In vitro meristem culture of M.4 apple (Malus pumila Mill.). I. Optimal nutrient medium

Ochatt, Sergio; Caso, O. H.

doi:10.1007/bf00033551

Cited by 8 publications

(4 citation statements)

References 7 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…0.5-2 cm long shoots. These results contrast to those reported by Ochatt and Caso [29] for meristems of M4 apple, because in 'Oregon spur-II', number of shoots per explant were increased when both BA and GA3 concentrations in multiplication medium decreased. Moreover, greatest size and internode enlargement made it possible to separate the shoots.…”

Section: In Vitro Shoot Multiplicationcontrasting

confidence: 99%

Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’

Vivek

Modgil

2018

VirusDis.

View full text Add to dashboard Cite

In the present study, an attempt was made to eliminate apple chlorotic leaf spot virus, apple mosaic virus, apple stem grooving virus and apple stem pitting virus from apple cultivar 'Oregon Spur-II'. Thermotherapy was carried out at 37-40 °C for 4 weeks followed by culturing of meristems of different sizes. During establishment of explants, highest survival percentage (62.35%) and proliferation (30.68%) was recorded during summer season. However, size of meristems and position of buds from where meristems were excised also influenced their survival. The meristems of size 0.6-0.7 mm were found to be the most appropriate for maximum establishment. Meristems excised from buds positioned on distil portions of actively growing shoots showed better results. MS medium supplemented with BA (1.0 mg/l), IBA (0.05 mg/l) and GA (0.1 mg/l) resulted in 56.62% establishment of explants, while maximum number of meristems proliferated with low BA (0.5 mg/l), IBA (0.08 mg/l) and same GA concentration. Two to fourfold multiplication was observed. Virus indexing of shoots raised from different sizes of meristems was carried out and found that 0.3-0.6 mm size was able to eliminate ACLSV, ApMV, ASGV and ASPV. However, some of 0.5-0.6 mm sized shoots were found infected with ACLSV. Larger meristems could not completely eliminate the viruses under study.

show abstract

Section: In Vitro Shoot Multiplicationcontrasting

confidence: 99%

Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’

Vivek

Modgil

2018

VirusDis.

View full text Add to dashboard Cite

show abstract

“…Solanum tuberosum L. cv. Kennebec plants regenerated from a saltsusceptible callus line (clone S) or from a line of the same genotype regenerated after recurrent selection for tolerance to 150 mM NaCl (clone 150) (Ochatt et al 1999) were used.…”

Section: Plant Culture and Salt Treatmentsmentioning

confidence: 99%

“…Sodium chloride tolerance was accompanied by a significant increase in antioxidant activity in a cotton cell line grown under NaCl stress (Gosset et al 1996). Ochatt et al (1999) have recently reported the in vitro production of a stable salt-tolerant cell line of potato (Solanum tuberosum L. cv. Kennebec) by direct recurrent selection.…”

Section: Introductionmentioning

confidence: 99%

Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum

Benavides¹,

Marconi²,

Gallego³

et al. 2000

Functional Plant Biol.

View full text Add to dashboard Cite

A relationship between the antioxidant defence system and salt tolerance in two clones of potato (Solanum tuberosum L.) differing in salt sensitivity was studied. The antioxidant defence system of the sensitive clone responded differently to 100 and 150 mM NaCl. At 100 mM NaCl, growth, dehydroascorbate reductase and catalase activities remained unaltered, but chlorophyll and reduced glutathione content decreased (23% and 35%, respectively), while ascorbate content and superoxide dismutase activity were increased 34% and 63%, with respect to the control (0 mM NaCl). The superoxide dismutase increment was higher under 150 mM NaCl treatment, while a general decrease (except for dehydroascorbate reductase and catalase activities) in all the antioxidant parameters studied was observed in the sensitive clone. Reduced glutathione and ascorbate, the main antioxidant soluble defences, and all antioxidant enzymes (except catalase) were significantly elevated in the tolerant clone compared to the sensitive one when both were grown in the absence of NaCl. Under 100 and 150 mM NaCl treatments, no changes in the antioxidant stress parameters were detected in the tolerant clone. These results suggest a relationship between salt tolerance and the antioxidant defence system in the two clones.

show abstract

“…Other factors shown to affect shoot proliferation in apple include phloroglucinol, light levels, explant type, agar source, sugar source, pH, and auxin concentration [ 13,25,39] . Shoots from apple seedlings and rootstocks proliferate and root in vitro relatively easily [7,12,23,32,37] . Micropropagation of rootstocks results in rejuvenation of clones making them easier to propagate by conventional techniques [13,29,30] .…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of thirteen Malus cultivars and rootstocks, and effect of antibiotics on proliferation

Yepes

Aldwinckle

1994

Plant Growth Regul

View full text Add to dashboard Cite

Several factors that affect in vitro establishment, proliferation, and rooting of thirteen Malus cultivars and rootstocks were studied . Apple shoot tips (1 .5 ± 0.5 cm in length) were established using ascorbic and citric acids as antioxidants . Four proliferation media containing 1 .0 mg 1 -1 BA and different concentrations of IBA and GA3 were tested. Proliferation rates varied depending on the genotype and medium used . The highest proliferation rate was obtained for a rootstock that produced 11 .6 ± 2 .5 shoots (1 .5 ± 0 .8 cm in length) per tube per month . Rooting was induced with IBA for all the genotypes tested . The optimal IBA concentration was cultivar dependent (between 0 .1 and 1 .0 mg 1-1 IBA), and lower concentrations were necessary to induce rooting in liquid rather than in solid medium .The effects on shoot-tip proliferation of cefotaxime, carbenicillin and kanamycin, three antibiotics commonly used for transformation studies, were also evaluated . Cefotaxime at 200 mg 1 -1 stimulated shoot growth and development, but at 500 mg 1 -1 caused abnormal shoot morphology. Carbenicillin at 500 mg 1 -1 , alone or in combination with cefotaxime at 200 mg 1 -1 , inhibited proliferation and caused excessive enlargement of the basal leaves, inducing callus formation and release of phenolic compounds in the medium . Kanamycin at 50 mg 1 -1 was phytotoxic and caused shoot chlorosis and necrosis . Consideration of the toxicity of these antibiotics is critical when designing transformation schemes for selection and recovery of transgenic apple plants .Abbreviations: BA = benzyladenine ; cef = cefotaxime ; crb = carbenicillin ; GA3 = gibberellic acid ; IBA = indole-3-butyric acid ; Kan = kanamycin ; ms = Murashige and Skoog [19] macro-and micro-nutrients ; NAA = naphthalene-acetic acid . 55

show abstract

In vitro meristem culture of M.4 apple (Malus pumila Mill.). I. Optimal nutrient medium

Cited by 8 publications

References 7 publications

Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’

Elimination of viruses through thermotherapy and meristem culture in apple cultivar ‘Oregon Spur-II’

Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum

Micropropagation of thirteen Malus cultivars and rootstocks, and effect of antibiotics on proliferation

Contact Info

Product

Resources

About