In vitro mass propagation of Cannabis sativa L.: A protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants

Lata, Hemant; Chandra, Suman; Techen, Natascha; Khan, Ikhlas A.; ElSohly, Mahmoud A.

doi:10.1016/j.jarmap.2015.12.001

Cited by 81 publications

(99 citation statements)

References 42 publications

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“…The present investigation suggests that the IAA showed the least response in root formation. In comparison with other auxins, a similar observation was reported 28 . Tissue culture techniques can play an important role in clonal propagation of elite genotypes of has diverse medicinal applications and eventually due to overexploitation and irregular concern this plant is facing local extinction.…”

Section: Methodssupporting

confidence: 82%

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2019

IJPSR

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The present study was developed a suitable protocol for callus induction and plant regeneration of Cleome gynandra L. from leaf explants. Callus was initiated from leaf explants on Murashige and Skoog (MS) medium supplemented with different concentration and combinations of plant growth regulators. They are using (5-25 µM) of BAP (6-benzyladenine), KN (kinetin), and TDZ (thidiazuron) cytokinins along with (2-10 µM) different auxins [NAA (α-naphthalene acetic acid), IAA (indole-3 acetic acid), IBA (indole-3-butyric acid)]. Maximum regenerative callus (95%) was observed on TDZ + NAA (10 µM + 2 µM). Organogenic calli were transferred to shoot induction medium containing a different concentration of cytokinins (BAP, KIN, TDZ) ranging from 5-25 µM. Among the cytokinins tested BAP 10 µM produced number of shoots (35.2) with maximum shoot induction frequency (98%). Isolated shoots were carefully transferred to half-strength MS medium supplemented with auxins (IAA, IBA, and NAA) ranging from 2-10 µM. The maximum numbers of roots (8.8) were observed on 4 µM IBA. The rooted plantlets were successfully acclimatization.

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Section: Methodssupporting

confidence: 82%

Untitled

2019

IJPSR

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“…Given the obligate outcrossing nature of Cannabis [34] as well as heterozygosity within the gene pool [3, 35], seed-grown Cannabis botanical raw material can be highly variable in content and composition of target and non-target active ingredients [31, 36]. Thus, clonal propagation using callus- [37] or nodal- (axillary buds) [38] mediated regeneration is favoured over seed propagation [22], with explants typically grown under environmentally controlled conditions in an effort to minimise genetic by environment interactions and to maximise product uniformity [31]. …”

Section: Cannabis Drug Formsmentioning

confidence: 99%

Developing Robust Standardised Analytical Procedures for Cannabinoid Quantification: Laying the Foundations for an Emerging Cannabis-Based Pharmaceutical Industry

Welling¹,

Liu²,

Hazekamp³

et al. 2019

Med Cannabis Cannabinoids

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The plant genus Cannabis is a prolific producer of unique pharmaceutically relevant metabolites, commonly referred to as cannabinoids. Robust and standardised methods for the quantification of cannabinoids within botanical and drug forms is a critical step forward for an emerging Cannabis-based pharmaceutical industry, which is poised for rapid expansion. Despite a growing body of analytical methods for the quantification of cannabinoids, few have been validated using internationally accredited guidelines. Moreover, standardised methods have yet to be developed for application at various stages of manufacture as well as for different levels of processing and refinement. Validation parameters for establishing robust standardised methods for cannabinoid quantification within Cannabis-based drug forms are critically discussed. Determining an appropriate level of specificity (discrimination) among heterogeneous botanical matrices as well as evaluating accuracy (recovery) and inter-laboratory precision (reproducibility) within strict and volatile regulatory environments are potential obstacles to the establishment of robust analytical procedures. We argue that while some of these challenges remain unique to Cannabis, others are common to botanical-based drug development and manufacture. In order to address potential barriers to analytical method standardisation, a collaborative research initiative inclusive of academic and commercial stakeholders is proposed.

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“…However, the efficiency of completely regenerated plants was very low [14]. With the use of the cytokinin meta-topolin, the group of ElSohly [17] was successful. They were able to establish a one-step protocol for plant regeneration out of axillary bud explants with direct organogenesis.…”

Section: Transgenic Cannabis Plantsmentioning

confidence: 99%

Current Perspectives on Biotechnological Cannabinoid Production in Plants

et al. 2018

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The plant contains a number of psychoactive chemical compounds, the cannabinoids, which possess a significant pharmaceutical potential. Recently, the usage of for medicinal purposes was legalized in many countries. Thus, the study on the influence of different cannabinoids in combination with other -derived compounds with respect to the treatment of various diseases becomes increasingly important. Besides the production of distinct cannabinoids in a heterologous host, like tobacco or yeast, transgenic plants would be a suitable alternative to modify and therefore optimize the cannabinoid profile. This perspective highlights the current efforts on cell culture systems, propagation, and transformation of the plant and reveals the resulting opportunities concerning biotechnological production of cannabinoids. Furthermore, alternative platform organisms for the heterologous production of cannabinoids, like tobacco, are considered and evaluated.

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In vitro mass propagation of Cannabis sativa L.: A protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants

Cited by 81 publications

References 42 publications

Untitled

Untitled

Developing Robust Standardised Analytical Procedures for Cannabinoid Quantification: Laying the Foundations for an Emerging Cannabis-Based Pharmaceutical Industry

Current Perspectives on Biotechnological Cannabinoid Production in Plants

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