In Vitro Micro Tuber Formation in Potato (Solanum tuberosum L.): is there any Relation between Methyl Jasmonate, Sugars, and Explants?

Abstract

Studies on potato (Solanum tuberosum L.) plants have been carried out to plant tissue culture laboratories because of the disease and virulent free seed tubers in field conditions. Since generative production in commercial potato production is not economically massive, production is made more economically with tubers. Jasmonic acid (JA) and its related compounds are newly recognized plant growth regulators and they are known to have effects on many physiological processes. In this study, it was aimed to determine the effects of methyl jasmonate and sucrose on micro tuber formation in potato micropropagation. To serve the purpose the relationships between twelve media combinations (including control medium), varying concentrations of sucrose (3, 6 and 9 %) either alone or in combination with 1.0 ppm or 2.0 ppm methyl jasmonate were investigated. Three different plant parts such as node, shoot eye and primordial tissue wereused as explant sources. Nitsch&Nitsch was used as the basic nutrient medium and plant growth regulators that used in media were constant at 2.5 mg/L BAP + 0.5 mg/L NAA except for control medium. Obtained micro tubers, plantlets and callus formations were recorded and analyzed. Results revealed that, when methyl jasmonate used at 1.0 ppm concentration in media, it had a positive effect on micro tuber formation and can be recommended to use commercially in mass production.

Effect of Thidiazuron and 6-Benzylaminopurine on in vitro microtuberization in potato cv. Spunta.

“…In addition to the high level of sucrose which represents the milestone in the culture medium for microtuber induction in potato, the plant growth regulator in particular the cytokinins proved to be essential for enhancing microtuberization in different potatoes genotypes. Many studies were used BAP for increasing the production of microtubers in potato (Aboshama et al 2006;Momena et al 2014;Dhaka and Nailwal, 2015;Mohapatra et al 2016;Emaraa et al 2017;Khalil et al 2017;Moreno et al 2017;Salem And Hassanein, 2017;Ali et al 2018;Vural et al 2018;Borna et al 2019;Hossain et al 2019;Naqvi et al 2019;Khorsandi et al 2020;Refaie et al 2020;Sembiring et al 2020;Sota et al 2020;Andriani et al 2021).…”

Effects of hypergravity in a centrifuge and simulated microgravity on a horizontal clinostat on tuber formation in single-nodal stem segments from potato (<i>Solanum tuberosum </i>L.) sprouts

“…Propagation of potato plants can be achieved in several ways, including tuber sprouts, tuber cuttings, and true seeds. It has also been reported that in vitro systems based on a single-nodal stem explant result in the differentiation of stolon into a tuber instead of a leafy shoot when placed on a tuber-inducing medium, which is characterized by a high sucrose content and by supplementation with tuber-inducing plant growth substances, and incubated in the dark (Xu et al, 1998;Fernie and Willmitzer, 2001;Vural et al, 2018). Here, we report the effects of hypergravity in a centrifuge on potato tuber formation using single-nodal stem segments from the aspects of soluble sugar and starch content, as well as the effect of hypergravity on stem growth.…”

Effect of Potassium Dihydrogen Orthophosphate and Sucrose Concentration on Potato Growth and Development by In Vitro

“…Micropropagation is the alternative to the conventional propagation of potatoes. This technique is used to obtain disease and virus-free plant materials [10]. In vitro micropropagation in potatoes is generally used to bulk up new cultivars and breeding lines, for germplasm storage, transport, and production micro tuber which are easy to store, transfer, and distribute [11].…”