In vitro micropropagation of Dracaena sanderiana Sander ex Mast: An important indoor ornamental plant

Abstract

A protocol has been developed for in vitro plant regeneration from a nodal explant of Dracaena sanderiana Sander ex Mast. Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 μM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 μM chlorophenoxy acetic acid (CPA). The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 μM N(6)-benzylaminopurine (BA). Rooting was high on MS solid compared to liquid medium when added with 7.38 μM indole-3-butyric acid (IBA). Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1). In vitro and ex vitro raised plantlets were used for acclimatization. More than 90% of the plantlets was successfully acclimatized and established in plastic pots. Ex vitro transferred plantlets were normal without any phenotypic aberrations.

Callus induction, shoot proliferation and root regeneration of potato (Solanum tuberosumL.) stem node and leaf explants under long-day conditions

“…All plants transferred to the ex vitro conditions showed a high homogeneity without an obvious morphological avoidance of somaclonal variation. [28] Conclusions A well-developed callus from different explants and PGRs may be used for the development of new potato lines and overall improvement of potato genotypes through biotechnological approach. The regeneration of shoots is also considered as a limiting step for the successful use of modern techniques in genetic improvement of potato crop.…”

Towards the micropropagation of Euphorbia cyathophora Murray: a wild plant species with medicinal and ornamental potential

“…It is important to point out that an inversely proportional effect was observed between these variables in which the higher root number, the lower root length. Commonly, IBA is used to generate roots in explants of several ornamental plants such as Ficus carica, F. benjamina, Gerbera jamesonii, Yucca aloifolia, Dracaena sanderiana, Jatropha curcas, Glochidion multiloculaire, Euphorbia antisyphilitica, and E. pugniformis (ROUT et al, 2006;ASLAM et al, 2013). Nevertheless, there are reports in where BAP, alone or combined with NAA, efficiently promotes the root generation in G. jamesonii, Manihot esculenta, and Dieffenbachia amoena (ELSHEIKH et al, 2013).…”

Multiplicación in vitro y embriogénesis somática de Perezia pinnatifida (Asteraceae) planta medicinal andina

“…La adición de BAP incrementa la formación de brotes en diversas plantas medicinales (Aslam et al 2013, Shekhawat et al 2015, Elamvaluthi et al 2016; probablemente porque las citoquininas activan a las yemas axilares de los explantos desencadenando la proliferación de brotes (Aremu et al 2016). En concordancia, la adición de BAP en el cultivo in vitro de P. pinnatifida fue esencial para la multiplicación de brotes, al igual que en Chlorophytum borivilianum (Shekhawat et al 2015).…”