In Vitro Measurements of Extracellular l-Glutamate Level in Region CA3 of Mouse Hippocampal Slices under Chemical Stimulation

Chiba, Hiromi; Deguchi, Yukari; Kanazawa, Ena; Kawai, Jun; Nozawa, Keiichiro; Shoji, Atsushi; Sugawara, Masao

doi:10.2116/analsci.26.1103

Cited by 5 publications

(2 citation statements)

References 26 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such a differential response is buttressed by a previous observation that in the presence of TBOA, tetraethylammonium chloride elicits a several fold greater glutamate release in CA3 than in CA1 or DG (Chiba et al . ). However, specific mechanisms may vary between these two paradigms.…”

Section: Discussionmentioning

confidence: 97%

Peripherally restricted viral challenge elevates extracellular glutamate and enhances synaptic transmission in the hippocampus

Hunsberger

Wang

Petrisko

et al. 2016

Journal of Neurochemistry

View full text Add to dashboard Cite

Peripheral infections increase the propensity and severity of seizures in susceptible populations. We have previously shown that intraperitoneal injection of a viral mimic, polyinosinicpolycytidylic acid (PIC), elicits hypersusceptibility of mice to kainic acid (KA)-induced seizures. This study was undertaken to determine whether this seizure hypersusceptibility entails alterations in glutamate signaling. Female C57BL/6 mice were intraperitoneally injected with PIC, and after 24 h, glutamate homeostasis in the hippocampus was monitored using the enzyme-based microelectrode arrays. PIC challenge robustly increased the level of resting extracellular glutamate. While presynaptic potassium-evoked glutamate release was not affected, glutamate uptake was profoundly impaired and non-vesicular glutamate release was augmented, indicating functional alterations of astrocytes. Electrophysiological examination of hippocampal slices from PIC-challenged mice revealed a several fold increase in the basal synaptic transmission as compared to control slices. PIC challenge also increased the probability of pre-synaptic glutamate release as seen from a reduction of paired-pulse facilitation and synaptic plasticity as seen from an enhancement of long-term potentiation. Altogether, our results implicate a dysregulation of astrocytic glutamate metabolism and an alteration of excitatory synaptic transmission as the underlying mechanism for the development of hippocampal hyperexcitability, and consequently seizure hypersusceptibility following peripheral PIC challenge.

show abstract

Section: Discussionmentioning

confidence: 97%

Peripherally restricted viral challenge elevates extracellular glutamate and enhances synaptic transmission in the hippocampus

Hunsberger

Wang

Petrisko

et al. 2016

Journal of Neurochemistry

View full text Add to dashboard Cite

show abstract

“…Combination of electrophysiological and sensor techniques provides a useful, often essential information on the targeted neuronal events. Recordings of L-glutamate currents at electrochemical sensors have been reported by using different type of electrodes such as an excised patch technique [28,23,22,20,11,37], a whole-cell technique using astrocyte transporter currents [21,12], and an in vivo method using a dialysis electrode [13]. Enzyme-based sensors have also been extensively used for in vivo and in vitro monitoring of extracellular L-glutamate [7,26,35,34,39,38,32,17,33].…”

Section: Introductionmentioning

confidence: 99%

Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Ikegami

Hozumi

Shoji

et al. 2014

Sensing and Bio-Sensing Research

Self Cite

View full text Add to dashboard Cite

Keywords:L-glutamate sensor High-frequency stimulation Field excitatory postsynaptic potential Mouse hippocampal slice Long-term potentiation a b s t r a c t Real-time monitoring of L-glutamate released by high-frequency stimulation in region CA1 of mouse hippocampal slices was performed with a glass capillary-based sensor, in combination with the recoding of excitatory postsynaptic potentials (fEPSPs). A method for extracting L-glutamate currents from the recorded ones was described and applied for determining the level of extracellular L-glutamate released by 100 Hz stimulation. Recording of an L-glutamate current with a current sampling interval of 1 Hz was found to be useful for acquiring a Faradaic current that reflects L-glutamate level released by the highfrequency stimulation of 7 trains, each 20 stimuli at 100 Hz and inter-train interval of 3 s. The L-glutamate level was obtained as 15 ± 6 lM (n = 8) for the persistent enhancement of fEPSPs, i.e., the induction of longterm potentiation (LTP), and 3 ± 1 lM (n = 5) for the case of no LTP induction. Based on these observations, the level of the extracellular L-glutamate was shown to play a crucial role in the induction of LTP.

show abstract

Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

Hozumi

Ikezawa

Shoji

et al. 2011

Biosensors and Bioelectronics

View full text Add to dashboard Cite

In Vitro Measurements of Extracellular l-Glutamate Level in Region CA3 of Mouse Hippocampal Slices under Chemical Stimulation

Cited by 5 publications

References 26 publications

Peripherally restricted viral challenge elevates extracellular glutamate and enhances synaptic transmission in the hippocampus

Peripherally restricted viral challenge elevates extracellular glutamate and enhances synaptic transmission in the hippocampus

Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor

Simultaneous monitoring of excitatory postsynaptic potentials and extracellular l-glutamate in mouse hippocampal slices

Contact Info

Product

Resources

About