In Vitro Maturation and Its Role in Clinical Assisted Reproductive Technology

Sauerbrun‐Cutler, May‐Tal; Vega, Mario; Keltz, Martin D.; McGovern, Peter G.

doi:10.1097/ogx.0000000000000150

Cited by 56 publications

(35 citation statements)

References 103 publications

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“…The protocols for examination of spindles and DSBs were essentially the same as previous studies 42–45 . Briefly, for spindle and chromosome examination, oocytes were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Protective effect of antioxidants on the pre-maturation aging of mouse oocytes

Liang

Xian

et al. 2017

Sci Rep

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Pre-maturation aging of immature oocytes may adversely affect the fate of an oocyte. Oxidative stress is one of the most detrimental factors affecting oocyte developmental competence and maturation during aging. In this study, experiments were designed to examine whether supplementation of antioxidants in a culture medium could protect immature mouse oocytes from damages caused by oxidative stress. Mouse oocytes at germinal vesicle stage were prevented from meiosis resumption and cultured in a medium with or without antioxidants for 12–36 h to allow oocytes to undergo aging. After aging, oocytes were cultured for maturation. Nuclear maturation, mitochondria activity, spindle morphology and DNA integrity were examined after maturation. It was found that antioxidants had protective effects on the oocytes in terms of nuclear maturation, functional mitochondria, spindle morphology and DNA integrity. As aging time was prolonged from 12 to 36 h, the protective effect of antioxidants became more obvious. However, as compared with oocytes without aging, it was found that aging significantly inhibited nuclear maturation, impaired mitochondria function, and damaged the spindle and DNA. These results indicate that pre-maturation aging is detrimental to oocytes’ competence to undergo maturation and other cellular activities, and antioxidants can protect oocytes from damages caused by aging.

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Section: Methodsmentioning

confidence: 99%

Protective effect of antioxidants on the pre-maturation aging of mouse oocytes

Liang

Xian

et al. 2017

Sci Rep

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“…Indeed, the demand for this type of selection method has increased because IVF clinics and animal breeding facilities more and more frequently use oocytes isolated from ovaries (i.e. in ProI) and matured in vitro 24–26 . It is possible that oocytes with a different chromatin distribution, and, in consequence, a different transcriptional status, could be distinguished by a simple measurement of the mean pixel intensity inside the nucleus.…”

Section: Resultsmentioning

confidence: 99%

Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos

Karnowski

Ajduk

Wieloch

et al. 2017

Sci Rep

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Imaging of living cells based on traditional fluorescence and confocal laser scanning microscopy has delivered an enormous amount of information critical for understanding biological processes in single cells. However, the requirement for a high numerical aperture and fluorescent markers still limits researchers’ ability to visualize the cellular architecture without causing short- and long-term photodamage. Optical coherence microscopy (OCM) is a promising alternative that circumvents the technical limitations of fluorescence imaging techniques and provides unique access to fundamental aspects of early embryonic development, without the requirement for sample pre-processing or labeling. In the present paper, we utilized the internal motion of cytoplasm, as well as custom scanning and signal processing protocols, to effectively reduce the speckle noise typical for standard OCM and enable high-resolution intracellular time-lapse imaging. To test our imaging system we used mouse and pig oocytes and embryos and visualized them through fertilization and the first embryonic division, as well as at selected stages of oogenesis and preimplantation development. Because all morphological and morphokinetic properties recorded by OCM are believed to be biomarkers of oocyte/embryo quality, OCM may represent a new chapter in imaging-based preimplantation embryo diagnostics.

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“…This is among the many reasons why IVM won popularity as an alternative in the 1990s. According to a worldwide review analysis, more than 5000 babies have been born using IVM (Sauerbrun-Cutler et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Safe and successful use of oocyte in-vitro maturation in two infertile women with multiple sclerosis

Gülekli

Sezer

Gungor

et al. 2020

Reproductive BioMedicine Online

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There is evidence to support an impact of ovarian stimulation with gonadotrophin-releasing hormone analogues on the progression or recurrence of multiple sclerosis. In addition, there is no universally acknowledged approach toward ovarian stimulation in patients with multiple sclerosis. This report describes two patients at a large tertiary university hospital who underwent an in-vitro maturation protocol in order to avoid a risk of exacerbating their multiple sclerosis by ovarian stimulation. Both patients were referred to the infertility clinic because of the concern of exacerbation of multiple sclerosis during or after ovarian stimulation treatment. The patients underwent the in-vitro maturation protocol to avoid ovarian stimulating agents. Both patients gave birth to healthy babies at term. They did not suffer any relapses of multiple sclerosis during their treatment or during pregnancy. Exacerbation of disease related to ovarian stimulation encourages the search for a safer approach to these patients. To the authors' knowledge, these are the first babies described in the literature who were born after in-vitro maturation to mothers suffering from multiple sclerosis. In-vitro maturation can thus be recommended as an alternative in suitable women with multiple sclerosis.

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In Vitro Maturation and Its Role in Clinical Assisted Reproductive Technology

Abstract: Although the pregnancy rate is lower than conventional IVF, IVM is a safer and simpler alternative to conventional IVF. Future research needs to focus on improving implantation and live birth rates before universal implementation.

Cited by 56 publications

References 103 publications

Protective effect of antioxidants on the pre-maturation aging of mouse oocytes

Protective effect of antioxidants on the pre-maturation aging of mouse oocytes

Optical coherence microscopy as a novel, non-invasive method for the 4D live imaging of early mammalian embryos

Safe and successful use of oocyte in-vitro maturation in two infertile women with multiple sclerosis

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