In vitro mineralization of functional polymers

Kępa, Katarzyna; Coleman, Robert J.; Grøndahl, Lisbeth

doi:10.1016/j.bsbt.2015.09.001

Cited by 54 publications

(38 citation statements)

References 104 publications

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“…The Ca/P molar ratio increases linearly as a function of mineralization cycles, with values of 1.28 ± 0.02 (CHCN1), 1.44 ± 0.03 (CHCN2), and 1.65 ± 0.02 (CHCN3). Therefore, only for the CHCN3 scaffold the Ca/P ratio is close to the theoretical value of 1.67 of the stoichiometric hydroxyapatite . An increase in the number of cycles promotes an increase in the amount of calcium phosphate deposited and Ca/P ratio showed a linear relation with an increase in mineralization cycle.…”

Section: Resultsmentioning

confidence: 99%

“…In order to determine the influence of mineralization cycles number, three different samples were prepared. The mineralization process was performed by alternate immersion method that consists of alternate soaking cycles in two different solutions. Solution 1 was 0.12 mol L −1 CaCl 2 buffered with 0.05 mol L −1 Tris buffer (pH 7.4) and Solution 2 was 0.06 mol L −1 Na 2 HPO 4 solution buffered with 0.05 mol L −1 Tris buffer (pH 9.0).…”

Section: Methodsmentioning

confidence: 99%

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In Vitro Mineralization Study of Chitosan/Carbon Nanotubes Scaffolds: Effect of Mineralization Cycles

Horn

Martins

Plepis

2018

Macromolecular Symposia

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In this study, bioactive scaffolds of chitosan/carbon nanotubes with calcium phosphate deposits with potential use in bone tissue regeneration were developed. Alternate mineralization processes using 1, 2, or 3 cycles are performed to investigate the nucleation of phosphate salts in the scaffolds. Characterization is carried out by thermogravimetry (TG), scanning electron microscopy (SEM), energy dispersive X‐ray spectroscopy (EDX), and X‐ray diffraction (XRD). TG curves confirmed that the number of mineralization cycles influence the amount of nucleated inorganic phosphate with an increase of up to 22%. As observed by SEM, the quantity and the size of pores decreased as a function of the number of mineralization cycles. The nucleated mineral is spherical in shape and with an average size of 0.73 ± 0.10 μm. An increase in the mineralization cycles promoted an increase in the quantity of calcium phosphate and the Ca/P ratio also showed dependence with the mineralization cycles. As observed by XRD, calcium phosphate deposited in scaffolds is calcium deficient hydroxyapatite. The crystallite size is estimated using the Sherreŕs equation as being of 15 ± 1 nm.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

In Vitro Mineralization Study of Chitosan/Carbon Nanotubes Scaffolds: Effect of Mineralization Cycles

Horn

Martins

Plepis

2018

Macromolecular Symposia

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“…Sulfonic functional groups have been reported to be introduced to polymer surfaces by solution-based sulfonation reactions in work led by Leonor [45]. High molecular weight (HMW) polyethylene (HMWPE) plates were sulfonated by a heterogeneous reaction involving treatment with 50 vol % chlorosulfonic acid [46][47][48][49].…”

Section: Resultsmentioning

confidence: 99%

“…High molecular weight (HMW) polyethylene (HMWPE) plates were sulfonated by a heterogeneous reaction involving treatment with 50 vol % chlorosulfonic acid [46][47][48][49]. For the functionalization of polyethylene terephthalate (PET), Nylon-6 and the ethylene vinylalcohol (EVOH), they applied a sulfuric acid solution at concentrations from 10 to 50 vol % depending on the substrate [45,46]. They reported successful introduction of the sulfonic group based on Fourier transform infrared spectroscopy (FTIR) and no evidence of degradation of the bulk polymers was determined.…”

Section: Resultsmentioning

confidence: 99%

Effect of Diazotated Sulphonated Polystyrene Films on the Calcium Oxalate Crystallization

et al. 2017

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Pathological crystallization of calcium oxalate (CaOx) inside the urinary tract is called calculi or kidney stone (Urolithiasis). CaOx exhibits three crystalline types in nature: CaOx monohydrate COM, dihydrate COD and trihydrate COT. COD and COM are often found in urinary calculi, particularly COM. Langmuir monolayers, membrane vesicles, phospholipids' micelles, among others, have been adopted as simplified biomimetic template-models to study in vitro the urolithiasis through CaOx. The nucleation and crystal growth of COM on self-assembled lipid monolayers have revealed that the negatively charged phosphatidylserine interface is a strong promoter of COM. Herein, we describe the synthesis and physicochemical characterization of diazotated sulphonated polystyrene films (DSPFs), prepared from various aminocompounds varying their polarity degree i.e., polar, non-polar and acidic DSPF derivatives. We also used these DSPFs as polymeric templates in crystallization experiments of CaOx in vitro. Images obtained by optical microscopy and scanning electron microscopy confirmed the precipitation of COM crystals on the DSPF surface. The employment of functionalized polymeric films as templates for CaOx crystallization represents a viable approach for understanding inorganic mineralization.

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“…Acellular simulated body fluid (SBF) with ion concentrations equal to human blood plasma is widely used to study calcification of synthetic materials …”

Section: Methodsmentioning

confidence: 99%

Calcification resistance of polyisobutylene and polyisobutylene‐based materials

Kekec

Akolpoglu

Bozuyuk

et al. 2019

Polymers for Advanced Techs

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Calcification of implanted biomaterials is highly undesirable and limits clinical applicability. Experiments were carried out to assess the calcification resistance of polyisobutylene (PIB), PIB‐based polyurethane (PIB‐PU), PIB‐PU reinforced with (CH3)3N+CH2CH2CH2NH2 I−‐modified montmorillonite (PIB‐PU/nc), PIB‐based polyurethane urea (PIB‐PUU), PIB‐PU containing S atoms (PIBS‐PU), PIBS‐PU reinforced with (CH3)3N+CH2CH2CH2NH2 I−‐modified montmorillonite (PIBS‐PU/nc), and poly(isobutylene‐b‐styrene‐b‐isobutylene) (SIBS), relative to that of a clinically widely implanted polydimethylsiloxane (PDMS)–based PU, Elast‐Eon (the “control”). Samples were incubated in simulated body fluid for 28 days at 37°C, and the extent of surface calcification was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), energy‐dispersive X‐ray spectroscopy (EDX), X‐ray photoelectron spectroscopy (XPS), and Fourier‐transform‐infrared (FT‐IR) spectroscopy. Whereas the PDMS‐based PU showed extensive calcification, PIB and PIB‐PU containing 72.5% PIB, ie, a polyurethane whose surface is covered with PIB, were free of calcification. PIBS‐PU and PIB‐PUU, ie, polyurethanes that contain S or urea groups, respectively, were slightly calcified. The amine‐modified montmorillonite‐reinforcing agent reduced the extent of calcification. SIBS was found slightly calcified. Evidently, PIB and materials fully coated with PIB are calcification resistant.

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In vitro mineralization of functional polymers

Cited by 54 publications

References 104 publications

In Vitro Mineralization Study of Chitosan/Carbon Nanotubes Scaffolds: Effect of Mineralization Cycles

In Vitro Mineralization Study of Chitosan/Carbon Nanotubes Scaffolds: Effect of Mineralization Cycles

Effect of Diazotated Sulphonated Polystyrene Films on the Calcium Oxalate Crystallization

Calcification resistance of polyisobutylene and polyisobutylene‐based materials

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