In Vitro Membrane Platform for the Visualization of Water Impermeability across the Liquid-Ordered Phase under Hypertonic Conditions

Baek, Ji Min; Jung, Woo Hyuk; Yu, Eui‐Sang; Ahn, Dong June; Ryu, Yong‐Sang

doi:10.1021/jacs.2c06626

Cited by 4 publications

(4 citation statements)

References 54 publications

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“…These characteristics reduce water permeability. In line with these findings, molecular dynamics (MD) simulations indicated that the presence of cholesterol in the 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) bilayer[6], 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) bilayer[7], phosphatidylglycerol bilayer[8] and dioleylphosphatidylcholine (DOPC)/ sphingomyelin bilayer[9] significantly reduced water permeability, demonstrating that reduced water permeability was caused by the increased organization of lipids in the presence of cholesterol.…”

Section: Introductionmentioning

confidence: 97%

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The translocation mechanism of calcitriol throughHelicobacter pylorilipid membrane and influence on water permeability

Cao

Zhao

Chen

et al. 2023

Preprint

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Helicobacter pylori exhibits a unique membrane lipid composition, including dimyristoyl phosphatidylethanolamine (DMPE) and cholesterol, unlike other Gram-negative bacteria. Calcitriol has antimicrobial activity against H. pylori, but cholesterol enhances antibiotics resistance in H. pylori. This study explored the changes in membrane structure and the molecular mechanisms of cholesterol/calcitriol translocation using well-tempered metadynamics (WT-MetaD) simulations and microsecond conventional molecular dynamics simulations. Our results showed that the average area per lipid and sterol tilt angles were slightly lower, while DP-P, DCG-CG, DAC-AC, and SCDwere higher in cholesterol membrane systems than in calcitriol membrane systems. Cholesterol membrane systems were more ordered than calcitriol-containing membranes. Calcitriol facilitated water transport across the membrane, while cholesterol had the opposite effect. The differing effects might result from the tail 25-hydroxyl group and a wider range of orientations of calcitriol in the DMPE/ dimyristoyl phosphatidylglycerol (DMPG) (3:1) membrane. Calcitriol moves across the bilayer center without changing its orientation along the membrane Z-axis, becomes parallel to the membrane surface at the membrane-water interface, and then rotates approximately 90º in this interface. The translocation mechanism of calcitriol is quite different from the flip-flop of cholesterol. Moreover, calcitriol crossed from one layer to another more easily than cholesterol, causing successive perturbations to the hydrophobic core and increasing water permeation. These results improve our understanding of the relationship between cholesterol/ calcitriol concentrations and the lipid bilayer structure and the role of lipid composition in water permeation.

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Section: Introductionmentioning

confidence: 97%

“…phosphatidylglycerol bilayer [8] and dioleylphosphatidylcholine (DOPC)/ sphingomyelin bilayer [9] significantly reduced water permeability, demonstrating that reduced water permeability was caused by the increased organization of lipids in the presence of cholesterol.…”

Section: Introductionmentioning

confidence: 99%

The translocation mechanism of calcitriol throughHelicobacter pylorilipid membrane and influence on water permeability

Cao

Zhao

Chen

et al. 2023

Preprint

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“…In mixtures containing cholesterol that exhibit phase separation into liquid ordered (L o ) and liquid disordered (L d ) phases, the L o phase has lower water permeability. , However, the difference in water permeability between L o and L d is significantly lower than the difference between the permeability of gel and fluid phases of single-component lipid membranes. This difference is attributed to a permeation pathway that includes diffusion into the L d regions at the L o /L d boundary with subsequent diffusion along the membrane midplane …”

Section: Introductionmentioning

confidence: 99%

The Chemical Reactivity of Membrane Lipids

Duché,

Sanderson

2024

Chem. Rev.

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It is well-known that aqueous dispersions of phospholipids spontaneously assemble into bilayer structures. These structures have numerous applications across chemistry and materials science and form the fundamental structural unit of the biological membrane. The particular environment of the lipid bilayer, with a water-poor low dielectric core surrounded by a more polar and better hydrated interfacial region, gives the membrane particular biophysical and physicochemical properties and presents a unique environment for chemical reactions to occur. Many different types of molecule spanning a range of sizes, from dissolved gases through small organics to proteins, are able to interact with membranes and promote chemical changes to lipids that subsequently affect the physicochemical properties of the bilayer. This Review describes the chemical reactivity exhibited by lipids in their membrane form, with an emphasis on conditions where the lipids are well hydrated in the form of bilayers. Key topics include the following: lytic reactions of glyceryl esters, including hydrolysis, aminolysis, and transesterification; oxidation reactions of alkenes in unsaturated fatty acids and sterols, including autoxidation and oxidation by singlet oxygen; reactivity of headgroups, particularly with reactive carbonyl species; and E/Z isomerization of alkenes. The consequences of reactivity for biological activity and biophysical properties are also discussed. CONTENTS

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“…Molecular dynamics simulation (MD) is a widely used method to get the molecular level understanding of the cation-membrane interaction at physiological conditions or osmotic pressures. − As metal ions mainly interact with the outer membrane of RBCs, we constructed model membranes by 1,2-dioleoylsn-glycerol-3-phosphocholine (DOPC), which is one of the most abundant PC in the outer leaflet of the cell membrane of RBCs. ,− Using the CHARMM-GUI program, we designed four simulation systems, each consisting of 128 DOPC molecules (and about 6000 water molecules) with different metal ions: K(I), Ca(II), Zn(II), or no metal ions (DOPC with water only). Figure c shows a typical snapshot of the system with Zn(II), and the others are shown in Figure S14.…”

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confidence: 99%

Zinc Cations Uniquely Stabilize Cell Membrane for Cell Cryopreservation

Zheng,

Zhang,

Cao

et al. 2023

Nano Lett.

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We report, for the first time, merely using a small amount of (0.039% w/w) Zn(II) instead of very high concentration (25%–50% w/w) of conventional cryoprotective agents (CPAs), i.e., glycerol, during the cryopreservation of red blood cells (RBCs) can lead to a comparable post-thaw recovery rate of ∼95% while avoiding the tedious gradient washout process for the removal of CPA afterward. The result is remarkable, since Zn(II) does not have the ice-controlling ability reported to be critical for CPA. It benefits from its moderate interaction with lipid molecules, facilitating the formation of small and dynamic lipid clusters. Consequently, the membrane fluidity is maintained, and the cells are resilient to osmotic and mechanical stresses during cryopreservation. This study first reports the ion-specific effect on stabilizing the cell membrane; meanwhile, reversibly tuning the structure of biological samples against injuries during the cooling and rewarming provides a new strategy for cryopreservation.

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In Vitro Membrane Platform for the Visualization of Water Impermeability across the Liquid-Ordered Phase under Hypertonic Conditions

Cited by 4 publications

References 54 publications

The translocation mechanism of calcitriol throughHelicobacter pylorilipid membrane and influence on water permeability

The translocation mechanism of calcitriol throughHelicobacter pylorilipid membrane and influence on water permeability

The Chemical Reactivity of Membrane Lipids

Zinc Cations Uniquely Stabilize Cell Membrane for Cell Cryopreservation

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