence: 67%

Reversible transdifferentiation of blood vascular endothelial cells to a lymphatic-like phenotype in vitro

Cooley

Handsley

Zhou

et al. 2010

Journal of Cell Science

View full text Add to dashboard Cite

SummaryBlood vascular cells and lymphatic endothelial cells (BECs and LECs, respectively) form two separate vascular systems and are functionally distinct cell types or lineages with characteristic gene expression profiles. Interconversion between these cell types has not been reported. Here, we show that in conventional in vitro angiogenesis assays, human BECs of fetal or adult origin show altered gene expression that is indicative of transition to a lymphatic-like phenotype. This change occurs in BECs undergoing tubulogenesis in fibrin, collagen or Matrigel assays, but is independent of tube formation per se, because it is not inhibited by a metalloproteinase inhibitor that blocks tubulogenesis. It is also reversible, since cells removed from 3D tubules revert to a BEC expression profile upon monolayer culture. Induction of the lymphatic-like phenotype is partially inhibited by co-culture of HUVECs with perivascular cells. These data reveal an unexpected plasticity in endothelial phenotype, which is regulated by contact with the ECM environment and/or cues from supporting cells.

show abstract

Section: Discussionmentioning

confidence: 67%

Reversible transdifferentiation of blood vascular endothelial cells to a lymphatic-like phenotype in vitro

Cooley

Handsley

Zhou

et al. 2010

Journal of Cell Science

View full text Add to dashboard Cite

show abstract

“…40 Due to their active role in angiogenesis, ECs are a key cell type. 41 Besides that, ECs are pivotal members of a complex interactive communication network in bone. 42 Therefore, the chemical composition as well as architecture of combined structures was designed envisioning not only bone-forming cells but also ECs.…”

Section: Discussionmentioning

confidence: 99%

Design of Nano- and Microfiber Combined Scaffolds by Electrospinning of Collagen onto Starch-Based Fiber Meshes: A Man-Made Equivalent of Natural Extracellular Matrix

Tuzlakoğlu

Santos

Neves

et al. 2011

Tissue Engineering Part A

View full text Add to dashboard Cite

Mimicking the structural organization and biologic function of natural extracellular matrix has been one of the main goals of tissue engineering. Nevertheless, the majority of scaffolding materials for bone regeneration highlights biochemical functionality in detriment of mechanical properties. In this work we present a rather innovative construct that combines in the same structure electrospun type I collagen nanofibers with starchbased microfibers. These combined structures were obtained by a two-step methodology and structurally consist in a type I collagen nano-network incorporated on a macro starch-based support. The morphology of the developed structures was assessed by several microscopy techniques and the collagenous nature of the nanonetwork was confirmed by immunohistochemistry. In addition, and especially regarding the requirements of large bone defects, we also successfully introduced the concept of layer by layer, as a way to produce thicker structures. In an attempt to recreate bone microenvironment, the design and biochemical composition of the combined structures also envisioned bone-forming cells and endothelial cells (ECs). The inclusion of a type I collagen nano-network induced a stretched morphology and improved the metabolic activity of osteoblasts. Regarding ECs, the presence of type I collagen on the combined structures provided adhesive support and obviated the need of precoating with fibronectin. It was also importantly observed that ECs on the nano-network organized into circular structures, a three-dimensional arrangement distinct from that observed for osteoblasts and resembling the microcappillary-like organizations formed during angiogenesis. By providing simultaneously physical and chemical cues for cells, the herein-proposed combined structures hold a great potential in bone regeneration as a man-made equivalent of extracellular matrix.

show abstract

“…Since the formation and maintenance of the vascular network is a complex process, problems related to its regulation are common (Ucuzian and Greisler, 2007). Inadequate vessel maintenance or growth causes ischemia in myocardial infarction and neurodegenerative or obesity-associated disorders, whereas excessive vascular growth or abnormal remodeling promotes cancer, inflammatory disorders and eye diseases (Potente et al, 2011).…”

Section: Introduction #mentioning

confidence: 99%

“…Angiogenesis models are important tools for studying the mechanisms of angiogenesis and the therapeutic strategies to modulate neovascularization (Ucuzian and Greisler, 2007). Due to an increasing amount of compounds affecting the vascular system, accurate vasculogenesis and angiogenesis models are needed for chemical safety testing and for drug development (Sarkanen et al, 2011;Bishop et al, 1999).…”

Section: Introduction #mentioning

confidence: 99%

Human vascular model with defined stimulation medium – a characterization study

Huttala

2015

ALTEX

View full text Add to dashboard Cite

SummaryThe formation of blood vessels is a vital process in embryonic development and in normal physiology. Current vascular modelling is mainly based on animal biology leading to species-to-species variation when extrapolating the results to humans. Although there are a few human cell based vascular models available, these assays are insufficiently characterized in terms of culture conditions and developmental stage of vascular structures. Therefore, well characterized vascular models with human relevance are needed for basic research, embryotoxicity testing, development of therapeutic strategies and for tissue engineering. We have previously shown that the in vitro vascular model based on co-culture of human adipose stromal cells (hASC) and human umbilical vein endothelial cells (HUVEC) is able to induce an extensive vascular-like network with high reproducibility. In this work we developed a defined serum-free vascular stimulation medium (VSM) and performed further characterization in terms of cell identity, maturation and structure to obtain a thoroughly characterized in vitro vascular model to replace or reduce corresponding animal experiments. The results showed that the novel vascular stimulation medium induced an intact and evenly distributed vascular-like network with morphology of mature vessels. Electron microscopic analysis assured the three-dimensional microstructure of the network containing lumen. Additionally, elevated expression levels of the main human angiogenesis-related genes were detected. In conclusion, with the newly defined medium the vascular model can be utilized as a characterized test system for chemical testing as well as in creating vascularized tissue models.

show abstract

In Vitro Models of Angiogenesis

Cited by 83 publications

References 69 publications

Reversible transdifferentiation of blood vascular endothelial cells to a lymphatic-like phenotype in vitro

Reversible transdifferentiation of blood vascular endothelial cells to a lymphatic-like phenotype in vitro

Design of Nano- and Microfiber Combined Scaffolds by Electrospinning of Collagen onto Starch-Based Fiber Meshes: A Man-Made Equivalent of Natural Extracellular Matrix

Human vascular model with defined stimulation medium – a characterization study

Contact Info

Product

Resources

About