In vitro mineralization of hydroxyapatite on electrospun poly(ɛ-caprolactone)–poly(ethylene glycol)–poly(ɛ-caprolactone) fibrous scaffolds for tissue engineering application

Fu, Shaozhi; Yang, Linglin; Fan, Juan; Wen, Qinglian; Lin, Sheng; Wang, Biqiong; Chen, LanLan; Meng, Xiao-Hang; Chen, Yue; Wu, Jingbo

doi:10.1016/j.colsurfb.2013.01.068

Cited by 25 publications

(14 citation statements)

References 36 publications

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“…[32] The electrospinning method has been actively explored recently and offers ultrafine polymer fibers, a high specific surface area, and the possibility of various modifications, including mineralization of scaffolding with HA, which has been shown to reduce cellular cytotoxicity in vitro. [33,34] The features of nanofiber mats are morphologically similar to those of the extracellular matrix of natural tissue.…”

Section: The Bone Healing Process and Nanotechnologymentioning

confidence: 99%

Nanotechnology applications in osteodistraction

Singleton¹,

Halen²

2014

Plast Aesthetic Res

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Most current strategies for bone regeneration have relatively satisfactory results. However, there are drawbacks and limitations associated with their use and availability, and even controversial reports about their efficacy and cost-effectiveness. The induction of new bone formation through distraction osteogenesis (DO) is widespread clinical application in the treatment of bone defects, limb deformities, and fracture nonunions. However, a lengthy period of external fixation is usually needed to allow the new bone to consolidate, and complications such as refracture at the distraction gap often occur. Although various biomaterials have been used as injectable delivery systems in DO models, little has been reported on the use of nanobiomaterials as carrier materials for the sustained release of growth factors in bone regeneration. One area of focus in nanotechnology is the delivery of osteogenic factors in an attempt to modulate the formation of bone. This review article seeks to demonstrate the potential of nanobiomaterials to improve biological applications pertinent to osteodistraction.

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Section: The Bone Healing Process and Nanotechnologymentioning

confidence: 99%

Nanotechnology applications in osteodistraction

Singleton¹,

Halen²

2014

Plast Aesthetic Res

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“…These include non-biodegradable polymers such as polysiloxanes 103 , poly(methyl methacrylate) 104 and other acrylics 105 , polyethylene 106 and polypropylene 107 among olefins and poly(vinyl alcohol) 108 among haloalkanes. Biodegradable polymers used in combination with CAP include hyaluronic acid 109,110 and chitosan 111 as the commonest biological choices, poly(ε-caprolactone) 112,113 as another popular polyester, various polyanhydrides and some of the more rarely used polymers, counting poly- p -dioxanone 114 , poly(trimethylene carbonate) 115 , poly(ethylene oxide terephthalate)-poly(butylene terephthalate) block copolymer 116 , and many others.…”

Section: Polymeric/cap Compositesmentioning

confidence: 99%

When 1 + 1 > 2: Nanostructured composites for hard tissue engineering applications

Uskoković

2015

Materials Science and Engineering: C

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Multicomponent, synergistic and multifunctional nanostructures have taken over the spotlight in the realm of biomedical nanotechnologies. The most prospective materials for bone regeneration today are almost exclusively composites comprising two or more components that compensate for the shortcomings of each one of them alone. This is quite natural in view of the fact that all hard tissues in the human body, except perhaps the tooth enamel, are composite nanostructures. This review article highlights some of the most prospective breakthroughs made in this research direction, with the hard tissues in main focus being those comprising bone, tooth cementum, dentin and enamel. The major obstacles to creating collagen/apatite composites modeled after the structure of bone are mentioned, including the immunogenicity of xenogeneic collagen and continuously failing attempts to replicate the biomineralization process in vitro. Composites comprising a polymeric component and calcium phosphate are discussed in light of their ability to emulate the soft/hard composite structure of bone. Hard tissue engineering composites created using hard material components other than calcium phosphates, including silica, metals and several types of nanotubes, are also discoursed on, alongside additional components deliverable using these materials, such as cells, growth factors, peptides, antibiotics, antiresorptive and anabolic agents, pharmacokinetic conjugates and various cell-specific targeting moieties. It is concluded that a variety of hard tissue structures in the body necessitates a similar variety of biomaterials for their regeneration. The ongoing development of nanocomposites for bone restoration will result in smart, theranostic materials, capable of acting therapeutically in direct feedback with the outcome of in situ disease monitoring at the cellular and subcellular scales. Progress in this research direction is expected to take us to the next generation of biomaterials, designed with the purpose of fulfilling Daedalus’ dream - not restoring the tissues, but rather augmenting them.

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“…In the first method, scaffolds often have low mechanical properties, but in the second one, higher mechanical properties would be gained. Co‐spinning of the suspensions of HA and polymer becomes impossible by increasing the hydroxyapatite content, because the electrospinning process is always blocked . Poly( l ‐lactic) acid is one of the synthetic polymers with high mechanical properties and good processibility, biodegradability and biocompatibility which is mostly used in tissue engineering .…”

Section: Introductionmentioning

confidence: 99%

“…To this aim an aqueous solution with ion concentration similar to natural blood plasma is used which is called simulated body fluid (SBF). This technique is a suitable one to fabricate polymer/ceramic composites . Alternate soaking method forms apatite crystals after the substrates are incubated in solutions containing Ca 2+ and PO 4 3− alternatively for a certain number of cycles .…”

Section: Introductionmentioning

confidence: 99%

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

Ataie

Shabani

Seyedjafari

2018

J Biomedical Materials Res

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Surface mineralized nanofibrous scaffolds hold great potential for bone tissue engineering applications. In this study, a new hybrid nanofibrous scaffold composed of alginate/poly(l‐lactide) nanofibers was fabricated using electrospinning method and then crosslinking process was employed. Hydroxyapatite crystal formation took place using in situ precipitation by immersion of the scaffolds in simulated body fluid solution for 10 days at 37°C. The morphologies of the scaffolds were observed using scanning electron microscope. Hydroxyapatite crystal formation on the surface of nanofibers was confirmed using scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, Fourier transform infrared spectroscopy and X‐ray diffraction analysis. The biocompatibility of the fabricated scaffolds was evaluated using mesenchymal stem cell culture and MTT assay. According to alkaline phosphatase activity and calcium content assays, it was concluded that the osteogenic differentiation of stem cells was considerable on scaffolds containing hydroxyapatite crystals in comparison with other specimens. The results showed biocompatibility of the scaffolds and their support from stem cell adhesion, growth and osteogenic differentiation, so the hydroxyapatite‐coated poly(l‐lactide)/alginate hybrid nanofibrous scaffolds hold suitable characteristics for bone tissue engineering applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 586–596, 2019.

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In vitro mineralization of hydroxyapatite on electrospun poly(ɛ-caprolactone)–poly(ethylene glycol)–poly(ɛ-caprolactone) fibrous scaffolds for tissue engineering application

Cited by 25 publications

References 36 publications

Nanotechnology applications in osteodistraction

Nanotechnology applications in osteodistraction

When 1 + 1 > 2: Nanostructured composites for hard tissue engineering applications

Surface mineralized hybrid nanofibrous scaffolds based on poly(l‐lactide) and alginate enhances osteogenic differentiation of stem cells

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