In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors

Marques, Mariana Groke; Nicacio, Alessandra Corallo; Oliveira, Viviane de Cássia; Nascimento, A. B.; Caetano, H.V.A.; Mendes, Clayton Quirino; Mello, Marco Roberto Bourg de; Milazzotto, Marcella Pécora; Assumpção, Mayra Elena Ortiz D’Ávila; Visintin, José Antônio

doi:10.1016/j.anireprosci.2006.02.013

Cited by 33 publications

(40 citation statements)

References 14 publications

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“…Similar results were obtained by Marques et al (2007) in medium with EGF (65.00 %) in pig oocytes. Abeydeera et al (2000) and Kishida et al (2004) reported higher maturation rates of 84.8 and 77.4 per cent respectively in medium with EGF in pig oocytes.…”

Section: Resultssupporting

confidence: 78%

Effect of growth factor on in-vitro maturation of porcine oocyte

Mahanta

Bhuyan

Kumar

et al. 2018

IJAR

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The present study was aimed to evaluate the beneficial effect of different growth factors on in-vitro maturation of porcine oocytes. Ovaries were collected from a local abattoir immediately after slaughter of the animals and transported to the laboratory. A total of 618 type A and type B oocytes were cultured in TCM-199 containing additives with PMSG and hCG for the first 22 hrs and without hormones for subsequent 22 hrs of incubation at 39 o C under 5 per cent CO 2 level and 90-95 per cent humidity. The effects of supplementation of different growth factors viz., EGF, IGF-I and EGF + IGF-I in the medium were studied. The rate of oocytes with cumulus cells expansion was significantly higher (P<0.01) when growth factors were added as compared to control but it did not differ significantly between growth factors. The rate of nuclear maturation of oocytes was significantly higher (P<0.01) as compare to control for EGF and EGF + IGF-I but not for IGF-I. There was no significant difference in the rate of oocytes with nuclear maturation between the growth factors studied. It can be concluded from the present study that addition of EGF, IGF-I or EGF + IGF and additives along with hormones (PMSG and hCG for first 20-22 hrs) in TCM-199 Medium gives optimum in-vitro maturation rates in porcine oocytes.

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Section: Resultssupporting

confidence: 78%

Effect of growth factor on in-vitro maturation of porcine oocyte

Mahanta

Bhuyan

Kumar

et al. 2018

IJAR

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“…Fetal calf serum (FCS), fetal bovine serum (FBS) and bovine serum albumin (BSA) are the most common protein supplements of IVM media, however the controversially reports were published about their usage in follicular maturation media in different species [28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media

Abedelahi

Salehnia

Allameh

2008

J Assist Reprod Genet

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“…Funahashi and Day (1993) demonstrated that 20 h of maturation with no hormone supplementation would be necessary to improve cytoplasmic maturation. Based on the regulated sequence of hormonal changes during in vivo maturation, some reports established a two-step IVM system consisting of 22-h incubation with hCG and eCG followed by 22-h incubation with hormone-free media (Marques et al, 2007;Gil et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Progesterone improves porcine in vitro fertilisation system

Malo

Gil

Cano

et al. 2014

Acta Veterinaria Hungarica

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In an effort to improve the quality of in vitro produced porcine embryos, the effect of progestagens -progesterone analogues -on the in vitro developmental competence of porcine oocytes was studied. A total of 1421 in vitro matured oocytes, from 4 replicates, were inseminated with frozen-thawed spermatozoa. Progestagens were added to late maturation and embryo cultures (10 IU/ml). Fertilisation success (pre-maturation, penetration, monospermy and efficiency) and nuclear maturation were evaluated. There were no differences among prematuration rates between groups (P = 0.221). Penetration rates were higher (P < 0.001) in the presence of progestagens (75.0%) as compared to the control (51.7%). However, no differences were observed in monospermy percentages (P = 0.246). The results indicated that supplementation with progestagens increased the efficiency of the in vitro fertilisation system (P < 0.001). An additional beneficial effect was observed in nuclear maturation with progestagens (P = 0.035). In summary, progestagen supplementation is an important factor to improve the in vitro fertilisation procedure. Key words: In vitro fertilisation, oocyte maturation, progesterone, porcineThe overall efficiency of in vitro porcine embryo production and the quality of embryos are still low when compared with in vivo results (Funahashi, 2003). One of the major problems includes improper in vitro maturation (IVM) of oocytes, in both the nuclear and cytoplasmic compartments (Niwa, 1994;Marchal et al., 2001). While a large percentage of oocytes reached metaphase II after maturation, inadequate cytoplasmic and molecular maturation occurred (Sirard et al., 2006), leading to a lack of subsequent embryo development success. There is a lack of information on ovarian factors that may be important for the maturation and subsequent fertilisation of oocytes.The production of sex steroids by follicular cells is proposed to be influenced by the maturity of oocytes. Oestradiol, progesterone and testosterone are the main steroid hormones that play an essential role during the follicular and

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In vitro maturation of pig oocytes with different media, hormone and meiosis inhibitors

Cited by 33 publications

References 14 publications

Effect of growth factor on in-vitro maturation of porcine oocyte

Effect of growth factor on in-vitro maturation of porcine oocyte

The effects of different concentrations of sodium selenite on the in vitro maturation of preantral follicles in serum-free and serum supplemented media

Progesterone improves porcine in vitro fertilisation system

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