In Vitro Micropropagation of Yew (Taxus Baccata) and Production of Plantlets

Abbasin, Z.; Zamani, S M; Movahedi, Sara; Khaksar, G.; Tabatabaei, Badraldin Ebrahim Sayed

doi:10.3923/biotech.2010.48.54

Cited by 24 publications

(11 citation statements)

References 16 publications

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“…Generally, half-strength of all different nutrient media improved the overall growth of roots compared to full strength salts of nutrient media for roots formation of Z. officinale. This result goes in line with those found by Abbasin et al [1] who confirmed that, half strength basal salts media were more effective than corresponding full strength basal salts media for root formation of Taxus species. Results showed significant differences at the level of 5% among the applied different treatments.…”

Section: Effect Of Different Types Of Nutrient Media On Roots Formationsupporting

confidence: 94%

In vitro propagation of ginger (Zingiber officinale Rosco)

Abbas

Taha

Aly

et al. 2011

Journal of Genetic Engineering and Biotechnology

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An efficient and promising protocol for in vitro propagation of Zingiber officinale Rosco using sprouting buds was established. Sprouting buds were sterilized and cultured onto MS medium supplemented with different growth regulators. Augmentation of MS-medium with 4.5 mg/l BAP recorded the highest percentage of shootlets multiplication. Shootlets were highly rooted on half strength of B 5 medium supplemented with 1.0 mg/l NAA. The maximum percentage of acclimatization, hardening and rhizomes production of in vitro derived plants in greenhouse was 80-100%.

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Section: Effect Of Different Types Of Nutrient Media On Roots Formationsupporting

confidence: 94%

In vitro propagation of ginger (Zingiber officinale Rosco)

Abbas

Taha

Aly

et al. 2011

Journal of Genetic Engineering and Biotechnology

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“…According to Da Silva et al (2005) 2,4-D is the main synthetic auxin used for induction of callogenesis because it has great capacity to efficiently stimulate cell division of tissues in several plants. NAA and 2, 4-D are commonly used with BA for callus induction in various plant systems (Dhar and Josh, 2005;Agrawal and Sadar, 2007;Abbasin et al, 2010). Callus formation at the cut surface of the leaves was observed in hormone free media after 58 days of culture.…”

Section: Discussionmentioning

confidence: 99%

Studies on Hollarrhena floribunda (G. Don) Durand Schinz: Germination and seedling growth, and preliminary investigations on callus induction and plant regeneration

Ayisire¹,

Ayisire²,

Akinbola³

2012

Afr. J. Agric. Res.

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“…All natural sources produce low levels of PC [9][10][11]. Although PC can be synthesized through total chemical synthesis, it is found time-consuming, expensive, and low-yielding due to the complexity of the chemical structure of PC [12,13]. Hence, in response to the increasing demand for the supplies of PC, new alternative approaches are required to be developed.…”

Section: Introductionmentioning

confidence: 99%

Identification and in-silico characterization of taxadien-5α-ol-O-acetyltransferase (TDAT) gene in Corylus avellana L.

et al. 2021

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Paclitaxel® (PC) is one of the most effective and profitable anti-cancer drugs. The most promising sources of this compound are natural materials such as tissue cultures of Taxus species and, more recently, hazelnut (Corylus avellana L.). A large part of the PC biosynthetic pathway in the yew tree and a few steps in the hazelnut have been identified. Since understanding the biosynthetic pathway of plant-based medicinal metabolites is an effective step toward their development and engineering, this paper aimed to identify taxadiene-5α-ol-O-acetyltransferase (TDAT) in hazelnut. TDAT is one of the key genes involved in the third step of the PC biosynthetic pathway. In this study, the TDAT gene was isolated using the nested-PCR method and then characterized. The cotyledon-derived cell mass induced with 150 μM of methyl jasmonate (MeJA) was utilized to isolate RNA and synthesize the first-strand cDNA. The full-length cDNA of TDAT is 1423 bp long and contains a 1302 bp ORF encoding 433 amino acids. The phylogenetic analysis of this gene revealed high homology with its ortholog genes in Quercus suber and Juglans regia. Bioinformatics analyses were used to predict the secondary and tertiary structures of the protein. Due to the lack of signal peptide, protein structure prediction suggested that this protein may operate at the cytoplasm. The homologous superfamily of the T5AT protein, encoded by TDAT, has two domains. The highest and lowest hydrophobicity of amino acids were found in proline 142 and lysine 56, respectively. T5AT protein fragment had 24 hydrophobic regions. The tertiary structure of this protein was designed using Modeler software (V.9.20), and its structure was verified based on the results of the Verify3D (89.46%) and ERRAT (90.3061) programs. The T5AT enzyme belongs to the superfamily of the transferase, and the amino acids histidine 164, cysteine 165, leucine 166, histidine 167, and Aspartic acid 168 resided at its active site. More characteristics of TDAT, which would aid PC engineering programs and maximize its production in hazelnut, were discussed.

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In Vitro Micropropagation of Yew (Taxus Baccata) and Production of Plantlets

Cited by 24 publications

References 16 publications

In vitro propagation of ginger (Zingiber officinale Rosco)

In vitro propagation of ginger (Zingiber officinale Rosco)

Studies on Hollarrhena floribunda (G. Don) Durand Schinz: Germination and seedling growth, and preliminary investigations on callus induction and plant regeneration

Identification and in-silico characterization of taxadien-5α-ol-O-acetyltransferase (TDAT) gene in Corylus avellana L.

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