In Vitro Maturation and In Vitro Fertilization of Human Oocytes

Wang, Weihua; Gill, Jimmy

doi:10.1385/1-59259-744-0:235

Cited by 4 publications

(8 citation statements)

References 28 publications

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“…It is also unlikely that current commercial media preparations provide sufficient levels of L-carnitine for b-oxidation. Because of the poorer developmental potential of oocytes matured in vitro compared to their counterparts matured in vivo [54], further optimization of culture medium should take fatty acid metabolic activity into consideration. We have now shown that lipid metabolism is hormonally regulated and increases in the COC during oocyte maturation in vivo.…”

Section: Discussionmentioning

confidence: 99%

Beta-Oxidation Is Essential for Mouse Oocyte Developmental Competence and Early Embryo Development1

Dunning

Cashman

Russell

et al. 2010

Biology of Reproduction

359

292

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Oocyte and embryo metabolism are closely linked with their subsequent developmental capacity. Lipids are a potent source of cellular energy, yet little is known about lipid metabolism during oocyte maturation and early embryo development. Generation of ATP from lipids occurs within mitochondria via beta-oxidation of fatty acids, with the rate-limiting step catalyzed by carnitine palmitoyl transferase I (CPT1B), a process also requiring carnitine. We sought to investigate the regulation and role of beta-oxidation during oocyte maturation and preimplantation development. Expression of Cpt1b mRNA, assessed by real-time RT-PCR in murine cumulus-oocyte complexes (COCs), increased following hormonal induction of oocyte maturation and ovulation in vivo with human chorionic gonadotropin (5 IU) and in embryos reaching the blastocyst stage. Beta-oxidation, measured by the production of (3)H(2)O from [(3)H]palmitic acid, was significantly increased over that in immature COCs following induction of maturation in vitro with epidermal growth factor (3 ng/ml) and follicle-stimulating hormone (50 mIU/ml). The importance of lipid metabolism for oocyte developmental competence and early embryo development was demonstrated by assessing the rate of embryo development following inhibition or upregulation of beta-oxidation with etomoxir (an inhibitor of CPT1B) or L-carnitine, respectively. Inhibition of beta-oxidation during oocyte maturation or zygote cleavage impaired subsequent blastocyst development. In contrast, L-carnitine supplementation during oocyte maturation significantly increased beta-oxidation, improved developmental competence, and in the absence of a carbohydrate energy supply, significantly increased 2-cell cleavage. Thus, carnitine is an important cofactor for developing oocytes, and fatty acids are an important energy source for oocyte and embryo development.

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Section: Discussionmentioning

confidence: 99%

Beta-Oxidation Is Essential for Mouse Oocyte Developmental Competence and Early Embryo Development1

Dunning

Cashman

Russell

et al. 2010

Biology of Reproduction

359

292

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“…The groups tested were [1] no hormone (no H); [2] 100 ng/mL (porcine) pFSH (100); [3] 100 ng/mL pFSH plus 10% serum (newborn calf serum, Gibco BRL) (100s); [4] 1,000 ng/mL pFSH (1,000); or [5] the positive control containing 1,000 ng/mL pFSH, LH (5 g/mL porcine LH, Lutropin-V, Bioniche Animal Health, Belleville, Ontario, Canada), E 2 -17␤ (1 g/mL E 2 -17␤, Sigma-Aldrich, Oakville, ON, Canada), and 10% serum (FSH lit Estradiol Serum [FLES]). Aliquots of COCs (n ϭ 50) were collected after 6 or 24 hours of IVM.…”

Section: Effect Of Maturation Media Composition On Marker Gene Transcmentioning

confidence: 99%

“…Progress in the treatment of human infertility is directed toward the development of effective culture conditions for the maturation of human oocytes in vitro (1)(2)(3)(4). Despite impressive success in the application of in vitro oocyte maturation methods, there are still concerns regarding their application due to increasing reports highlighting the shortand long-term consequences to development after exposure of mammalian gametes and embryos to culture environments.…”

mentioning

confidence: 99%

Effect of serum and cumulus cell expansion on marker gene transcripts in bovine cumulus-oocyte complexes during maturation in vitro

Calder

Caveney

Sirard

et al. 2005

Fertility and Sterility

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“…Maturation of COCs in vitro (IVM) presents a number of methodological challenges [4][5][6] attributable to the loss of normal cellular interactions and the properties endowed by follicular somatic cells on oocytes. The recent development of a novel system for culturing ovarian follicles in a threedimensional (3D) alginate matrix [7,8] shows great promise in mimicking folliculogenesis in vitro and provides a means to study and manipulate follicle growth and oocyte development directly.…”

Section: Introductionmentioning

confidence: 99%

Increased Beta-Oxidation and Improved Oocyte Developmental Competence in Response to L-Carnitine During Ovarian In Vitro Follicle Development in Mice

Dunning

Akison

Russell

et al. 2011

Biology of Reproduction

102

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Oocyte developmental competence is acquired throughout folliculogenesis and is associated with appropriate differentiation and responsiveness to the luteinizing hormone (LH) surge. The recent development of a novel system for culturing ovarian follicles in a three-dimensional alginate matrix shows promise in phenocopying in vivo folliculogenesis. However, oocytes from follicles grown in vitro have a reduced capacity to complete nuclear maturation and be fertilized compared to oocytes matured in vivo. Oocyte metabolism is closely linked with oocyte quality, and we have recently shown that beta-oxidation of lipids is essential for oocyte developmental competence. Thus we investigated whether upregulation of beta-oxidation by treatment with the fatty acid transport cofactor l-carnitine could improve folliculogenesis and developmental competence of mouse follicles following three-dimensional culture. Ovarian hormones (androstenedione, estradiol, and progesterone) and the induction of cumulus matrix proteins (hyaluronan and ADAMTS1) were similar to in vivo follicles, indicating that appropriate differentiation of follicular cells occurs in cultured follicles after an LH/human chorionic gonadotropin (hCG) stimulus. l-carnitine did not alter survival, growth, or differentiation of follicles. However, l-carnitine supplementation significantly increased beta-oxidation, and markedly improved both fertilization rate and blastocyst development. Together, these results show that appropriate responsiveness of the follicle to the LH/hCG surge occurs following three-dimensional follicle culture but limitations on key metabolic requirements remain. l-carnitine supplementation during in vitro follicle culture increased lipid metabolism and improved oocyte developmental competence.

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In Vitro Maturation and In Vitro Fertilization of Human Oocytes

Cited by 4 publications

References 28 publications

Beta-Oxidation Is Essential for Mouse Oocyte Developmental Competence and Early Embryo Development1

Beta-Oxidation Is Essential for Mouse Oocyte Developmental Competence and Early Embryo Development1

Effect of serum and cumulus cell expansion on marker gene transcripts in bovine cumulus-oocyte complexes during maturation in vitro

Increased Beta-Oxidation and Improved Oocyte Developmental Competence in Response to L-Carnitine During Ovarian In Vitro Follicle Development in Mice

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