In vitro maturation and fertilization of equine oocytes recovered during the breeding season

Dell’Aquila, Maria Elena; Fusco, Sabato; Lacalandra, Giovanni Michele; Maritato, F

doi:10.1016/0093-691x(95)00402-t

Cited by 69 publications

(68 citation statements)

References 30 publications

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“…This result is interesting, considering that conventional IVF has only limited success in equine species (Palmer et al 1991, Dell'aquila et al 1996, Hinrichs et al 2002, McPartlin et al 2009, Mugnier et al 2009). We chose the technique developed by McPartlin et al (2009) in which spermatozoa were hyperactivated using procaine.…”

Section: Discussionmentioning

confidence: 86%

“…The exposure of oocytes to porcine oviductal epithelial cells or oviductal fluid before IVF improves the rate of monospermy (Romar et al 2001. In equine species, IVF rates are low and no repeatable IVF technique is available yet (Palmer et al 1991, Dell'aquila et al 1996, Alm et al 2001, Hinrichs et al 2002, McPartlin et al 2009). We have shown a beneficial effect of homologous and heterologous oviduct cells on equine IVF rates (Mugnier et al 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Ambruosi¹,

Accogli²,

Douet³

et al. 2013

Reproduction

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Oviductal environment affects preparation of gametes for fertilization, fertilization itself, and subsequent embryonic development. The aim of this study was to evaluate the effect of oviductal fluid and the possible involvement of deleted in malignant brain tumor 1 (DMBT1) on IVF in porcine and equine species that represent divergent IVF models. We first performed IVF after pre-incubation of oocytes with or without oviductal fluid supplemented or not with antibodies directed against DMBT1. We showed that oviductal fluid induces an increase in the monospermic fertilization rate and that this effect is canceled by the addition of antibodies, in both porcine and equine species. Moreover, pre-incubation of oocytes with recombinant DMBT1 induces an increase in the monospermic fertilization rate in the pig, confirming an involvement of DMBT1 in the fertilization process. The presence of DMBT1 in the oviduct at different stages of the estrus cycle was shown by western blot and confirmed by immunohistochemical analysis of ampulla and isthmus regions. The presence of DMBT1 in cumulus-oocyte complexes was shown by western blot analysis, and the localization of DMBT1 in the zona pellucida and cytoplasm of equine and porcine oocytes was observed using immunofluorescence analysis and confocal microscopy. Moreover, we showed an interaction between DMBT1 and porcine spermatozoa using surface plasmon resonance studies. Finally, a bioinformatic and phylogenetic analysis allowed us to identify the DMBT1 protein as well as a DMBT1-like protein in several mammals. Our results strongly suggest an important role of DMBT1 in the process of fertilization.

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Section: Discussionmentioning

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Ambruosi¹,

Accogli²,

Douet³

et al. 2013

Reproduction

View full text Add to dashboard Cite

show abstract

“…The third step is IVF of oocytes. Several attempts to establish an efficient conventional IVF technique in the equine have been performed, but IVF rates remained quite low and IVF techniques were not repeatable [24][25][26][27][28][29][30]. Intracytoplasmic Sperm Injection (ICSI) has been widely adopted to generate horse embryos in vitro, both for scientific purposes and in the horse breeding industry [11,31].…”

Section: Introductionmentioning

confidence: 99%

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

et al. 2016

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“…Attempts to induce capacitation (and subsequent acrosome reaction) of stallion spermatozoa in vitro have been reported by treatments with the following substances; calcium ionophore A23187 (IA) [3][4][5][6][7][8][9], liposomes [10][11][12], and heparin [7,8,[13][14][15][16], and by incubation with oviductal epithelial cells [6]. Among them, the pretreatment of spermatozoa with IA before IVF appears to be most promising for successful IVF in equine species, since two foals have been born from IVF embryos which were derived from oocytes that had been matured in vivo and inseminated with spermatozoa treated with IA [5,17].…”

mentioning

confidence: 99%

Assay for Penetrability of Frozen-thawed Stallion Spermatozoa into Zona-free Hamster Oocytes.

Matsukawa

Takahashi

Hanada

2002

Journal of Reproduction and Development

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Abstract. In Japan, low availability of equine oocytes makes it very difficult to improve the in vitro fertilization (IVF) system of horses. In this situation, an assay for sperm penetrability into zona-free hamster oocytes could be useful as a simulator of the IVF system. The aim of this study was to clarify the optimum conditions of the pretreatment of frozen-thawed stallion spermatozoa with caffeine and ionophore A23187 (IA) in the penetration assay. Frozen-thawed stallion spermatozoa were washed and then pretreated with IA in modified BO solution (mBO) supplemented with caffeine. After the pretreatment, the spermatozoa were used for the penetration assay with zona-free hamster oocytes. At first, effect of increasing concentrations of caffeine (0-10 mM) were examined on the penetration of spermatozoa pretreated with 1 µM IA for 60 sec. The highest penetration rate after 10 h co-culture with oocytes was obtained for spermatozoa penetrated in the presence of 1 mM caffeine (49.0%). Next, when spermatozoa were pretreated with 0-1.0 µM IA for 0-180 sec in the presence of 1 mM caffeine, significantly higher penetration rates after 10 h co-culture with oocytes were obtained under the condition of 0.1 µM IA for 120 sec (52.2%) and 1.0 µM IA for 60 sec (40.9%). When oocytes were fixed 2, 4, 6, 8 and 10 h after IVF using spermatozoa treated with 0.1 µM IA for 120 sec in mBO containing 1 mM caffeine, the penetration rates increased significantly from 2 h (10.4%) to 4 h (21.7%) and from 6 h (27.5%) to 8 h (49.0%). These results indicate that pretreatment with 0.1 µM IA for 120 sec in the presence of 1 mM caffeine effectively promotes the penetration of frozen-thawed stallion spermatozoa into zona-free hamster oocytes, and that under this condition, spermatozoa might complete the acrosome reaction during 6 to 8 h of incubation after IA treatment.

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In vitro maturation and fertilization of equine oocytes recovered during the breeding season

Cited by 69 publications

References 30 publications

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Deleted in malignant brain tumor 1 is secreted in the oviduct and involved in the mechanism of fertilization in equine and porcine species

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

Assay for Penetrability of Frozen-thawed Stallion Spermatozoa into Zona-free Hamster Oocytes.

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